FastQCFastQC Report
Wed 25 May 2016
SRR1779754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124069
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT150.002219351570.0068654
AAACTCG207.896968E-570.0068527
TCGATAT150.002222904769.9786439
CGTGTAT207.912764E-569.9786435
TTACGGT150.002222904769.9786443
GACTAAA301.0189251E-769.9786423
ATGTATA401.05592335E-861.25599728
GAAACTC352.9522016E-760.0058826
GATTTCG352.9604598E-759.9816941
TACGCTT308.331432E-658.33904614
AGATTTC308.351382E-658.31553340
TCTAGAC252.3720646E-456.05068664
ACGTATC252.3815462E-456.0054855
GTAAAGT252.3862983E-455.9829135
ATTTCGG252.3862983E-455.9829142
CGGTGGT1750.053.9835246
TTACGCT850.053.53465313
TCTCGGT2050.052.91067543
ACAAAAG200.006934806752.50514269
CGTATAG200.00694587852.4839835
Produced by FastQC (version 0.11.5)