##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779754_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124069
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.484883411649967	32.0	23.0	34.0	12.0	34.0
2	26.681765791615955	32.0	21.0	34.0	12.0	34.0
3	25.827660414769202	31.0	21.0	34.0	11.0	34.0
4	27.356205014951357	32.0	23.0	34.0	12.0	34.0
5	27.125341543818358	32.0	23.0	34.0	11.0	34.0
6	29.06860698482296	34.0	21.0	38.0	11.0	38.0
7	30.06036157299567	35.0	25.0	38.0	12.0	38.0
8	29.319217532179675	34.0	21.0	38.0	11.0	38.0
9	30.706768008124513	36.0	26.0	38.0	12.0	38.0
10-11	30.20727579008455	36.0	24.5	38.0	11.0	38.0
12-13	29.668527190514954	35.5	23.5	38.0	11.0	38.0
14-15	29.284007286268125	34.0	22.0	38.0	11.0	38.0
16-17	29.094028322949328	34.5	23.5	38.0	11.0	38.0
18-19	29.636073475243613	34.0	23.0	38.0	11.0	38.0
20-21	28.561828498658002	32.5	22.0	37.5	11.0	38.0
22-23	28.64254165021077	33.5	23.0	37.5	11.0	38.0
24-25	29.453183309287574	35.0	22.5	38.0	11.0	38.0
26-27	28.22143726474784	33.0	21.0	38.0	11.0	38.0
28-29	29.898649138785675	36.0	23.0	38.0	11.0	38.0
30-31	30.356454875915823	37.0	24.0	38.0	11.0	38.0
32-33	30.467880776019797	37.0	25.0	38.0	11.0	38.0
34-35	30.535536677171574	37.0	25.0	38.0	11.0	38.0
36-37	28.867984750421137	34.0	22.0	38.0	11.0	38.0
38-39	27.60665033167028	31.0	21.0	37.0	11.0	38.0
40-41	28.274444059354067	34.0	21.0	37.0	11.0	38.0
42-43	29.136226615834737	35.0	22.5	37.5	11.0	38.0
44-45	30.566112405193884	37.0	26.0	38.0	11.0	38.0
46-47	29.412008640353353	35.5	23.5	38.0	11.0	38.0
48-49	28.75426174145032	34.5	22.0	38.0	11.0	38.0
50-51	27.035705937824922	31.0	16.0	37.0	11.0	38.0
52-53	29.67672021214002	35.0	23.0	37.5	11.0	38.0
54-55	28.930171920463614	35.0	22.0	38.0	11.0	38.0
56-57	29.349233088039718	35.5	22.5	38.0	11.0	38.0
58-59	30.194754531752494	36.5	23.5	38.0	11.0	38.0
60-61	30.683498698304973	37.0	25.0	38.0	11.0	38.0
62-63	30.611760391395112	37.0	24.5	38.0	11.0	38.0
64-65	30.387155534420366	36.5	24.0	38.0	11.0	38.0
66-67	28.711543576558206	34.0	21.0	38.0	11.0	38.0
68-69	28.69594741635703	34.0	21.0	38.0	11.0	38.0
70-71	28.38651476194698	34.0	21.0	38.0	11.0	38.0
72-73	27.366159153374333	31.5	20.5	37.0	11.0	38.0
74-75	28.85329937373558	35.0	22.0	37.5	11.0	38.0
76	29.083300421539626	34.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	2.0
5	1.0
6	0.0
7	0.0
8	2.0
9	0.0
10	0.0
11	15.0
12	83.0
13	405.0
14	1039.0
15	1900.0
16	3049.0
17	4576.0
18	5941.0
19	6203.0
20	5284.0
21	4080.0
22	3019.0
23	2470.0
24	2168.0
25	1978.0
26	2208.0
27	2424.0
28	2742.0
29	3207.0
30	3925.0
31	4835.0
32	6099.0
33	7949.0
34	10632.0
35	13774.0
36	15567.0
37	8490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.897766565378944	16.909139269277578	14.132458551290009	33.06063561405347
2	20.20246798152641	15.002135908244608	41.495458172468545	23.29993793776044
3	18.908832988095334	16.221618615447856	39.522362556319464	25.347185840137342
4	15.018255970468047	19.800272429051578	29.383649420886766	35.79782217959362
5	16.120868226551355	25.797741579282494	39.26605356696677	18.81533662719938
