##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779758_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18247
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.977475749438263	23.0	12.0	33.0	12.0	34.0
2	23.347728393708554	23.0	12.0	33.0	12.0	34.0
3	23.541732887597963	23.0	11.0	32.0	11.0	34.0
4	23.435304433605523	23.0	12.0	33.0	11.0	34.0
5	23.693045432125828	23.0	11.0	34.0	11.0	34.0
6	24.63435085219488	26.0	12.0	36.0	11.0	38.0
7	25.780402257905408	27.0	12.0	36.0	12.0	38.0
8	24.74236860853839	26.0	11.0	36.0	11.0	38.0
9	26.380117279552803	28.0	12.0	37.0	12.0	38.0
10-11	25.424343727736066	27.0	11.0	37.0	11.0	38.0
12-13	25.904778867759084	28.0	11.0	36.0	11.0	38.0
14-15	24.620019729270567	26.0	11.0	36.0	11.0	38.0
16-17	24.87724009426207	26.5	11.0	35.5	11.0	38.0
18-19	25.262865128514278	27.0	11.5	36.5	11.0	38.0
20-21	24.341645201951003	25.5	11.0	35.0	11.0	38.0
22-23	24.613388502219543	25.5	11.0	35.5	11.0	38.0
24-25	24.553214226996218	25.5	11.0	36.0	11.0	38.0
26-27	23.662656875102755	23.0	11.0	34.5	10.0	38.0
28-29	24.50115087411629	26.0	11.0	36.5	11.0	38.0
30-31	25.026661917027454	26.0	11.0	37.0	11.0	38.0
32-33	25.221022633857622	26.0	11.0	37.0	11.0	38.0
34-35	25.179947388611826	26.5	11.0	37.0	11.0	38.0
36-37	24.446785773003782	25.5	11.0	35.0	11.0	38.0
38-39	24.390502548364115	25.5	11.0	34.0	11.0	37.5
40-41	23.73214775031512	25.0	11.0	34.0	11.0	37.5
42-43	24.223982024442375	26.0	11.0	35.0	11.0	38.0
44-45	25.096646024003945	26.0	11.0	37.0	11.0	38.0
46-47	24.61555324162876	26.0	11.0	35.5	11.0	38.0
48-49	23.717433002685375	24.0	11.0	35.0	11.0	38.0
50-51	23.102098975174002	23.0	11.0	34.0	11.0	37.5
52-53	24.031868252315448	24.0	11.0	35.5	11.0	38.0
54-55	23.85082479311668	24.0	11.0	35.0	11.0	38.0
56-57	24.19855318682523	24.5	11.0	35.5	11.0	38.0
58-59	24.463199430043296	25.0	11.0	37.0	11.0	38.0
60-61	25.051761933468512	26.0	11.0	37.0	11.0	38.0
62-63	25.08080780402258	26.5	11.0	37.0	11.0	38.0
64-65	25.05398147640708	26.0	11.0	37.0	11.0	38.0
66-67	24.30714638022689	25.0	11.0	35.5	11.0	38.0
68-69	24.005288540582015	25.0	11.0	35.5	11.0	38.0
70-71	23.750835753822546	24.5	11.0	34.0	11.0	38.0
72-73	23.274894503206006	24.0	11.0	34.0	11.0	37.5
74-75	23.307721817285035	23.5	11.0	35.0	11.0	38.0
76	23.028991067024716	23.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
11	4.0
12	20.0
13	123.0
14	375.0
15	664.0
16	1054.0
17	1382.0
18	1803.0
19	1779.0
20	1424.0
21	1074.0
22	753.0
23	505.0
24	424.0
25	328.0
26	256.0
27	267.0
28	278.0
29	301.0
30	326.0
31	416.0
32	602.0
33	661.0
34	904.0
35	1063.0
36	1023.0
37	438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.45881514769551	20.90754644599112	13.952978571820024	40.68065983449334
2	13.169288102153779	15.109333041047842	50.11234723516195	21.609031621636436
3	13.492628925302789	12.040335397599605	51.246780292650854	23.22025538444676
4	12.845947279004768	25.0232914999726	23.669644325094534	38.4611168959281
5	12.708938455636542	20.35403079958349	45.75546665205239	21.181564092727573
6	20.41979503480024	35.4359620759577	15.651887981586013	28.492354907656054
7	19.674467035677097	17.92075409656382	16.64931221570669	45.75546665205239
8	16.578067627555214	33.528799254671995	17.553570449936977	32.33956266783581
9	16.238285745602017	14.5722584534444	17.126102921028114	52.06335287992546
