FastQCFastQC Report
Wed 25 May 2016
SRR1779755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130360
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT94977.28521018717398TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG11620.891377723227984TruSeq Adapter, Index 5 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT7410.568425897514575TruSeq Adapter, Index 5 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC5160.3958269407793802RNA PCR Primer, Index 5 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC4290.3290886775084382RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC4260.32678735808530224TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2640.20251610923596197RNA PCR Primer, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT2300.17643448910708806No Hit
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC1530.1173672905799325TruSeq Adapter, Index 5 (95% over 24bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1530.1173672905799325No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1420.10892911936176743TruSeq Adapter, Index 5 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1320.10125805461798099TruSeq Adapter, Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATC150.002220469870.015
AATCCGC150.002220469870.010
GGGTCTA207.902318E-570.01
GAATCCG150.002220469870.09
CACAGAC150.002220469870.051
TCTTTAT700.060.01
TGGAATT200.006938287552.523
AGAGCTT200.006938287552.534
CTAGGGA305.860135E-446.6666644
ATCTCGT14600.045.78767437
TCTCGTA14600.045.78767438
TCGTATG15450.045.5339840
TATGCCG15600.045.32051543
CGTCTTC15450.045.30744648
CCGTCTT15450.045.30744647
CTCGTAT15250.045.21311639
GCCGTCT15600.045.09615346
GATCTCG14850.045.01683836
CGTATGC15650.044.9520841
CTTGAAA15200.044.90131457
Produced by FastQC (version 0.11.5)