##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779755_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130360
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.41642374961645	34.0	34.0	34.0	33.0	34.0
2	33.425843817121816	34.0	34.0	34.0	34.0	34.0
3	33.45492482356551	34.0	34.0	34.0	34.0	34.0
4	33.50820036821111	34.0	34.0	34.0	34.0	34.0
5	33.499754525928196	34.0	34.0	34.0	34.0	34.0
6	36.91428352255293	38.0	37.0	38.0	35.0	38.0
7	36.94008898435103	38.0	37.0	38.0	35.0	38.0
8	36.7301856397668	38.0	37.0	38.0	34.0	38.0
9	36.77131788892298	38.0	37.0	38.0	34.0	38.0
10-11	36.65820420374348	38.0	37.0	38.0	34.0	38.0
12-13	36.62697146363915	38.0	37.0	38.0	34.0	38.0
14-15	36.67471617060448	38.0	37.0	38.0	34.0	38.0
16-17	36.62386468241792	38.0	37.0	38.0	34.0	38.0
18-19	36.623718932187785	38.0	37.0	38.0	34.0	38.0
20-21	36.671195151887076	38.0	37.0	38.0	34.0	38.0
22-23	36.55555768640687	38.0	37.0	38.0	34.0	38.0
24-25	36.51199370972691	38.0	37.0	38.0	34.0	38.0
26-27	36.36610923596196	38.0	37.0	38.0	34.0	38.0
28-29	36.31678812519178	38.0	37.0	38.0	34.0	38.0
30-31	36.165039122430194	38.0	37.0	38.0	34.0	38.0
32-33	36.13772629640994	38.0	37.0	38.0	33.5	38.0
34-35	36.078793341515805	38.0	37.0	38.0	33.5	38.0
36-37	36.213800245474076	38.0	37.0	38.0	34.0	38.0
38-39	35.76408791040197	38.0	37.0	38.0	31.0	38.0
40-41	36.11327861307149	38.0	37.0	38.0	34.0	38.0
42-43	36.28252915004603	38.0	37.0	38.0	34.0	38.0
44-45	36.28074179196072	38.0	37.0	38.0	34.0	38.0
46-47	36.323300859159254	38.0	37.0	38.0	34.0	38.0
48-49	36.32157870512427	38.0	37.0	38.0	34.0	38.0
50-51	36.26041730592206	38.0	37.0	38.0	34.0	38.0
52-53	36.26027922675667	38.0	37.0	38.0	34.0	38.0
54-55	36.251123810984964	38.0	37.0	38.0	34.0	38.0
56-57	36.26726373120589	38.0	37.0	38.0	34.0	38.0
58-59	36.214337220006136	38.0	37.0	38.0	34.0	38.0
60-61	36.238447376495856	38.0	37.0	38.0	34.0	38.0
62-63	36.21607855170298	38.0	37.0	38.0	34.0	38.0
64-65	36.22220006136851	38.0	37.0	38.0	34.0	38.0
66-67	36.16747084995397	38.0	37.0	38.0	34.0	38.0
68-69	35.991684565817735	38.0	37.0	38.0	32.0	38.0
70-71	35.28172368824793	38.0	36.5	38.0	28.0	38.0
72-73	34.19220619822032	38.0	36.0	38.0	22.5	38.0
74-75	34.07038969008899	38.0	36.0	38.0	21.5	38.0
76	33.38634550475606	37.0	34.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	5.0
22	19.0
23	38.0
24	60.0
25	110.0
26	256.0
27	423.0
28	726.0
29	1220.0
30	2051.0
31	3057.0
32	4677.0
33	7077.0
34	10919.0
35	17596.0
36	23442.0
37	58681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.75943540963486	16.53344584228291	10.825406566431422	31.881712181650812
2	21.800398895366676	18.533292420988033	35.505523166615525	24.160785517029765
3	19.289659404725377	20.393525621356247	34.34795949677815	25.96885547714023
4	14.811291807302855	23.079165388155875	32.79917152500767	29.310371279533598
5	14.429272783062288	31.19285056765879	38.529456888616146	15.848419760662779
6	28.150506290273093	39.349493709726914	17.20696532678736	15.293034673212642
7	27.46087756980669	27.529917152500765	18.84166922368825	26.167536054004298
