FastQCFastQC Report
Wed 25 May 2016
SRR1779758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18247
Sequences flagged as poor quality0
Sequence length76
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG453.6379788E-1269.98077442
TCGTCTG207.7316916E-569.98077445
CTCGGTG401.2369128E-1069.98077444
CTTGTAG207.7316916E-569.98077435
ATACCCA207.7316916E-569.9807744
TTACGCT207.7316916E-569.98077413
TGGTCGT150.002192180469.9807749
TGGTTGC150.002192180469.9807749
TTGTAGA150.002192180469.9807736
GTATATT150.002192180469.9807738
AGAACGG150.002192180469.9807727
CTGGTGG150.002192180469.9807745
CTGTGTA150.002192180469.9807734
CTTACGC252.7346614E-669.9807712
TTTGGTG150.002192180469.9807744
GGTGGTT150.002192180469.9807747
AAGTGTA150.002192180469.9807734
GTGGTTG150.002192180469.9807748
TGGTGTA150.002192180469.9807746
TACTCAT150.002192180469.980775
Produced by FastQC (version 0.11.5)