6	26.676284970459985	33.36933480563235	15.790406950970832	24.163973272936833
7	25.82756369439586	23.702133490235273	19.15949995566983	31.310802859699038
8	22.02993640327898	32.959867164263315	16.671368578867188	28.338827853590516
9	21.777469693061647	15.41301263863812	18.219628578798037	44.58988908950219
10-11	24.722415898566435	22.455798132377893	26.122527939771807	26.69925802928386
12-13	23.416826196473554	17.421057934508816	29.189722921914356	29.972392947103277
14-15	25.03103237038948	21.16246453443384	21.978575573897345	31.827927521279342
16-17	27.59713042076415	19.38779622763179	23.42213445107206	29.592938900532
18-19	22.632544080604532	19.26125944584383	27.015012594458437	31.091183879093197
20-21	27.019119164302296	19.368229301005933	24.572398117100853	29.04025341759092
22-23	28.146121077046466	18.19258996546068	24.26860871422641	29.392680243266444
24-25	23.302301213073793	23.031878450812073	21.05227098698263	32.6135493491315
26-27	25.969257802424554	21.970515217952023	24.193158369873615	27.867068609749808
28-29	24.365856607423527	22.34554467416274	23.68153790351832	29.60706081489542
30-31	23.82900347409742	21.936386132628304	24.48271414868492	29.751896244589354
32-33	24.100867294299714	21.464727882383286	24.712245292752126	29.722159530564866
34-35	24.27627787383074	21.531715613375408	24.438694700697628	29.75331181209622
36-37	26.806570961059478	17.936740796866058	29.12921869080533	26.127469551269133
38-39	25.991246241768163	16.67445853249611	33.58630028776167	23.747994937974063
40-41	24.683322384462546	21.923803919830085	24.1053832171947	29.287490478512673
42-43	20.925808752705247	22.591071579702493	26.125523417254737	30.357596250337526
44-45	20.742284162078946	19.56022343486777	29.28391220589539	30.4135801971579
46-47	25.229622166246852	18.85662468513854	27.84080604534005	28.072947103274558
48-49	25.026800899542973	19.30793226022263	28.933283896085054	26.731982944149348
50-51	29.53793450881612	17.797481108312343	28.293400503778336	24.3711838790932
52-53	19.76124648358469	21.383835371309274	28.78946647213871	30.06545167296733
54-55	24.173786877317426	19.921408995647266	26.62219893599871	29.28260519103659
56-57	24.31930294041785	20.35199574413206	25.725432035078672	29.603269280371418
58-59	20.700536828360015	22.12240653866615	27.239202979155586	29.93785365381825
60-61	20.269382523990117	20.50273453085768	27.304199933097696	31.923683012054504
62-63	20.253664513164843	22.033023943769827	26.90802545511702	30.805286087948314
64-65	20.689168765743073	23.06498740554156	26.18478589420655	30.061057934508817
66-67	19.01957939367559	26.933555808123554	21.15162946662475	32.8952353315761
68-69	19.262242216095068	27.136442058078973	21.51800254760638	32.08331317821958
70-71	19.071512630781385	28.10892940626461	20.737615063436024	32.08194289951798
72-73	18.032919024358787	29.179764956231562	20.490964195321695	32.29635182408796
74-75	19.62567103545002	25.826199803324144	25.04151150231336	29.506617658912482
76	20.843610452838096	24.15324595766633	26.50045944769551	28.502684141800067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	1.5