8	23.80638232586683	38.1750536974532	17.674900276158333	20.343663700521635
9	22.651887081926972	17.42252224608776	18.291653881558762	41.63393679042651
10-11	24.218318502608163	26.32747775391224	26.825713409021173	22.628490334458423
12-13	24.24094814360233	19.687020558453515	27.44860386621663	28.62342743172752
14-15	22.59665541577171	20.15111997545259	21.257287511506597	35.9949370972691
16-17	27.42060447990181	27.33162012887389	21.093510279226756	24.154265111997546
18-19	27.189321877876647	23.68249463025468	21.96724455354403	27.16093893832464
20-21	22.112611230438787	23.069193003988953	21.981052470082847	32.83714329548941
22-23	22.848266339367903	27.625805461798098	21.841439091745933	27.684489107088062
24-25	27.611613992022093	27.463562442467016	21.632402577477755	23.29242098803314
26-27	26.848343050015345	28.481129180730285	22.280223995090516	22.390303774163854
28-29	22.080392758514883	28.51373120589138	22.85632095734888	26.549555078244865
30-31	22.001380791653883	28.489567351948452	22.429809757594356	27.079242098803313
32-33	21.888616139920224	33.27017490027615	22.73243326173673	22.10877569806689
34-35	27.34657870512427	24.041500460263883	27.31742865909788	21.294492175513962
36-37	27.293264805154955	26.483583921448293	24.227907333537896	21.995243939858852
38-39	22.142144829702364	23.335762503835532	27.909634857318196	26.61245780914391
40-41	21.522706351641606	21.502761583307763	29.224838907640383	27.74969315741025
42-43	26.072414851181342	21.207809143909177	29.13547100337527	23.584305001534215
44-45	21.24463025467935	25.54924823565511	30.321034059527463	22.885087450138077
46-47	25.550782448603865	20.717628106781223	26.07548327707886	27.656106167536056
48-49	24.731896287204663	20.53122123350721	25.714176127646514	29.02270635164161
50-51	20.312979441546485	21.240794722307456	30.518180423442775	27.928045412703284
52-53	18.80638232586683	21.271095428045413	35.35670451058607	24.56581773550169
54-55	18.570497084995395	21.366216630868365	31.315587603559376	28.747698680576868
56-57	22.88355323718932	21.59634857318196	26.427585148818654	29.092513040810065
58-59	18.849723841669224	21.598266339367907	34.141991408407485	25.410018410555384
60-61	22.83982816814974	26.75897514575023	25.674670144216016	24.726526541884013
62-63	18.41170604479902	33.24831236575637	23.028536360846886	25.31144522859773
64-65	18.78988953666769	34.10785517029763	21.785440319116294	25.316814973918376
66-67	18.46041730592206	33.959803620742555	21.01181343970543	26.567965633629946
68-69	18.300859159251303	34.115909788278614	21.09120895980362	26.492022092666463
70-71	18.921064743786438	33.19538201902424	21.17789199140841	26.705661245780917
72-73	19.398588524087142	31.08046946916232	20.97959496778153	28.54134703896901
74-75	19.66055538508745	30.755216324025774	21.16331696839521	28.42091132249156
76	19.46916231972998	31.00184105553851	21.07011353175821	28.458883092973302
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	1.5
15	5.5
16	9.0
17	10.0
18	11.5
