##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779756_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33249
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.27017353905381	34.0	34.0	34.0	32.0	34.0
2	33.29252007579176	34.0	34.0	34.0	33.0	34.0
3	33.3664170350988	34.0	34.0	34.0	33.0	34.0
4	33.42286986074769	34.0	34.0	34.0	34.0	34.0
5	33.378988841769676	34.0	34.0	34.0	34.0	34.0
6	36.62534211555235	38.0	37.0	38.0	34.0	38.0
7	36.7809558182201	38.0	37.0	38.0	34.0	38.0
8	36.40533549881199	38.0	37.0	38.0	34.0	38.0
9	36.503804625703026	38.0	37.0	38.0	34.0	38.0
10-11	36.380838521459296	38.0	37.0	38.0	34.0	38.0
12-13	36.37104875334596	38.0	37.0	38.0	34.0	38.0
14-15	36.409741646365305	38.0	37.0	38.0	34.0	38.0
16-17	36.21202141417787	38.0	37.0	38.0	33.5	38.0
18-19	36.22345032933321	38.0	37.0	38.0	34.0	38.0
20-21	36.35434148395441	38.0	37.0	38.0	34.0	38.0
22-23	36.219390056843814	38.0	37.0	38.0	34.0	38.0
24-25	36.14901200036091	38.0	36.0	38.0	33.5	38.0
26-27	35.98261601852687	38.0	36.0	38.0	32.0	38.0
28-29	35.79435471743511	38.0	36.0	38.0	31.5	38.0
30-31	35.584829618935906	38.0	36.0	38.0	31.0	38.0
32-33	35.572107431802465	38.0	36.0	38.0	31.0	38.0
34-35	35.28979818941923	38.0	36.0	38.0	29.0	38.0
36-37	35.45196848025505	38.0	36.0	38.0	31.0	38.0
38-39	35.05209179223435	38.0	35.5	38.0	27.0	38.0
40-41	35.65072633763421	38.0	36.0	38.0	31.0	38.0
42-43	35.88250774459382	38.0	36.0	38.0	32.0	38.0
44-45	35.88201148906734	38.0	36.0	38.0	32.0	38.0
46-47	35.87076303046708	38.0	36.0	38.0	32.0	38.0
48-49	35.83262654515926	38.0	36.0	38.0	31.5	38.0
50-51	35.74636831182893	38.0	36.0	38.0	31.0	38.0
52-53	35.809076964720745	38.0	36.0	38.0	31.0	38.0
54-55	35.76746669072754	38.0	36.0	38.0	31.0	38.0
56-57	35.72899936840206	38.0	36.0	38.0	31.0	38.0
58-59	35.62113146260038	38.0	36.0	38.0	31.0	38.0
60-61	35.62726698547325	38.0	36.0	38.0	31.0	38.0
62-63	35.68376492526092	38.0	36.0	38.0	31.0	38.0
64-65	35.695810400312794	38.0	36.0	38.0	31.0	38.0
66-67	35.62436464254564	38.0	36.0	38.0	31.0	38.0
68-69	35.170065265120755	38.0	36.0	38.0	28.0	38.0
70-71	33.91505007669404	37.5	34.5	38.0	23.0	38.0
72-73	32.22059310054438	37.0	30.0	38.0	11.0	38.0
74-75	31.932449096213418	37.0	28.0	38.0	11.0	38.0
76	31.251586513880117	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	4.0
22	4.0
23	14.0
24	22.0
25	44.0
26	99.0
27	165.0
28	328.0
29	454.0
30	719.0
31	1176.0
32	1639.0
33	2553.0
34	3800.0
35	5585.0
36	6532.0
37	10109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.570934464194416	19.041174170651747	13.759812325182713	33.62807903997113
2	18.196035971006648	17.441126048903726	42.554663298144305	21.80817468194532
3	17.089235766489217	22.857830310686037	39.09892026827875	20.954013654546003
4	13.230473096935247	26.79178321152516	34.54239225239857	25.435351439141023
5	12.80038497398418	35.736413125206774	38.74101476736143	12.722187133447624
6	25.41730578363259	41.706517489247794	16.67719329904659	16.198983428073024
7	23.36611627417366	29.826460946193873	19.784053655749045	27.023369123883423
8	20.38256789677885	41.387710908598756	17.579476074468406	20.65024512015399
9	21.071310415350837	18.538903425666938	17.173448825528588	43.21633733345364
10-11	22.33450630094138	27.122620229179823	28.456494932178412	22.086378537700384
12-13	22.475863935757467	18.373484916839605	29.03545971307408	30.115191434328853
14-15	22.585641673433788	19.59758188216187	20.00511293572739	37.81166350867695
16-17	28.960269481788924	27.460976269963005	18.863725224818793	24.715029023429278
18-19	27.781286655237754	23.370627688050767	19.794580288128966	29.053505368582517
20-21	21.990135041655385	21.68787031188908	19.430659568708833	36.8913350777467
22-23	23.823272880387382	28.21137477818882	19.123883425065415	28.841468916358387
24-25	28.915155343017833	29.283587476315077	19.734428103100846	22.06682907756624
26-27	27.882041565159852	30.418959968720866	21.438238744022375	20.260759722096903