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	1.0
14	1.5
15	0.5
16	1.0
17	2.0
18	4.5
19	10.0
20	18.0
21	23.0
22	36.0
23	52.0
24	84.5
25	114.0
26	151.0
27	229.5
28	335.0
29	399.0
30	490.0
31	706.0
32	956.0
33	1081.0
34	1313.0
35	1741.0
36	2215.5
37	2494.0
38	2811.0
39	3498.5
40	4204.0
41	4808.5
42	5078.0
43	5420.0
44	5921.0
45	6181.0
46	6282.0
47	6258.5
48	6020.0
49	5614.0
50	5423.0
51	5196.5
52	4735.0
53	4317.0
54	4134.0
55	3974.0
56	3675.5
57	3515.0
58	3493.0
59	3359.5
60	3180.0
61	3154.0
62	3174.0
63	3033.0
64	2852.5
65	2806.5
66	2729.0
67	2658.0
68	2568.0
69	2463.0
70	2373.5
71	2299.0
72	2169.5
73	1976.0
74	1775.0
75	1638.0
76	1455.0
77	1164.5
78	901.5
79	746.0
80	628.0
81	411.5
82	257.5
83	202.0
84	155.5
85	82.0
86	38.0
87	21.0
88	16.5
89	9.5
90	5.0
91	2.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.004836018667032055
9	0.004030015555860045
10-11	0.0060450233337900686
12-13	0.00523902022261806
14-15	0.004030015555860045
16-17	0.007254028000548082
18-19	0.00523902022261806
20-21	0.004030015555860045
22-23	0.0060450233337900686
24-25	0.003224012444688036
26-27	0.004030015555860045
28-29	0.003224012444688036
30-31	0.006448024889376072
32-33	0.004030015555860045
34-35	0.00443301711144605
36-37	0.006448024889376072
38-39	0.006448024889376072
40-41	0.0060450233337900686
42-43	0.003627014000274041
44-45	0.004836018667032055
46-47	0.00523902022261806
48-49	0.004836018667032055
50-51	0.00523902022261806
52-53	0.006448024889376072
54-55	0.007254028000548082
56-57	0.004030015555860045
58-59	0.005642021778204063
60-61	0.0060450233337900686
62-63	0.0060450233337900686
64-65	0.00523902022261806
66-67	0.00806003111172009
68-69	0.024986096446332283
70-71	0.005642021778204063
72-73	0.005642021778204063
74-75	0.005642021778204063
76	0.005642021778204063
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	124069.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.42280765478876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.62110534551779	92.19857150778425
2	2.5317788725357753	4.831788967568784
3	0.5341945926068664	1.5292304358165978
4	0.17801230241122867	0.6794573477269105
5	0.06995697880764401	0.3337745666435974
6	0.035973709731750236	0.2059627430617203
7	0.0139895017393613	0.09344422735629841
8	0.005995480840811008	0.0457684492056544
9	0.004996231225210582	0.04290789700819071
>10	0.003996984583572703	0.03909385782800806
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004030015555860046	0.0
2	0.0	0.0	0.0	0.004030015555860046	0.0
3	0.0	0.0	0.0	0.004030015555860046	0.0
4	0.0	0.0	0.0	0.0088660342228921	0.0
5	0.0	0.0	0.0	0.010478040445236118	0.0
6	0.0	0.0	0.0	0.010478040445236118	0.0
7	0.0	0.0	0.0	0.010478040445236118	0.0
8	0.0	0.0	0.0	0.011284043556408128	0.0
9	0.0	0.0	0.0	0.011284043556408128	0.0
10	0.0	0.0	0.0	0.013702052889924154	0.0
11	0.0	0.0	0.0	0.016926065334612193	0.0
12	0.0	0.0	0.0	0.016926065334612193	0.0
13	0.0	0.0	0.0	0.0177320684457842	0.0
14	0.0	0.0	0.0	0.01934407466812822	0.0
15	0.0	0.0	0.0	0.022568087112816257	0.0