19	23.5
20	36.5
21	39.0
22	55.0
23	95.5
24	171.5
25	223.0
26	290.5
27	374.5
28	457.0
29	523.0
30	711.0
31	963.0
32	1281.5
33	1536.0
34	1756.5
35	2245.5
36	2789.5
37	3065.0
38	3643.0
39	6464.5
40	9487.5
41	9791.5
42	9316.0
43	8791.5
44	7945.5
45	7370.5
46	7117.0
47	6976.0
48	6463.0
49	5957.0
50	5823.0
51	5474.0
52	4513.5
53	3610.0
54	3318.0
55	3046.5
56	2546.0
57	2153.0
58	1989.0
59	1868.0
60	1710.0
61	1645.5
62	1618.0
63	1619.0
64	1609.0
65	1611.5
66	1625.0
67	1625.0
68	1653.5
69	1604.5
70	1526.5
71	1526.0
72	1444.5
73	1303.0
74	1208.5
75	1174.0
76	1092.0
77	901.5
78	703.0
79	613.0
80	539.0
81	395.5
82	304.0
83	282.0
84	216.0
85	122.5
86	71.5
87	48.0
88	38.5
89	22.5
90	10.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	130360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.260049094814356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.00237284113828	27.15710340595275
2	21.108116811579464	19.10708806382326
3	8.64900594905171	11.743633016262658
4	3.825358892222166	6.925437250690396
5	1.8423416552261824	4.1692236882479285
6	1.1948950017796307	3.244860386621663
7	0.8237148522906391	2.6096962258361462
8	0.571176759715937	2.0681190549248236
9	0.43389095100083047	1.7674133169683952
>10	1.5186183285029067	10.154955507824486
>50	0.005084659582040982	0.17106474378643757
>100	0.018643751800816937	1.7405645903651425
>500	0.003389773054693988	0.9642528382939553
>1k	0.001694886527346994	0.891377723227984
>5k	0.001694886527346994	7.28521018717398
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	9497	7.28521018717398	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	1162	0.891377723227984	TruSeq Adapter, Index 5 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	741	0.568425897514575	TruSeq Adapter, Index 5 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	516	0.3958269407793802	RNA PCR Primer, Index 5 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	429	0.3290886775084382	RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	426	0.32678735808530224	TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	264	0.20251610923596197	RNA PCR Primer, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	230	0.17643448910708806	No Hit
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	153	0.1173672905799325	TruSeq Adapter, Index 5 (95% over 24bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	153	0.1173672905799325	No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	142	0.10892911936176743	TruSeq Adapter, Index 5 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	132	0.10125805461798099	TruSeq Adapter, Index 5 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0023013194231359313	0.0
2	0.0	0.0	0.0	0.003068425897514575	0.0
3	0.0	0.0	0.0	0.003068425897514575	0.0
4	0.0	0.0	0.0	0.013807916538815588	0.0
5	0.0	0.0	0.0	0.014575023013194232	0.0
6	0.0	0.0	0.0	0.014575023013194232	0.0
7	0.0	0.0	0.0	0.015342129487572876	0.0
8	0.0	0.0	0.0	0.015342129487572876	0.0
9	0.0	0.0	0.0	0.017643448910708806	0.0
10	0.0	0.0	0.0	0.01841055538508745	0.0
