##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779757_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	63964
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.38101119379651	34.0	32.0	34.0	21.0	34.0
2	28.334860233881557	34.0	23.0	34.0	12.0	34.0
3	27.47933212431993	33.0	23.0	34.0	11.0	34.0
4	30.095835157275967	34.0	31.0	34.0	21.0	34.0
5	29.415483709586642	34.0	27.0	34.0	11.0	34.0
6	32.136905134137955	37.0	31.0	38.0	12.0	38.0
7	33.12553936589332	37.0	34.0	38.0	21.0	38.0
8	32.41207554249265	37.0	31.0	38.0	12.0	38.0
9	33.87188105809518	38.0	34.0	38.0	21.0	38.0
10-11	33.581225376774434	38.0	34.0	38.0	21.0	38.0
12-13	32.452504533800266	37.5	30.5	38.0	16.0	38.0
14-15	32.69272559564755	37.0	32.5	38.0	16.0	38.0
16-17	32.32189200175098	37.5	30.0	38.0	16.0	38.0
18-19	32.80383496967044	37.0	32.5	38.0	16.0	38.0
20-21	31.846702832843476	36.5	30.0	38.0	16.0	38.0
22-23	31.82479207053968	36.5	28.5	38.0	16.0	38.0
24-25	33.28343286848852	37.0	33.0	38.0	21.0	38.0
26-27	31.537145894565693	37.0	27.5	38.0	11.0	38.0
28-29	33.90553279969983	37.5	34.5	38.0	21.5	38.0
30-31	34.40654117941342	38.0	36.0	38.0	23.0	38.0
32-33	34.49528641110625	38.0	36.0	38.0	23.0	38.0
34-35	34.53953004815209	38.0	36.0	38.0	23.5	38.0
36-37	32.26334344318679	37.0	30.0	38.0	16.0	38.0
38-39	30.2176380464011	35.0	24.0	38.0	11.0	38.0
40-41	31.78652835970233	36.5	29.0	38.0	11.0	38.0
42-43	32.959649177662435	37.0	30.5	38.0	17.0	38.0
44-45	34.59693733975361	38.0	36.0	38.0	25.0	38.0
46-47	32.97806578700519	37.5	31.5	38.0	18.0	38.0
48-49	32.33605621912326	37.0	30.0	38.0	17.0	38.0
50-51	30.09619473453818	35.5	23.5	38.0	11.0	38.0
52-53	33.78660652867238	37.0	34.5	38.0	22.0	38.0
54-55	32.75627696829467	37.0	30.5	38.0	17.0	38.0
56-57	33.15859702332562	37.5	31.5	38.0	17.0	38.0
58-59	34.39455787630542	38.0	35.0	38.0	23.5	38.0
60-61	34.8010287036458	38.0	37.0	38.0	24.5	38.0
62-63	34.73022325057845	38.0	36.5	38.0	24.5	38.0
64-65	34.39129823025452	38.0	36.0	38.0	23.0	38.0
66-67	32.354699518479144	37.5	31.0	38.0	11.0	38.0
68-69	32.370090988681135	37.0	31.0	38.0	11.0	38.0
70-71	31.732396347945716	37.0	28.5	38.0	11.0	38.0
72-73	30.565966793821524	36.5	25.0	38.0	11.0	38.0
74-75	32.82799699831155	37.0	30.5	38.0	17.0	38.0
76	33.61334500656619	37.0	34.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	89.0
14	209.0
15	331.0
16	595.0
17	826.0
18	1082.0
19	1110.0
20	987.0
21	745.0
22	704.0
23	665.0
24	605.0
25	625.0
26	696.0
27	922.0
28	1112.0
29	1358.0
30	1847.0
31	2520.0
32	3534.0
33	5282.0
34	7722.0
35	10998.0
36	12779.0
37	6611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.115189794259265	19.787693077356014	12.13964104808955	29.957476080295166
2	20.6631855418673	17.300356450503408	38.543555750109434	23.492902257519855
3	20.25201675942718	18.544806453630166	35.25107873178663	25.952098055156025
4	13.476330435870176	18.916890751047465	34.42717778750547	33.179601025576886
5	13.65455568757426	30.132574573197424	39.74892126821337	16.463948471014948
6	26.25226690013132	33.66424863985992	17.853792758426614	22.22969170158214
7	26.070914889625413	28.38627978237759	20.658495403664563	24.884309924332438
8	22.243762116190357	31.739728597336004	19.10605965855794	26.9104496279157
9	22.82221249452817	16.381089362766556	20.089425301732224	40.70727284097305
10-11	23.421768494778313	24.50519041961103	27.570195735100995	24.50284535050966
12-13	23.43349384028516	21.118128947532988	29.279751109999374	26.16862610218248
