##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779756_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33249
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.630214442539625	34.0	23.0	34.0	12.0	34.0
2	27.33931847574363	33.0	23.0	34.0	12.0	34.0
3	26.574062377815874	32.0	21.0	34.0	11.0	34.0
4	28.605191133567928	33.0	23.0	34.0	12.0	34.0
5	28.203885831152817	34.0	23.0	34.0	11.0	34.0
6	30.62883094228398	36.0	26.0	38.0	11.0	38.0
7	31.579897139763602	37.0	27.0	38.0	12.0	38.0
8	30.855063310174742	37.0	26.0	38.0	11.0	38.0
9	32.23540557610755	37.0	28.0	38.0	12.0	38.0
10-11	31.86682306234774	37.0	28.0	38.0	11.0	38.0
12-13	31.048227014346296	36.5	27.5	38.0	11.0	38.0
14-15	30.91462901139884	36.5	26.5	38.0	11.0	38.0
16-17	30.663072573611238	36.0	25.5	38.0	11.0	38.0
18-19	31.17287737977082	37.0	26.5	38.0	11.5	38.0
20-21	30.18371981112214	35.5	24.5	38.0	11.0	38.0
22-23	30.221510421366055	35.5	24.5	38.0	11.0	38.0
24-25	31.343183253631686	36.5	27.0	38.0	11.0	38.0
26-27	29.843905079852025	35.5	24.0	38.0	11.0	38.0
28-29	31.806625763180847	37.0	29.0	38.0	11.0	38.0
30-31	32.31077626394779	38.0	31.0	38.0	11.0	38.0
32-33	32.38103401606063	38.0	31.0	38.0	11.0	38.0
34-35	32.43413335739421	38.0	31.5	38.0	11.0	38.0
36-37	30.45315648590935	36.5	25.0	38.0	11.0	38.0
38-39	29.114349303738457	34.0	23.0	37.5	11.0	38.0
40-41	30.032376913591385	35.0	24.5	38.0	11.0	38.0
42-43	31.022647297663088	36.5	25.5	38.0	11.0	38.0
44-45	32.455502421125445	37.5	31.5	38.0	11.0	38.0
46-47	31.155688892899033	37.0	28.0	38.0	11.0	38.0
48-49	30.39178621913441	35.5	24.0	38.0	11.0	38.0
50-51	28.544632921290866	34.0	21.5	38.0	11.0	38.0
52-53	31.62944750218052	37.0	28.5	38.0	11.0	38.0
54-55	30.74295467532858	36.5	25.5	38.0	11.0	38.0
56-57	31.175478961773287	37.0	27.0	38.0	11.0	38.0
58-59	32.19747962344732	37.5	31.5	38.0	11.0	38.0
60-61	32.66650124815784	38.0	34.0	38.0	11.0	38.0
62-63	32.555309934133355	38.0	33.0	38.0	11.0	38.0
64-65	32.278639959096516	38.0	30.5	38.0	11.0	38.0
66-67	30.6659899545851	37.0	24.5	38.0	11.0	38.0
68-69	30.515308731089654	36.5	24.5	38.0	11.0	38.0
70-71	30.088168065204968	36.5	24.0	38.0	11.0	38.0
72-73	29.090950103762516	34.0	22.0	38.0	11.0	38.0
74-75	30.794038918463713	36.5	25.5	38.0	11.0	38.0
76	31.37324430809949	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	18.0
13	123.0
14	240.0
15	464.0
16	692.0
17	972.0
18	1127.0
19	1167.0
20	985.0
21	737.0
22	543.0
23	438.0
24	385.0
25	361.0
26	386.0
27	465.0
28	489.0
29	631.0
30	810.0
31	1102.0
32	1498.0
33	2370.0
34	3534.0
35	4803.0
36	5770.0
37	3137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.997954825709048	21.03221149508256	14.902703840717013	32.06712983849138
2	18.03663268068213	16.144846461547715	43.85695810400313	21.96156275376703
3	17.93437396613432	18.602063219946466	38.449276669975035	25.01428614394418
4	12.791362146229963	21.829227946705167	33.07768654696382	32.30172336010105
5	12.28909140124515	29.57682937832717	40.97266083190472	17.161418388522964
6	24.773677403831694	35.017594514120724	16.93584769466751	23.27288038738007
7	23.435291286955998	25.83235586032663	22.1961562753767	28.53619657734067
8	19.7118710337153	34.30479112153749	18.593040392192247	27.39029745255496
9	21.32094198321754	16.039580137748505	20.280309182231044	42.35916869680291
10-11	22.653653548280374	24.73495044889243	27.566657142427474	25.04473886039972
12-13	21.889380131733287	20.384071701404554	30.130229480585886	27.596318686276277
14-15	22.590153087310895	23.271376582754367	23.45634455171584	30.682125778218893
16-17	24.92029595765159	22.37878970163619	25.697786333012512	27.00312800769971
18-19	20.179857737943067	22.877723807089044	28.61181707445449	28.33060138051341
