##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779755_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130360
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.710072108008593	34.0	31.0	34.0	12.0	34.0
2	27.936422215403496	34.0	23.0	34.0	12.0	34.0
3	26.82583614605707	32.0	21.0	34.0	11.0	34.0
4	29.489659404725376	34.0	27.0	34.0	12.0	34.0
5	28.886905492482356	34.0	23.0	34.0	11.0	34.0
6	31.35449524393986	37.0	27.0	38.0	11.0	38.0
7	32.46133783369132	37.0	31.0	38.0	12.0	38.0
8	31.670251610923597	37.0	27.0	38.0	11.0	38.0
9	33.190104326480515	37.0	34.0	38.0	21.0	38.0
10-11	32.93040810064437	37.0	33.5	38.0	11.5	38.0
12-13	31.837914237496165	37.0	29.5	38.0	16.0	38.0
14-15	31.963297790733353	36.5	29.0	38.0	11.5	38.0
16-17	31.619254372506905	36.5	29.0	38.0	16.0	38.0
18-19	32.11409558146671	37.0	29.5	38.0	11.5	38.0
20-21	31.08466937710954	35.5	27.0	38.0	16.0	38.0
22-23	31.063293955200983	35.5	27.5	38.0	11.0	38.0
24-25	32.36584841976066	37.0	30.5	38.0	16.5	38.0
26-27	30.824892605093588	36.5	25.0	38.0	11.0	38.0
28-29	33.077462411782754	37.0	33.5	38.0	21.0	38.0
30-31	33.57786897821418	38.0	34.0	38.0	21.0	38.0
32-33	33.6621049401657	38.0	34.0	38.0	21.0	38.0
34-35	33.73547100337527	38.0	34.0	38.0	21.0	38.0
36-37	31.409703896900886	36.5	27.5	38.0	11.0	38.0
38-39	29.515702669530533	34.5	23.0	37.5	11.0	38.0
40-41	30.861640840748695	35.0	26.5	38.0	11.0	38.0
42-43	32.02565971156797	36.5	30.0	38.0	16.0	38.0
44-45	33.78085685793188	37.5	34.0	38.0	21.0	38.0
46-47	32.215207885854554	37.0	29.5	38.0	16.0	38.0
48-49	31.538984351027924	36.5	28.5	38.0	16.0	38.0
50-51	29.276338600797793	34.5	22.5	38.0	11.0	38.0
52-53	32.9170029150046	37.0	32.5	38.0	21.0	38.0
54-55	31.916254986192087	37.0	29.5	38.0	16.0	38.0
56-57	32.330584535133475	37.0	30.0	38.0	16.0	38.0
58-59	33.54899125498619	37.5	34.0	38.0	21.0	38.0
60-61	34.02272552930347	38.0	35.0	38.0	21.0	38.0
62-63	33.9045029150046	38.0	34.5	38.0	21.0	38.0
64-65	33.556190549248235	38.0	34.5	38.0	16.0	38.0
66-67	31.398362227677204	37.0	27.0	38.0	11.0	38.0
68-69	31.472177048174288	37.0	27.5	38.0	11.0	38.0
70-71	30.908376802700214	36.5	25.5	38.0	11.0	38.0
72-73	29.583127493096043	34.5	23.5	38.0	11.0	38.0
74-75	31.855695765572264	36.5	29.0	38.0	16.0	38.0
76	32.42377262964099	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	28.0
13	188.0
14	514.0
15	852.0
16	1380.0
17	2121.0
18	2733.0
19	2897.0
20	2550.0
21	2118.0
22	1681.0
23	1502.0
24	1554.0
25	1719.0
26	1984.0
27	2370.0
28	2879.0
29	3560.0
30	4549.0
31	6071.0
32	8269.0
33	11375.0
34	15164.0
35	19752.0
36	21343.0
37	11206.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.35747161706045	18.02623504142375	11.0632095734888	30.553083768027
2	21.27262964099417	18.01319423135931	37.21540349800552	23.498772629640992
3	20.287664927891992	17.270635164160787	35.48327707885854	26.95842282908868
4	14.701595581466709	18.521785823872353	33.103712795335994	33.67290579932495
5	14.568119054924825	28.59849647131022	39.305768640687326	17.527615833077633
6	27.46624731512734	32.65265418840135	17.791500460263883	22.089598036207427
7	27.94645596808837	25.928198833998156	20.729518257134092	25.395826940779383