14-15	22.382902882871615	23.51244450003127	24.436401725970857	29.668250891126256
16-17	23.420986805077856	24.59117628666125	26.73925958351573	25.24857732474517
18-19	21.044650115690075	24.63964104808955	28.51291351385154	25.802795322368834
20-21	22.901944843974736	24.681852291914204	27.939934963416924	24.47626790069414
22-23	23.21071227565506	23.700831717841286	28.37689950597211	24.711556500531547
24-25	21.28384716402977	26.448471014945905	25.43383778375336	26.833844037270964
26-27	21.975642548933774	24.93277468576074	28.407385404289915	24.684197361015574
28-29	21.619973735226065	26.121724720155086	27.456069038834347	24.802232505784506
30-31	21.473797761240697	26.326527421674694	27.09414670752298	25.105528109561632
32-33	21.630135701331998	26.17487961978613	27.000343943468202	25.19464073541367
34-35	21.734882121193174	25.874710774810833	27.185604402476393	25.204802701519608
36-37	22.506409855543744	24.015070977424802	30.55390532174348	22.924613845287976
38-39	22.56738165217935	23.055937714964667	32.908354699518476	21.468325933337503
40-41	21.977987618035144	24.180007504221123	26.491463948471015	27.350540929272714
42-43	20.395847664311177	25.046119692326936	27.36070289537865	27.19732974798324
44-45	20.736664373710212	24.960133825276717	28.660652867237822	25.64254893377525
46-47	22.123381902320055	23.94393721468326	29.443124257394786	24.489556625601903
48-49	22.300043774623227	24.25504971546495	29.51503970983678	23.929866800075043
50-51	23.315458695516227	22.6330435870177	31.61231317616159	22.43918454130448
52-53	20.25670689762992	25.00469013820274	29.042117441060594	25.696485523106748
54-55	21.437840035019697	24.44969045087862	28.92642736539303	25.186042148708648
56-57	21.326840097554875	24.761584641360766	28.518385341754737	25.39318991932962
58-59	19.708742417609905	25.78794321806016	28.68410355825152	25.81921080607842
60-61	19.545369270214497	25.5464011006191	28.41911074979676	26.489118879369645
62-63	19.308517290976173	25.73557000812957	28.587955725095366	26.36795697579889
64-65	20.01203802138703	26.137358514164216	27.474047901944843	26.376555562503906
66-67	19.117003314364332	25.09849290225752	25.12116190357076	30.663341879807394
68-69	19.091136509326287	25.553088697446803	25.48116762300849	29.87460717021842
70-71	19.471890438371585	25.15008442248765	24.80145081608405	30.57657432305672
72-73	19.44062285035332	24.25739478456632	23.983803389406543	32.31817897567382
74-75	20.79763617034582	25.882527671815396	25.656619348383465	27.66321680945532
76	21.899818647989495	25.196985804515037	26.103745857044586	26.799449690450878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	2.0
16	10.0
17	16.0
18	13.5
19	20.0
20	39.0
21	49.0
22	63.0
23	86.5
24	120.0
25	144.0
26	187.0
27	281.5
28	394.0
29	455.0
30	550.0
31	746.5
32	957.5
33	1067.0
34	1222.5
35	1526.0
36	1819.5
37	1965.0
38	2188.0
39	2624.5
40	3048.0
41	3387.5
42	3517.0
43	3574.5
44	3722.5
45	3809.5
46	3806.0
47	3781.0
48	3669.5
49	3417.0
50	3251.0
51	3110.0
52	2743.0
53	2281.5
54	2046.0
55	1933.5
56	1693.5
57	1430.5
58	1295.0
59	1203.5
60	1023.0
61	890.5
62	847.0
63	787.5
64	690.5
65	619.5
66	596.0
67	606.0
68	569.0
69	521.0
70	481.0
71	452.0
72	437.5
73	389.0
74	321.0
75	287.0
76	270.5
77	207.0
78	149.5
79	139.0
80	117.0
81	87.0
82	59.0
83	39.0
84	32.5
85	26.0
86	16.5
87	7.0
88	5.5
89	3.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.007816897004565067