20-21	24.152606093416342	22.296911185298807	26.66847123221751	26.88201148906734
22-23	25.49249601491774	21.30590393696051	26.244398327769257	26.95720172035249
24-25	21.06830280609943	25.528587325934616	23.67890763631989	29.724202231646064
26-27	23.304460284519834	24.188697404433217	26.620349484195017	25.886492826851935
28-29	21.875845890101957	25.122560076994798	26.01281241541099	26.988781617492258
30-31	21.25359038753628	24.942478608057506	26.31246522399507	27.491465780411144
32-33	21.597642034346897	24.764654576077476	25.9676982766399	27.670005112935726
34-35	21.94652470751	24.25185719871274	26.08800264669614	27.713615447081114
36-37	24.378910009624637	21.735141963426372	29.839388835418674	24.046559191530317
38-39	24.431544754571703	20.20271896053898	33.40050529355149	21.965230991337826
40-41	22.47169045220085	24.017624855256628	25.19512158443238	28.31556310811014
42-43	19.29080573851845	24.75563174832326	27.127131643056934	28.826430870101355
44-45	19.800893273380755	23.186008391356	29.022361911063655	27.990736424199586
46-47	23.997714216968934	21.69238172576619	27.901591025293992	26.408313031970888
48-49	23.545423101794068	22.61154638555123	28.942659067326343	24.90037144532836
50-51	27.573761616890735	20.484826611326657	28.817408042347136	23.124003729435472
52-53	19.630960795223846	23.599560882445825	28.563694602764034	28.205783719566295
54-55	22.936028151222594	22.57210743180246	27.63391380191885	26.85795061505609
56-57	23.011218382507746	22.787151493277992	26.50004511413877	27.70158501007549
58-59	19.731717220325727	24.39658931981894	28.17570717475976	27.695986285095568
60-61	19.57381536009143	23.157435673789795	28.086981367580492	29.18176759853828
62-63	19.429447945020076	24.817660947110394	27.25837255816052	28.494518549709007
64-65	19.908869439682398	25.746638996661552	26.910583776955697	27.433907786700352
66-67	18.527430221366696	26.995608758421557	23.15176852743022	31.32519249278152
68-69	18.55701114503587	27.41588581226405	23.500834749650306	30.526268293049768
70-71	19.319959095283927	27.339990375360923	22.669032723772858	30.67101780558229
72-73	18.852261790182865	27.057266602502406	21.998315688161693	32.09215591915303
74-75	20.41776948989413	26.05720644850818	24.74584937439846	28.779174687199234
76	21.883364913230473	25.06842311046949	25.31203945983338	27.736172516466663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	1.5
17	2.0
18	3.5
19	5.5
20	8.5
21	11.0
22	13.0
23	18.0
24	29.5
25	38.0
26	51.5
27	92.5
28	126.5
29	133.0
30	171.5
31	256.0
32	358.0
33	414.0
34	446.5
35	602.5
36	861.0
37	996.0
38	1074.5
39	1296.0
40	1488.5
41	1666.5
42	1795.0
43	1856.5
44	1947.5
45	1993.5
46	2010.0
47	2006.5
48	1924.5
49	1798.0
50	1750.0
51	1614.5
52	1399.0
53	1242.0
54	1165.0
55	1037.5
56	812.5
57	691.0
58	667.0
59	630.0
60	565.0
61	527.5
62	518.0
63	479.0
64	444.0
65	442.0
66	446.0
67	456.0
68	435.5
69	384.0
70	363.0
71	373.0
72	360.5
73	312.0
74	245.0
75	214.0
76	198.0
77	163.5
78	120.0
79	95.0
80	79.0
81	59.0
82	45.0
83	35.0
84	27.5
85	15.5
86	7.5
87	4.0
88	2.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0015038046257030285
12-13	0.0
14-15	0.0
16-17	0.003007609251406057
18-19	0.0015038046257030285
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0015038046257030285
32-33	0.0
34-35	0.0
36-37	0.003007609251406057
38-39	0.003007609251406057
40-41	0.0015038046257030285
42-43	0.0
44-45	0.0015038046257030285
46-47	0.0
48-49	0.0015038046257030285
50-51	0.0
52-53	0.0015038046257030285
54-55	0.0
56-57	0.0
58-59	0.0015038046257030285
60-61	0.0015038046257030285
62-63	0.0015038046257030285
64-65	0.0
66-67	0.003007609251406057
68-69	0.016541850882733314
70-71	0.003007609251406057