8	23.79582537454261	31.05884518905484	19.025920726608827	26.119408709793724
9	23.390790049018477	16.339493245575678	20.333080186254882	39.93663651915096
10-11	25.07728657016393	23.326352610866913	26.971670540584082	24.624690278385074
12-13	24.6520173214635	19.77584890897026	28.955150603911523	26.616983165654712
14-15	23.600595279190543	22.33716122400448	24.203545593323053	29.858697903481925
16-17	24.865754307368938	23.028889673054202	26.102348916061924	26.003007103514935
18-19	22.380205356766147	22.914884721748102	27.845518320631186	26.859391600854565
20-21	24.120988497194297	22.65734373022296	27.580651966293214	25.64101580628953
22-23	24.918494311861856	22.029549168066648	27.780590523093917	25.27136599697758
24-25	22.826962465192278	24.865947115273972	24.266448806756728	28.040641612777023
26-27	23.268435627766397	23.65774514993211	27.476430472771344	25.597388749530143
28-29	23.422241655735316	24.732086008637687	26.106751355871094	25.738920979755903
30-31	23.277730259246614	24.240843519985273	26.256438974059996	26.224987246708114
32-33	23.088164223413806	24.487760722313	26.37945979947683	26.044615254796373
34-35	23.412652751248476	24.205079818040947	26.41896608596261	25.963301344747965
36-37	24.02864397548309	22.29650426898028	29.85332811696929	23.821523638567342
38-39	23.933230284177863	21.24909384505038	32.85976748735219	21.957908383419568
40-41	23.15652604164669	23.190279114902363	25.63124000352873	28.021954839922213
42-43	21.161561533917872	23.368927346788485	27.132380579783522	28.337130539510124
44-45	21.201067820403654	22.327955875697114	29.460566589188318	27.010409714710914
46-47	23.02295967290329	21.120904578893672	29.995627459554004	25.860508288649037
48-49	22.900221695471735	21.71081398292408	30.472387790639694	24.91657653096449
50-51	24.4358869127298	20.49179384701537	31.92057349099989	23.151745749254946
52-53	19.58706341717871	23.821523638567342	29.52423691498094	27.06717602927301
54-55	21.53054257857148	22.74104588099019	28.930108392976315	26.79830314746201
56-57	21.522487898802538	23.026411678518553	28.27307665753803	27.17802376514088
58-59	19.632014789983007	24.03909219575248	28.74342678076228	27.585466233502228
60-61	19.9699290801904	23.225949976411204	28.43351219905108	28.37060874434732
62-63	20.07464031881312	23.99728441183352	27.75845073393756	28.169624535415792
64-65	20.73082794436901	24.0808076158915	26.514088018472066	28.674276421267425
66-67	19.76134951441415	24.3678945672686	23.479571641172768	32.39118427714448
68-69	20.29216793518291	24.474818929535967	23.508854038792045	31.724159096489075
70-71	21.153587990042844	24.396567926142136	22.969349908137943	31.480494175677077
72-73	21.27816261247785	24.35428317185618	21.52824124149464	32.83931297417133
74-75	23.422625211914788	24.469350025698265	23.211669313204304	28.896355449182643
76	24.750303011706222	23.311956304944843	23.977047822151306	27.96069286119762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	5.0
16	4.5
17	1.0
18	5.0
19	9.0
20	20.0
21	31.0
22	34.5
23	69.5
24	126.5
25	152.0
26	205.5
27	320.5
28	454.0
29	526.0
30	661.5
31	895.5
32	1227.0
33	1460.0
34	1760.5
35	2370.0
36	3020.5
37	3362.0
38	3862.0
39	4711.5
40	5424.0
41	6210.0
