FastQCFastQC Report
Wed 25 May 2016
SRR1779757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63964
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT25734.022575198549184No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT5500.8598586705021574RNA PCR Primer, Index 24 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC3210.5018447876930774No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG3200.5002814082921643No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC2740.4283659558501657No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1960.30642236257895067RNA PCR Primer, Index 24 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT1170.1829153899068226RNA PCR Primer, Index 24 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1040.16259145769495342No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1040.16259145769495342No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1010.15790131949221436No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC810.1266337314739541No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG780.12194359327121507No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT770.12038021387030205No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG150.002215282870.0000111
CTCGGGG150.002215282870.0000114
ATTGCTC150.002215282870.0000110
CCCGACG150.002215282870.0000117
GGCATGC200.00692220552.56
ATTGAGA200.00692220552.54
GATTGCT305.837623E-446.6666689
AGCGTCA459.104788E-538.8888853
CAGCGTC501.6925215E-435.02
ATAGCAC400.002402447235.029
TCAGATG501.6925215E-435.07
GCAGCGT400.002402447235.01
CGTCAGA501.6925215E-435.05
GAGGGAT400.002402447235.040
CACCCTT400.002402447235.05
CCACAGG400.002402447235.01
ATCTCGT6150.034.71544637
TCTCGTA6150.034.71544638
CTTGAAA6350.034.1732357
CATCTCG6150.034.1463436