10-11	18.34822162547268	21.524086151148133	29.103414259878335	31.02427796350085
12-13	16.723296980325532	14.703786923877898	34.93999013536472	33.632925960431855
14-15	24.705431029758316	20.866443798980654	22.066641091686304	32.36148407957472
16-17	30.248808023236695	15.440894393598947	21.30213185729161	33.00816572587274
18-19	18.46878939003672	14.547596865238122	30.876308434263166	36.107305310461996
20-21	29.993971611771798	14.80243327670302	22.516030032334083	32.6875650791911
22-23	32.19981366800022	13.287115690250454	20.54584315229901	33.967227489450316
24-25	20.29100674083411	22.135145503370417	19.23603880089878	38.337808954896694
26-27	28.763632377925134	21.03633473995725	21.455581739464023	28.74445114265359
28-29	21.94059297418754	20.77875815202499	23.316161560804517	33.96448731298295
30-31	21.042391691557285	19.83668100731647	25.413092921930236	33.70783437919601
32-33	22.239272209130267	18.83597303666356	25.132898558667176	33.791856195539
34-35	21.37063626897572	19.526497506439412	24.861621088398092	34.24124513618677
36-37	29.073272318737324	13.295336219652546	31.125664492793337	26.505726968816788
38-39	28.67868690743684	10.927823751849619	37.75141119088069	22.64207814983285
40-41	24.09163150106867	23.461390913574835	22.428344385378416	30.01863319997808
42-43	16.73973803912972	24.061489559927658	26.837288321367897	32.36148407957472
44-45	17.18364662684277	15.931385981257193	31.97511919767633	34.909848194223706
46-47	28.382747848961472	14.914780511864963	25.228804735024934	31.47366690414863
48-49	27.977201731791528	14.827094864909302	29.706253082698524	27.489450320600646
50-51	41.193072833890504	12.36641639721598	23.255877678522495	23.184633090371022
52-53	17.120622568093385	18.296158272592756	30.267989258508248	34.31522990080561
54-55	27.714144790924532	15.035348276429003	24.546500794651173	32.70400613799529
56-57	27.429166438318624	16.08757603989697	22.822929796678906	33.660327725105496
58-59	17.797446155532416	20.25538444675837	28.785553789664053	33.16161560804516
60-61	16.93977092124733	17.057598509344	28.508795966460237	37.493834602948425
62-63	17.942675508302734	20.707513563873515	27.10856579163698	34.24124513618677
64-65	18.046802214062584	22.647558502767577	26.14402367512468	33.16161560804516
66-67	15.476516687674685	33.0191264317422	18.00021921411739	33.504137666465724
68-69	16.417705906536934	31.508839249006442	18.152665478963957	33.92078936549267
70-71	16.961692332986246	33.73431248972434	16.816462980215928	32.48753219707349
72-73	16.473940921795364	36.65808078040226	15.706691510933304	31.161286786869073
74-75	18.953800624760234	25.116457499862992	20.710253740340878	35.219488135035895
76	20.91850715186058	21.943333150654905	23.46687126650956	33.671288430974954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	1.0
19	2.5
20	4.0
21	4.0
22	4.5
23	9.5
24	17.0
25	20.0
26	23.5
27	32.0
28	43.5
29	50.0
30	62.5
31	90.5
32	139.5
33	173.0
34	184.5
35	220.5
36	267.0
37	289.0
38	355.5
39	457.0
40	495.5
41	550.0
42	601.0
43	639.5
44	693.5
45	726.5
46	744.0
47	761.0
48	782.5
49	765.5
50	744.0
51	743.0
52	709.5
53	655.5
54	634.0
55	628.0
56	597.5
57	536.0
58	499.0
59	531.5
60	548.5
61	526.0
62	519.0
63	526.5
64	521.5
65	505.0
66	496.0
67	491.0
68	495.5
69	494.5
70	458.5
71	428.0
72	410.0
73	375.5
74	320.5
75	282.0
76	245.5
77	186.0
78	144.5
79	126.0
