FastQCFastQC Report
Wed 25 May 2016
SRR1779758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18247
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT572731.385981257192963No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG7824.285635994958075No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT7203.9458541130048777Illumina PCR Primer Index 10 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC4162.2798268208472625Illumina PCR Primer Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC4072.2305036444347013No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3091.69342905683126Illumina PCR Primer Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2831.550939880528306No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC1540.8439743519482654Illumina PCR Primer Index 10 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1420.7782101167315175No Hit
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT1410.7727297637967885Illumina PCR Primer Index 10 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1300.7124458815147695No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT1180.6466816462980216No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1110.6083191757549187No Hit
TCTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT810.4439085877130487No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT550.3014194114100948No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG380.20825341151970186No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATGATCTCGTATGCCGT370.20277305858497288No Hit
CTCTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG360.19729270565024387No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAGCTCGTATGCCGT320.17537129391132789No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGCTTATCTCGTATGCCGT320.17537129391132789TruSeq Adapter, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCGT310.1698909409765989No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGC270.1479695292376829No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCG260.1424891763029539No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGAGTATCTCGTATGCCGT260.1424891763029539No Hit
CTTATACACATCTCCGAGCCCACGAGACATTAGATTATCTCGTATGCCGT250.1370088233682249No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGACTATCTCGTATGCCGT250.1370088233682249No Hit
CTTATTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG240.13152847043349591No Hit
CTTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC220.12056776456403794No Hit
CTTATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC210.11508741162930891No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGTCTT210.11508741162930891No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATCATCTCGTATGCCGT200.10960705869457993No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAGATTATCTCGTATGCCGT190.10412670575985093No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG190.10412670575985093No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAT150.002189792770.0000127
TATATTA150.002189792770.0000131
CAGTATA150.002189792770.0000128
TGATCTC150.002189792770.0000135
GTATATT150.002189792770.0000130
AGTATAT150.002189792770.0000129
ATCGGGC150.002189792770.0000161
ACAGAAG207.721178E-570.027
GACAGAA207.721178E-570.026
CTTAATA207.721178E-570.02
TAATACA207.721178E-570.04
AATACAC207.721178E-570.05
TTAATAC207.721178E-570.03
TCTTTAT550.070.01
TCTTAAT207.721178E-570.01
CTATACA750.065.3333361
CTTTACA308.054863E-658.3333361
TTACACA308.054863E-658.3333363
TTTACAC308.054863E-658.3333362
AGACAGA252.3288498E-455.99999625
Produced by FastQC (version 0.11.5)