42	6633.0
43	6858.0
44	7349.5
45	7650.5
46	7685.0
47	7677.0
48	7489.5
49	7051.5
50	6793.0
51	6322.0
52	5539.0
53	4851.5
54	4476.0
55	4159.5
56	3556.5
57	3049.5
58	2829.0
59	2708.5
60	2461.5
61	2222.0
62	2109.0
63	2110.0
64	2121.0
65	2085.0
66	1969.0
67	1899.0
68	1836.5
69	1704.0
70	1541.0
71	1448.0
72	1389.0
73	1232.5
74	1035.0
75	935.0
76	807.5
77	645.5
78	525.0
79	439.0
80	367.5
81	245.0
82	156.5
83	119.0
84	90.0
85	60.5
86	49.5
87	39.0
88	24.5
89	8.0
90	5.5
91	3.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	7.671064743786438E-4
9	7.671064743786438E-4
10-11	7.671064743786438E-4
12-13	0.0011506597115679656
14-15	7.671064743786438E-4
16-17	0.0015342129487572877
18-19	0.0011506597115679656
20-21	3.835532371893219E-4
22-23	7.671064743786438E-4
24-25	7.671064743786438E-4
26-27	7.671064743786438E-4
28-29	7.671064743786438E-4
30-31	0.0011506597115679656
32-33	7.671064743786438E-4
34-35	7.671064743786438E-4
36-37	7.671064743786438E-4
38-39	0.0011506597115679656
40-41	0.0011506597115679656
42-43	7.671064743786438E-4
44-45	7.671064743786438E-4
46-47	7.671064743786438E-4
48-49	7.671064743786438E-4
50-51	3.835532371893219E-4
52-53	7.671064743786438E-4
54-55	7.671064743786438E-4
56-57	7.671064743786438E-4
58-59	0.0011506597115679656
60-61	0.0011506597115679656
62-63	0.0011506597115679656
64-65	7.671064743786438E-4
66-67	0.0015342129487572877
68-69	0.01841055538508745
70-71	0.0011506597115679656
72-73	7.671064743786438E-4
74-75	7.671064743786438E-4
76	0.0015342129487572877
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	130360.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.99378052414289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.37224913214843	91.54764282677328
2	2.6920179058805402	5.114499162365576
3	0.5817652484889925	1.657922409946104
4	0.18813825189692548	0.714878552355698
5	0.08193109306887304	0.3891472136543825
6	0.04594605517653701	0.26187536888340696
7	0.010986327753674405	0.07305429651791924
8	0.011984906764255608	0.09107932822128102
9	0.004993695473476279	0.04269330106306304
>10	0.009987383348324122	0.10720754021931753
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0023013194231359313	0.0
2	0.0	0.0	0.0	0.0023013194231359313	0.0
3	0.0	0.0	0.0	0.0023013194231359313	0.0
4	0.0	0.0	0.0	0.010739490641301013	0.0
5	0.0	0.0	0.0	0.010739490641301013	0.0
6	0.0	0.0	0.0	0.010739490641301013	0.0
7	0.0	0.0	0.0	0.011506597115679657	0.0
8	0.0	0.0	0.0	0.011506597115679657	0.0
9	0.0	0.0	0.0	0.013040810064436944	0.0
10	0.0	0.0	0.0	0.013807916538815588	0.0
11	0.0	0.0	0.0	0.014575023013194232	0.0
12	0.0	0.0	0.0	0.014575023013194232	0.0
13	0.0	0.0	0.0	0.014575023013194232	0.0
14	0.0	0.0	0.0	0.016109235961951518	0.0
15	0.0	0.0	0.0	0.016109235961951518	0.0
16	0.0	0.0	0.0	0.016876342436330162	0.0
17	0.0	0.0	0.0	0.01841055538508745	0.0
18	0.0	0.0	0.0	0.01841055538508745	0.0
19	0.0	0.0	0.0	0.019944768333844738	0.0
20	0.0	0.0	0.0	0.020711874808223382	0.0
21	0.0	0.0	0.0	0.0245474071801166	0.0
22	0.0	0.0	0.0	0.029917152500767107	0.0
23	0.0	0.0	0.0	0.033752684872660324	0.0
24	0.0	0.0	0.0	0.03528689782141761	0.0
25	0.0	0.0	0.0	0.039889536667689476	0.0
26	0.0	0.0	0.0	0.0437250690395827	0.0
