FastQCFastQC Report
Wed 25 May 2016
SRR1779756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33249
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT28418.544617883244609No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT6571.9759992781737796TruSeq Adapter, Index 12 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG4221.269211104093356No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3881.1669523895455503TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3030.9113056031760355RNA PCR Primer, Index 12 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3010.9052903846732232No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1440.43309573220247227No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1390.418057685945442RNA PCR Primer, Index 12 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1250.37595115642575716TruSeq Adapter, Index 12 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1120.3368522361574784No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG900.2706848326265452No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC810.24361634936389065No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT650.19549460134139374No Hit
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT610.1834641643357695No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT410.12331197930764835No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT400.1203043700562423No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTAT150.00220587170.048
CCATCTA150.00220587170.013
CGAGACA207.8159406E-570.023
CTATACA750.060.6666681
TCTTTAT352.8882278E-760.01
CTTAATA252.3572598E-456.0000042
TTAATAC252.3572598E-456.0000043
CTTTATT200.006893018352.549
CTTTATA407.264607E-752.52
CCCACCC200.006893018352.53
GCGTCAG200.006893018352.51
AATACAC305.796844E-446.6666645
TTTACAC305.796844E-446.6666642
TTTATAC451.6365011E-646.6666643
TTCTGCT7550.041.25827852
TGCTTGA7550.041.25827855
ATCTCGT7150.041.1188837
TCTCGTA7150.041.1188838
CTGCTTG7500.041.0666754
TCGTATG7700.040.9090940
Produced by FastQC (version 0.11.5)