28-29	21.0036392071942	23.672892417817078	28.727179764804955	26.596288610183766
30-31	21.054768564468105	26.262443983277695	25.794760744684055	26.888026707570152
32-33	21.414177870011127	23.860867996029956	33.42656922012692	21.298384913831995
34-35	27.187283828085057	29.793377244428402	24.865409485999578	18.153929441486962
36-37	21.929982856627266	35.98754849769918	22.087882342326086	19.994586303347468
38-39	20.720923937562034	23.83831092664441	28.615898222502935	26.824866913290624
40-41	21.426208307016754	20.46678095581822	29.939246293121595	28.16776444404343
42-43	27.851965472645794	20.769045685584526	30.261060483022046	21.11792835874763
44-45	21.923967638124456	26.462449998496197	30.429486601100784	21.184095762278567
46-47	28.205359559686006	20.132936328912148	24.054858792745645	27.606845318656198
48-49	27.682035549941354	18.80357303979067	23.812746248007457	29.70164516226052
50-51	21.442750157899486	19.451712833468676	30.643026857950616	28.462510150681226
52-53	19.874281933291225	19.271256278384314	38.159042377214355	22.69541941111011
54-55	20.254744503594093	19.63818460705585	32.77542181719751	27.331649072152548
56-57	26.80832506240789	19.391560648440553	26.418839664350806	27.381274624800746
58-59	21.46079581340792	22.157057355108424	35.03413636500346	21.348010466480194
60-61	25.892508045354745	30.56482901741406	24.00824084934885	19.53442208788234
62-63	18.707329543745676	40.89897440524527	20.681825017293754	19.7118710337153
64-65	18.56597190892959	42.283978465517755	19.60810851454179	19.541941111010857
66-67	18.735901831634035	41.37417666696743	19.857740082408494	20.032181418990046
68-69	18.40205720472796	41.16514782399471	19.98105206171614	20.45174290956119
70-71	18.86222142019309	38.59364191404253	20.997623988691387	21.546512677072997
72-73	19.217119311859005	34.28674546602905	21.859304039219225	24.636831182892717
74-75	19.19456224247346	32.85963487623688	23.39168095281061	24.554121928479052
76	19.30584378477548	32.49421035219105	23.66386959006286	24.536076272970618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.5
16	3.0
17	3.0
18	4.5
19	6.5
20	7.0
21	7.0
22	14.5
23	24.5
24	38.0
25	49.0
26	75.0
27	97.0
28	120.5
29	148.0
30	174.0
31	230.5
32	348.5
33	436.0
34	483.5
35	583.0
36	801.5
37	968.0
38	1782.0
39	2975.0
40	3180.5
41	2666.0
42	2325.0
43	2128.0
44	1866.0
45	1707.5
46	1614.0
47	1601.5
48	1452.0
49	1239.5
50	1164.0
51	1072.0
52	873.5
53	756.5
54	746.0
55	661.5
56	527.5
57	430.0
58	382.0
59	340.0
60	309.5
61	317.0
62	313.0
63	310.5
64	319.0
65	330.0
66	330.0
67	330.0
68	352.5
69	375.5
70	365.5
71	355.0
72	342.5
73	313.0
74	304.5
75	313.0
76	287.0
77	223.5
78	170.0
79	154.0
80	122.5
81	83.0
82	57.5
83	40.0
84	31.0
85	17.5
86	8.0
87	3.0
88	4.0
89	3.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	33249.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.353454239225236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0116619750675	37.17705795663028
2	16.125696558855633	16.884718337393608
3	6.629516861032918	10.41234322836777
4	2.8092146837479173	5.882883695750248
5	1.338541965875797	3.503864777888057
6	0.7295915436318723	2.2917982495714155
7	0.37915781007640603	1.3895154741495985
8	0.2700063192968346	1.1308610785286775
9	0.17234445912563912	0.8120544978796355
>10	0.4538404090308497	3.2843093025354144
>50	0.02297926121675188	0.893259947667599
>100	0.04595852243350376	5.816716292219315
>500	0.00574481530418797	1.9759992781737796
>1k	0.00574481530418797	8.544617883244609