72-73	0.003007609251406057
74-75	0.003007609251406057
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	33249.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.6209810821378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.9696838991928	95.63896658546122
2	1.7591965000924272	3.4346897651057176
3	0.18177336866103888	0.5323468374988721
4	0.05853718651796168	0.22857830310686034
5	0.015404522767884652	0.07519023128515144
6	0.015404522767884652	0.09022827754218171
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.006015218502812115	0.0
5	0.0	0.0	0.0	0.006015218502812115	0.0
6	0.0	0.0	0.0	0.006015218502812115	0.0
7	0.0	0.0	0.0	0.006015218502812115	0.0
8	0.0	0.0	0.0	0.006015218502812115	0.0
9	0.0	0.0	0.0	0.006015218502812115	0.0
10	0.0	0.0	0.0	0.006015218502812115	0.0
11	0.0	0.0	0.0	0.006015218502812115	0.0
12	0.0	0.0	0.0	0.006015218502812115	0.0
13	0.0	0.0	0.0	0.006015218502812115	0.0
14	0.0	0.0	0.0	0.009022827754218171	0.0
15	0.0	0.0	0.0	0.009022827754218171	0.0
16	0.0	0.0	0.0	0.009022827754218171	0.0
17	0.0	0.0	0.0	0.009022827754218171	0.0
18	0.0	0.0	0.0	0.009022827754218171	0.0
19	0.0	0.0	0.0	0.01203043700562423	0.0
20	0.0	0.0	0.0	0.015038046257030287	0.0
21	0.0	0.0	0.0	0.018045655508436342	0.0
22	0.0	0.0	0.0	0.04812174802249692	0.0
23	0.0	0.0	0.0	0.04812174802249692	0.0
24	0.0	0.0	0.0	0.07218262203374537	0.0
25	0.0	0.0	0.0	0.07218262203374537	0.0
26	0.0	0.0	0.0	0.07218262203374537	0.0
27	0.0	0.0	0.0	0.09022827754218173	0.0
28	0.0	0.0	0.0	0.09323588679358778	0.0
29	0.0	0.0	0.0	0.10225871454780595	0.0
30	0.0	0.0	0.0	0.10526632379921201	0.0
31	0.0	0.0	0.0	0.11128154230202413	0.0
32	0.0	0.0	0.0	0.12331197930764835	0.0
33	0.0	0.0	0.0	0.14135763481608468	0.0
34	0.0	0.0	0.0	0.1774489458329574	0.0
35	0.0	0.0	0.0	0.21654786610123614	0.0
36	0.0	0.0	0.0	0.24963156786670276	0.0
37	0.0	0.0	0.0	0.3819663749285693	0.0
38	0.0	0.0	0.0	0.4872326987277813	0.0
39	0.0	0.0	0.0	0.6496435983037084	0.0
40	0.0	0.0	0.0	0.8481458088965081	0.0
41	0.0	0.0	0.0	1.0737165027519624	0.0
42	0.0	0.0	0.0	1.311317633613041	0.0
43	0.0	0.0	0.0	1.5910252939938043	0.0
44	0.0	0.0	0.0	1.9038166561400343	0.0
45	0.0	0.0	0.0	2.093296038978616	0.0
46	0.0	0.0	0.0	2.315859123582664	0.0
47	0.0	0.0	0.0	2.6346657042317063	0.0
48	0.0	0.0	0.0	2.896327709104033	0.0
49	0.0	0.0	0.0	3.085807091942615	0.0
50	0.0	0.0	0.0	3.362507143071972	0.0
51	0.0	0.0	0.0	3.633191975698517	0.0
52	0.0	0.0	0.0	3.9519985563475593	0.0
53	0.0	0.0	0.0	4.285843183253632	0.0
54	0.0	0.0	0.0	4.610664982405486	0.0
55	0.0	0.0	0.0	4.809167192998285	0.0
56	0.0	0.0	0.0	5.079852025624831	0.0
57	0.0	0.0	0.0	5.272339017714819	0.0
58	0.0	0.0	0.0	5.4618184005534	0.0
59	0.0	0.0	0.0	5.6903967036602605	0.0
60	0.0	0.0	0.0	5.8528076032361875	0.0
61	0.0	0.0	0.0	6.003188065806491	0.0
62	0.0	0.0	0.0	6.147553309873981	0.0
63	0.0	0.0	0.0	6.328009864958345	0.0
64	0.0	0.0	0.0	6.469367499774429	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACAAA	15	0.0021940055	70.095024	63
GTACATC	15	0.0022071889	69.989456	38
GCTGCCT	20	7.821771E-5	69.989456	17
TTCCGAC	30	9.950054E-8	69.989456	19
CCCGTGT	35	3.5724952E-9	69.989456	33
ACAGACG	15	0.0022071889	69.989456	8
TTACGCT	15	0.0022071889	69.989456	13
GACGCTT	15	0.0022071889	69.989456	14
GACGAAT	15	0.0022071889	69.989456	23
ATCTCGG	35	2.8912473E-7	59.990963	42
GGTGGTC	35	2.8912473E-7	59.990963	47
CTTCCGA	30	8.210312E-6	58.324547	18
CGACGAA	30	8.210312E-6	58.324547	22
TCCGACG	30	8.210312E-6	58.324547	20
CCGTGTA	30	8.210312E-6	58.324547	34
CGCCGTA	30	8.210312E-6	58.324547	53
GTGGTCG	30	8.210312E-6	58.324547	48
CTGCCTA	25	2.3590148E-4	55.991566	18
TGCCTAC	25	2.3590148E-4	55.991566	19
TCGGTGG	50	9.349606E-10	55.991566	45
>>END_MODULE