FastQCFastQC Report
Wed 25 May 2016
SRR1779757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63964
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAT150.002215282870.0000135
CGACTAG150.002215282870.0000122
CCGTATC308.290328E-658.33333655
CGTATCA252.3739437E-455.99999656
CGCCGTA252.3739437E-455.99999653
TCGGTGG509.567884E-1055.99999645
GCCGTAT252.3739437E-455.99999654
CGGTGGT452.6499038E-854.44444346
AGTGTAG452.6499038E-854.44444335
CTTGTCA200.00692220552.51
GTACATC200.00692220552.538
CCGATGC200.00692220552.515
CGGCATC200.00692220552.551
TCTTAAC200.00692220552.567
GTATATT200.00692220552.510
GCTCCGG200.00692220552.511
GCCGACT305.837623E-446.66666820
CAGTGTA305.837623E-446.66666834
TCGCCGT451.6607664E-646.66666452
ATCTCGG451.6607664E-646.66666442
Produced by FastQC (version 0.11.5)