27	0.0	0.0	0.0	0.09205277692543726	0.0
28	0.0	0.0	0.0	0.12887388769561214	0.0
29	0.0	0.0	0.0	0.15188708192697145	0.0
30	0.0	0.0	0.0	0.18027002147898127	0.0
31	0.0	0.0	0.0	0.1963792574409328	0.0
32	0.0	0.0	0.0	0.2278306228904572	7.671064743786437E-4
33	0.0	0.0	0.0	0.2600490948143602	7.671064743786437E-4
34	0.0	0.0	0.0	0.3083768027002148	7.671064743786437E-4
35	0.0	0.0	0.0	0.41807302853636086	7.671064743786437E-4
36	0.0	0.0	0.0	0.5239337220006137	7.671064743786437E-4
37	0.0	0.0	0.0	0.8307763117520712	7.671064743786437E-4
38	0.0	0.0	0.0	1.1422215403498006	7.671064743786437E-4
39	0.0	0.0	0.0	1.580239337220006	7.671064743786437E-4
40	0.0	0.0	0.0	2.1095428045412703	7.671064743786437E-4
41	0.0	0.0	0.0	2.9057993249463023	7.671064743786437E-4
42	7.671064743786437E-4	0.0	0.0	3.751150659711568	7.671064743786437E-4
43	7.671064743786437E-4	0.0	0.0	4.685486345504756	7.671064743786437E-4
44	7.671064743786437E-4	0.0	0.0	5.477907333537895	7.671064743786437E-4
45	7.671064743786437E-4	0.0	0.0	5.983430500153421	7.671064743786437E-4
46	7.671064743786437E-4	0.0	0.0	6.567198527155569	7.671064743786437E-4
47	7.671064743786437E-4	0.0	0.0	7.310524700828475	7.671064743786437E-4
48	7.671064743786437E-4	0.0	0.0	7.814513654495244	7.671064743786437E-4
49	7.671064743786437E-4	0.0	0.0	8.273243326173674	7.671064743786437E-4
50	7.671064743786437E-4	0.0	0.0	8.968241791960724	7.671064743786437E-4
51	7.671064743786437E-4	0.0	0.0	9.83583921448297	7.671064743786437E-4
52	7.671064743786437E-4	0.0	0.0	10.61598649892605	7.671064743786437E-4
53	7.671064743786437E-4	0.0	0.0	11.677661859466093	7.671064743786437E-4
54	7.671064743786437E-4	0.0	0.0	12.549094814360233	7.671064743786437E-4
55	7.671064743786437E-4	0.0	0.0	13.159711567965633	7.671064743786437E-4
56	7.671064743786437E-4	0.0	0.0	13.751917766185947	7.671064743786437E-4
57	7.671064743786437E-4	0.0	0.0	14.231359312672598	7.671064743786437E-4
58	7.671064743786437E-4	0.0	0.0	14.650199447683338	7.671064743786437E-4
59	7.671064743786437E-4	0.0	0.0	15.087450138079165	7.671064743786437E-4
60	7.671064743786437E-4	0.0	0.0	15.40196379257441	7.671064743786437E-4
61	7.671064743786437E-4	0.0	0.0	15.73565510892912	7.671064743786437E-4
62	7.671064743786437E-4	0.0	0.0	15.998772629640994	7.671064743786437E-4
63	7.671064743786437E-4	0.0	0.0	16.3577784596502	7.671064743786437E-4
64	7.671064743786437E-4	0.0	0.0	16.712181650813132	7.671064743786437E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCTT	15	0.0022205184	69.99962	44
GCTCTAA	20	0.006938438	52.499714	13
GTATGCC	20	0.006938438	52.499714	11
TCTAAGG	20	0.006938438	52.499714	3
CGGTCCA	20	0.006938438	52.499714	52
TATACGC	20	0.006938438	52.499714	52
GATCTCG	50	6.145092E-8	48.999733	41
GTAGCTC	30	5.8602943E-4	46.66641	38
CTCGGTG	90	0.0	46.66641	44
TCTCGGT	85	1.8189894E-12	45.29387	43
ATCTCGG	55	1.3007048E-7	44.54521	42
GTGTTCG	50	3.4572422E-6	41.99977	48
CAGCTGA	60	2.5763256E-7	40.833107	55
ACTTGGA	35	0.001251712	39.99978	17
CCAGAGT	35	0.001251712	39.99978	28
TTAGTCT	35	0.001251712	39.99978	38
TAGATCT	70	1.9059371E-8	39.99978	39
GAGTCTA	45	9.1573726E-5	38.888676	34
TCTTACG	45	9.1573726E-5	38.888676	54
ACCAGAG	45	9.1573726E-5	38.888676	27
>>END_MODULE