80	105.0
81	69.0
82	41.0
83	28.0
84	22.0
85	11.5
86	5.5
87	4.0
88	3.0
89	1.0
90	1.0
91	1.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.002740176467364498
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.024661588206280484
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	18247.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.6985805885899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.70316622691293	99.40264153011455
2	0.29133685136323656	0.5809174110812736
3	0.005496921723834653	0.01644105880418699
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.005480352934728996	0.0
15	0.0	0.0	0.0	0.005480352934728996	0.0
16	0.0	0.0	0.0	0.005480352934728996	0.0
17	0.0	0.0	0.0	0.01644105880418699	0.0
18	0.0	0.0	0.0	0.01644105880418699	0.0
19	0.0	0.0	0.0	0.027401764673644982	0.0
20	0.0	0.0	0.0	0.027401764673644982	0.0
21	0.0	0.0	0.0	0.027401764673644982	0.0
22	0.0	0.0	0.0	0.06028388228201896	0.0
23	0.0	0.0	0.0	0.06576423521674796	0.0
24	0.0	0.0	0.0	0.07672494108620595	0.0
25	0.0	0.0	0.0	0.07672494108620595	0.0
26	0.0	0.0	0.0	0.08768564695566394	0.0
27	0.0	0.0	0.0	0.12056776456403792	0.0
28	0.0	0.0	0.0	0.1644105880418699	0.0
29	0.0	0.0	0.0	0.1808516468460569	0.0
30	0.0	0.0	0.0	0.20825341151970186	0.0
31	0.0	0.0	0.0	0.23565517619334686	0.0
32	0.0	0.0	0.0	0.29593905847536583	0.0
33	0.0	0.0	0.0	0.3397818819531978	0.0
34	0.0	0.0	0.0	0.40554611716994576	0.0
35	0.0	0.0	0.0	0.5041924699950677	0.0
36	0.0	0.0	0.0	0.5809174110812736	0.0
37	0.0	0.0	0.0	0.8439743519482654	0.0
38	0.0	0.0	0.0	1.0631884693374254	0.0
39	0.0	0.0	0.0	1.2385597632487533	0.0
40	0.0	0.0	0.0	1.441332821833726	0.0
41	0.0	0.0	0.0	1.589302351071409	0.0
42	0.0	0.0	0.0	1.7865950567216529	0.0
43	0.0	0.0	0.0	2.0003288211760837	0.0
44	0.0	0.0	0.0	2.1647394092179537	0.0
45	0.0	0.0	0.0	2.3236696443250944	0.0
46	0.0	0.0	0.0	2.4716391735627776	0.0
47	0.0	0.0	0.0	2.7018139968213952	0.0
48	0.0	0.0	0.0	2.844303173124349	0.0
49	0.0	0.0	0.0	2.9648709376883873	0.0
50	0.0	0.0	0.0	3.123801172795528	0.0
51	0.0	0.0	0.0	3.321093878445772	0.0
52	0.0	0.0	0.0	3.458102701813997	0.0
53	0.0	0.0	0.0	3.699238230942073	0.0
54	0.0	0.0	0.0	3.8526881131144846	0.0
55	0.0	0.0	0.0	3.9513344659396066	0.0
56	0.0	0.0	0.0	4.071902230503644	0.0
57	0.0	0.0	0.0	4.236312818545514	0.0
58	0.0	0.0	0.0	4.384282347783198	0.0
59	0.0	0.0	0.0	4.515810818216693	0.0
60	0.0	0.0	0.0	4.554173288759796	0.0
61	0.0	0.0	0.0	4.647339288650189	0.0
62	0.0	0.0	0.0	4.674741053323834	0.0
63	0.0	0.0	0.0	4.75146599441004	0.0
64	0.0	0.0	0.0	4.80626952375733	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGG	45	3.6379788E-12	69.980774	42
TCGTCTG	20	7.7316916E-5	69.980774	45
CTCGGTG	40	1.2369128E-10	69.980774	44
CTTGTAG	20	7.7316916E-5	69.980774	35
ATACCCA	20	7.7316916E-5	69.980774	4
TTACGCT	20	7.7316916E-5	69.980774	13
TGGTCGT	15	0.0021921804	69.98077	49
TGGTTGC	15	0.0021921804	69.98077	49
TTGTAGA	15	0.0021921804	69.98077	36
GTATATT	15	0.0021921804	69.98077	38
AGAACGG	15	0.0021921804	69.98077	27
CTGGTGG	15	0.0021921804	69.98077	45
CTGTGTA	15	0.0021921804	69.98077	34
CTTACGC	25	2.7346614E-6	69.98077	12
TTTGGTG	15	0.0021921804	69.98077	44
GGTGGTT	15	0.0021921804	69.98077	47
AAGTGTA	15	0.0021921804	69.98077	34
GTGGTTG	15	0.0021921804	69.98077	48
TGGTGTA	15	0.0021921804	69.98077	46
TACTCAT	15	0.0021921804	69.98077	5
>>END_MODULE