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	2841	8.544617883244609	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	657	1.9759992781737796	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	422	1.269211104093356	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	388	1.1669523895455503	TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	303	0.9113056031760355	RNA PCR Primer, Index 12 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	301	0.9052903846732232	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	144	0.43309573220247227	No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT	139	0.418057685945442	RNA PCR Primer, Index 12 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	125	0.37595115642575716	TruSeq Adapter, Index 12 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	112	0.3368522361574784	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	90	0.2706848326265452	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	81	0.24361634936389065	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	65	0.19549460134139374	No Hit
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	61	0.1834641643357695	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT	41	0.12331197930764835	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	40	0.1203043700562423	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.009022827754218171	0.0
5	0.0	0.0	0.0	0.009022827754218171	0.0
6	0.0	0.0	0.0	0.009022827754218171	0.0
7	0.0	0.0	0.0	0.009022827754218171	0.0
8	0.0	0.0	0.0	0.009022827754218171	0.0
9	0.0	0.0	0.0	0.009022827754218171	0.0
10	0.0	0.0	0.0	0.009022827754218171	0.0
11	0.0	0.0	0.0	0.009022827754218171	0.0
12	0.0	0.0	0.0	0.009022827754218171	0.0
13	0.0	0.0	0.0	0.009022827754218171	0.0
14	0.0	0.0	0.0	0.01203043700562423	0.0
15	0.0	0.0	0.0	0.01203043700562423	0.0
16	0.0	0.0	0.0	0.01203043700562423	0.0
17	0.0	0.0	0.0	0.01203043700562423	0.0
18	0.0	0.0	0.0	0.01203043700562423	0.0
19	0.0	0.0	0.0	0.02406087401124846	0.0
20	0.0	0.0	0.0	0.027068483262654517	0.0
21	0.0	0.0	0.0	0.030076092514060574	0.0
22	0.0	0.0	0.0	0.030076092514060574	0.0
23	0.0	0.0	0.0	0.030076092514060574	0.0
24	0.0	0.0	0.0	0.030076092514060574	0.0
25	0.0	0.0	0.0	0.030076092514060574	0.0
26	0.0	0.0	0.0	0.030076092514060574	0.0
27	0.0	0.0	0.0	0.05112935727390298	0.0
28	0.0	0.0	0.0	0.05714457577671509	0.0
29	0.0	0.0	0.0	0.06917501278233931	0.0
30	0.0	0.0	0.0	0.07819784053655748	0.0
31	0.0	0.0	0.0	0.08722066829077567	0.0
32	0.0	0.0	0.0	0.10526632379921201	0.0
33	0.0	0.0	0.0	0.1323348070618665	0.0
34	0.0	0.0	0.0	0.1985022105927998	0.0
35	0.0	0.0	0.0	0.27970766038076333	0.0
36	0.0	0.0	0.0	0.3879815934313814	0.0
37	0.0	0.0	0.0	0.6225751150410539	0.0
38	0.0	0.0	0.0	0.781978405365575	0.0
39	0.0	0.0	0.0	1.0135643177238414	0.0
40	0.0	0.0	0.0	1.2692111040933562	0.0
41	0.0	0.0	0.0	1.530873108965683	0.0
42	0.0	0.0	0.0	1.8226112063520707	0.0
43	0.0	0.0	0.0	2.1684862702637675	0.0
44	0.0	0.0	0.0	2.5594754729465548	0.0
45	0.0	0.0	0.0	2.947457066377936	0.0
46	0.0	0.0	0.0	3.305362567295257	0.0
47	0.0	0.0	0.0	3.720412643989293	0.0
48	0.0	0.0	0.0	4.117417065174893	0.0
49	0.0	0.0	0.0	4.469307347589401	0.0
50	0.0	0.0	0.0	4.7881139282384435	0.0
51	0.0	0.0	0.0	5.097897681133267	0.0
52	0.0	0.0	0.0	5.485879274564649	0.0
53	0.0	0.0	0.0	5.84378477548197	0.0
54	0.0	0.0	0.0	6.17161418388523	0.0
55	0.0	0.0	0.0	6.418238142500527	0.0
56	0.0	0.0	0.0	6.770128424915035	0.0
57	0.0	0.0	0.0	7.100965442569701	0.0
58	0.0	0.0	0.0	7.392703539956089	0.0
59	0.0	0.0	0.0	7.687449246593882	0.0
60	0.0	0.0	0.0	7.997232999488706	0.0
61	0.0	0.0	0.0	8.294986315377907	0.0
62	0.0	0.0	0.0	8.664922253300851	0.0
63	0.0	0.0	0.0	9.055911455983638	0.0
64	0.0	0.0	0.0	9.389756082889711	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTTAT	15	0.002205871	70.0	48
CCATCTA	15	0.002205871	70.0	13
CGAGACA	20	7.8159406E-5	70.0	23
CTATACA	75	0.0	60.666668	1
TCTTTAT	35	2.8882278E-7	60.0	1
CTTAATA	25	2.3572598E-4	56.000004	2
TTAATAC	25	2.3572598E-4	56.000004	3
CTTTATT	20	0.0068930183	52.5	49
CTTTATA	40	7.264607E-7	52.5	2
CCCACCC	20	0.0068930183	52.5	3
GCGTCAG	20	0.0068930183	52.5	1
AATACAC	30	5.796844E-4	46.666664	5
TTTACAC	30	5.796844E-4	46.666664	2
TTTATAC	45	1.6365011E-6	46.666664	3
TTCTGCT	755	0.0	41.258278	52
TGCTTGA	755	0.0	41.258278	55
ATCTCGT	715	0.0	41.11888	37
TCTCGTA	715	0.0	41.11888	38
CTGCTTG	750	0.0	41.06667	54
TCGTATG	770	0.0	40.90909	40
>>END_MODULE