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	63964.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.34850853605153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.89137277574356	95.29579138265274
2	1.7280143894135027	3.364392470764805
3	0.27140746450825465	0.7926333562628979
4	0.051390762510438746	0.20011256331686575
5	0.024089419926768163	0.11725345506847602
6	0.014453651956060899	0.08442248764930273
7	0.011241729299158476	0.07660559064473767
8	0.004817883985353632	0.037521105621912326
9	0.0016059613284512108	0.014070414608217122
>10	0.0016059613284512108	0.01719717341004315
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.004690138202739041	0.0
12	0.0	0.0	0.0	0.004690138202739041	0.0
13	0.0	0.0	0.0	0.004690138202739041	0.0
14	0.0	0.0	0.0	0.004690138202739041	0.0
15	0.0	0.0	0.0	0.004690138202739041	0.0
16	0.0	0.0	0.0	0.004690138202739041	0.0
17	0.0	0.0	0.0	0.004690138202739041	0.0
18	0.0	0.0	0.0	0.004690138202739041	0.0
19	0.0	0.0	0.0	0.010943655806391095	0.0
20	0.0	0.0	0.0	0.010943655806391095	0.0
21	0.0	0.0	0.0	0.010943655806391095	0.0
22	0.0	0.0	0.0	0.02657744981552123	0.0
23	0.0	0.0	0.0	0.028140829216434245	0.0
24	0.0	0.0	0.0	0.02970420861734726	0.0
25	0.0	0.0	0.0	0.02970420861734726	0.0
26	0.0	0.0	0.0	0.03283096741917328	0.0
27	0.0	0.0	0.0	0.040647864423738356	0.0
28	0.0	0.0	0.0	0.04377462322556438	0.0
29	0.0	0.0	0.0	0.054718279031955475	0.0
30	0.0	0.0	0.0	0.06566193483834656	0.0
31	0.0	0.0	0.0	0.07660559064473767	0.0
32	0.0	0.0	0.0	0.08598586705021574	0.0
33	0.0	0.0	0.0	0.09536614345569383	0.0
34	0.0	0.0	0.0	0.10787317866299793	0.0
35	0.0	0.0	0.0	0.13914076668125822	0.0
36	0.0	0.0	0.0	0.16728159589769245	0.0
37	0.0	0.0	0.0	0.29235194797073355	0.0
38	0.0	0.0	0.0	0.42992933525107874	0.0
39	0.0	0.0	0.0	0.6237883809642925	0.0
40	0.0	0.0	0.0	0.8489150146957664	0.0
41	0.0	0.0	0.0	1.1756613094865862	0.0
42	0.0	0.0	0.0	1.5555625039084484	0.0
43	0.0	0.0	0.0	1.9026327309111375	0.0
44	0.0	0.0	0.0	2.293477581139391	0.0
45	0.0	0.0	0.0	2.5576886998936903	0.0
46	0.0	0.0	0.0	2.909449065099118	0.0
47	0.0	0.0	0.0	3.350322056156588	0.0
48	0.0	0.0	0.0	3.6551810393346256	0.0
49	0.0	0.0	0.0	3.903758364079795	0.0
50	0.0	0.0	0.0	4.360265149146395	0.0
51	0.0	0.0	0.0	4.848039522231255	0.0
52	0.0	0.0	0.0	5.262335063473204	0.0
53	0.0	0.0	0.0	5.856419235820149	0.0
54	0.0	0.0	0.0	6.366080920517791	0.0
55	0.0	0.0	0.0	6.742855356137827	0.0
56	0.0	0.0	0.0	7.100869238946908	0.0
57	0.0	0.0	0.0	7.4041648427240325	0.0
58	0.0	0.0	0.0	7.712150584703896	0.0
59	0.0	0.0	0.0	8.037333500093803	0.0
60	0.0	0.0	0.0	8.282784066037145	0.0
61	0.0	0.0	0.0	8.52354449377775	0.0
62	0.0	0.0	0.0	8.718966918891876	0.0
63	0.0	0.0	0.0	8.975361140641612	0.0
64	0.0	0.0	0.0	9.230191982990432	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	15	0.0022152828	70.00001	35
CGACTAG	15	0.0022152828	70.00001	22
CCGTATC	30	8.290328E-6	58.333336	55
CGTATCA	25	2.3739437E-4	55.999996	56
CGCCGTA	25	2.3739437E-4	55.999996	53
TCGGTGG	50	9.567884E-10	55.999996	45
GCCGTAT	25	2.3739437E-4	55.999996	54
CGGTGGT	45	2.6499038E-8	54.444443	46
AGTGTAG	45	2.6499038E-8	54.444443	35
CTTGTCA	20	0.006922205	52.5	1
GTACATC	20	0.006922205	52.5	38
CCGATGC	20	0.006922205	52.5	15
CGGCATC	20	0.006922205	52.5	51
TCTTAAC	20	0.006922205	52.5	67
GTATATT	20	0.006922205	52.5	10
GCTCCGG	20	0.006922205	52.5	11
GCCGACT	30	5.837623E-4	46.666668	20
CAGTGTA	30	5.837623E-4	46.666668	34
TCGCCGT	45	1.6607664E-6	46.666664	52
ATCTCGG	45	1.6607664E-6	46.666664	42
>>END_MODULE