FastQCFastQC Report
Wed 25 May 2016
SRR1779756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33249
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACAAA150.002194005570.09502463
GTACATC150.002207188969.98945638
GCTGCCT207.821771E-569.98945617
TTCCGAC309.950054E-869.98945619
CCCGTGT353.5724952E-969.98945633
ACAGACG150.002207188969.9894568
TTACGCT150.002207188969.98945613
GACGCTT150.002207188969.98945614
GACGAAT150.002207188969.98945623
ATCTCGG352.8912473E-759.99096342
GGTGGTC352.8912473E-759.99096347
CTTCCGA308.210312E-658.32454718
CGACGAA308.210312E-658.32454722
TCCGACG308.210312E-658.32454720
CCGTGTA308.210312E-658.32454734
CGCCGTA308.210312E-658.32454753
GTGGTCG308.210312E-658.32454748
CTGCCTA252.3590148E-455.99156618
TGCCTAC252.3590148E-455.99156619
TCGGTGG509.349606E-1055.99156645
Produced by FastQC (version 0.11.5)