11	0.0	0.0	0.0	0.01841055538508745	0.0
12	0.0	0.0	0.0	0.019177661859466094	0.0
13	0.0	0.0	0.0	0.019177661859466094	0.0
14	0.0	0.0	0.0	0.020711874808223382	0.0
15	0.0	0.0	0.0	0.020711874808223382	0.0
16	0.0	0.0	0.0	0.021478981282602026	0.0
17	0.0	0.0	0.0	0.0245474071801166	0.0
18	0.0	0.0	0.0	0.025314513654495243	0.0
19	0.0	0.0	0.0	0.027615833077631175	0.0
20	0.0	0.0	0.0	0.03068425897514575	0.0
21	0.0	0.0	0.0	0.033752684872660324	0.0
22	0.0	0.0	0.0	0.03451979134703897	0.0
23	0.0	0.0	0.0	0.039889536667689476	0.0
24	0.0	0.0	0.0	0.041423749616446764	0.0
25	0.0	0.0	0.0	0.04986192083461184	0.0
26	0.0	0.0	0.0	0.054464559680883706	0.0
27	0.0	0.0	0.0	0.12964099416999078	0.0
28	0.0	0.0	0.0	0.1825713409021172	0.0
29	0.0	0.0	0.0	0.23396747468548634	0.0
30	0.0	0.0	0.0	0.2807609696225836	0.0
31	0.0	0.0	0.0	0.3329242098803314	0.0
32	0.0	0.0	0.0	0.3927585148818656	0.0
33	0.0	0.0	0.0	0.46486652347345814	0.0
34	0.0	0.0	0.0	0.5853022399509051	0.0
35	0.0	0.0	0.0	0.7747775391224302	0.0
36	0.0	0.0	0.0	1.0961951518870818	0.0
37	0.0	0.0	0.0	1.5618287818349186	0.0
38	0.0	0.0	0.0	2.103405952746241	0.0
39	0.0	0.0	0.0	2.827554464559681	0.0
40	0.0	0.0	0.0	3.6123043878490333	0.0
41	0.0	0.0	0.0	4.687787664927892	0.0
42	0.0	0.0	0.0	5.930500153421295	0.0
43	0.0	0.0	0.0	7.241485118134397	0.0
44	0.0	0.0	0.0	8.335378950598344	0.0
45	0.0	0.0	0.0	9.35486345504756	0.0
46	0.0	0.0	0.0	10.40119668610003	0.0
47	0.0	0.0	0.0	11.438324639459957	0.0
48	0.0	0.0	0.0	12.342743172752378	0.0
49	0.0	0.0	0.0	13.260969622583614	0.0
50	0.0	0.003068425897514575	0.0	14.12856704510586	0.0
51	0.0	0.003068425897514575	0.0	15.141914697760049	0.0
52	0.0	0.003068425897514575	0.0	16.26035593740411	0.0
53	0.0	0.003068425897514575	0.0	17.420988033139	0.0
54	0.0	0.003068425897514575	0.0	18.397514575023013	0.0
55	0.0	0.003068425897514575	0.0	19.347192390303775	0.0
56	0.0	0.003068425897514575	0.0	20.185639766799632	0.0
57	0.0	0.003068425897514575	0.0	20.93817121816508	0.0
58	0.0	0.003068425897514575	0.0	21.6738263270942	0.0
59	0.0	0.003068425897514575	0.0	22.32433261736729	0.0
60	0.0	0.003068425897514575	0.0	23.012427124884933	0.0
61	0.0	0.003068425897514575	0.0	23.725069039582696	0.0
62	0.0	0.003068425897514575	0.0	24.481436023320036	0.0
63	0.0	0.003068425897514575	0.0	25.25467934949371	0.0
64	0.0	0.003068425897514575	0.0	26.05860693464253	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATC	15	0.0022204698	70.0	15
AATCCGC	15	0.0022204698	70.0	10
GGGTCTA	20	7.902318E-5	70.0	1
GAATCCG	15	0.0022204698	70.0	9
CACAGAC	15	0.0022204698	70.0	51
TCTTTAT	70	0.0	60.0	1
TGGAATT	20	0.0069382875	52.5	23
AGAGCTT	20	0.0069382875	52.5	34
CTAGGGA	30	5.860135E-4	46.666664	4
ATCTCGT	1460	0.0	45.787674	37
TCTCGTA	1460	0.0	45.787674	38
TCGTATG	1545	0.0	45.53398	40
TATGCCG	1560	0.0	45.320515	43
CGTCTTC	1545	0.0	45.307446	48
CCGTCTT	1545	0.0	45.307446	47
CTCGTAT	1525	0.0	45.213116	39
GCCGTCT	1560	0.0	45.096153	46
GATCTCG	1485	0.0	45.016838	36
CGTATGC	1565	0.0	44.95208	41
CTTGAAA	1520	0.0	44.901314	57
>>END_MODULE