16	0.0	0.0	0.0	0.02579209955750429	0.0
17	0.0	0.0	0.0	0.02579209955750429	0.0
18	0.0	0.0	0.0	0.0265981026686763	0.0
19	0.0	0.0	0.0	0.02901611200219233	0.0
20	0.0	0.0	0.0	0.03224012444688037	0.0
21	0.0	0.0	0.0	0.0354641368915684	0.0
22	0.0	0.0	0.0	0.05239020222618059	0.0
23	0.0	0.0	0.0	0.056420217782040635	0.0
24	0.0	0.0	0.0	0.06851026444962077	0.0
25	0.0	0.0	0.0	0.07012227067196479	0.0
26	0.0	0.0	0.0	0.07254028000548082	0.0
27	0.0	0.0	0.0	0.11203443245290927	0.0
28	0.0	0.0	0.0	0.1587826129008858	0.0
29	0.0	0.0	0.0	0.19021673423659416	0.0
30	0.0	0.0	0.0	0.2458309489074628	0.0
31	0.0	0.0	0.0	0.2788770764655152	0.0
32	0.0	0.0	0.0	0.31917723202411563	0.0
33	0.0	0.0	0.0	0.37801545913967227	0.0
34	0.0	0.0	0.0	0.46989981381328133	8.060031111720091E-4
35	0.0	0.0	0.0	0.6472204982711234	8.060031111720091E-4
36	0.0	0.0	0.0	0.8076151173943531	8.060031111720091E-4
37	0.0	0.0	0.0	1.2791269374299785	8.060031111720091E-4
38	0.0	0.0	0.0	1.669232443237231	8.060031111720091E-4
39	0.0	0.0	0.0	2.109310141937148	8.060031111720091E-4
40	0.0	0.0	0.0	2.5872699868621494	8.060031111720091E-4
41	0.0	0.0	0.0	3.0652298317871507	8.060031111720091E-4
42	0.0	0.0	0.0	3.5512497078238723	8.060031111720091E-4
43	0.0	0.0	0.0	4.008253471858401	8.060031111720091E-4
44	0.0	0.0	0.0	4.53699151278724	8.060031111720091E-4
45	0.0	0.0	0.0	4.923873006149804	8.060031111720091E-4
46	0.0	0.0	0.0	5.400220844852461	8.060031111720091E-4
47	0.0	0.0	0.0	5.908002804890827	8.060031111720091E-4
48	0.0	0.0	0.0	6.207835962246814	8.060031111720091E-4
49	0.0	0.0	0.0	6.521371172492726	8.060031111720091E-4
50	0.0	0.0	0.0	6.934850768523966	8.060031111720091E-4
51	0.0	0.0	0.0	7.444244734784676	8.060031111720091E-4
52	0.0	0.0	0.0	7.8472462903706806	8.060031111720091E-4
53	0.0	0.0	0.0	8.30828006996107	8.060031111720091E-4
54	0.0	0.0	0.0	8.827346073555844	8.060031111720091E-4
55	0.0	0.0	0.0	9.148941314913476	8.060031111720091E-4
56	0.0	0.0	0.0	9.510836711829707	8.060031111720091E-4
57	0.0	0.0	0.0	9.827595934520307	8.060031111720091E-4
58	0.0	0.0	0.0	10.08148691453949	8.060031111720091E-4
59	0.0	0.0	0.0	10.420008221231734	8.060031111720091E-4
60	0.0	0.0	0.0	10.633599045692316	8.060031111720091E-4
61	0.0	0.0	0.0	10.8471898701529	8.060031111720091E-4
62	0.0	0.0	0.0	10.98421039905214	8.060031111720091E-4
63	0.0	0.0	0.0	11.198607226623896	8.060031111720091E-4
64	0.0	0.0	0.0	11.381569932859941	8.060031111720091E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	15	0.0022193515	70.00686	54
AAACTCG	20	7.896968E-5	70.00685	27
TCGATAT	15	0.0022229047	69.97864	39
CGTGTAT	20	7.912764E-5	69.97864	35
TTACGGT	15	0.0022229047	69.97864	43
GACTAAA	30	1.0189251E-7	69.97864	23
ATGTATA	40	1.05592335E-8	61.255997	28
GAAACTC	35	2.9522016E-7	60.00588	26
GATTTCG	35	2.9604598E-7	59.98169	41
TACGCTT	30	8.331432E-6	58.339046	14
AGATTTC	30	8.351382E-6	58.315533	40
TCTAGAC	25	2.3720646E-4	56.050686	64
ACGTATC	25	2.3815462E-4	56.00548	55
GTAAAGT	25	2.3862983E-4	55.98291	35
ATTTCGG	25	2.3862983E-4	55.98291	42
CGGTGGT	175	0.0	53.98352	46
TTACGCT	85	0.0	53.534653	13
TCTCGGT	205	0.0	52.910675	43
ACAAAAG	20	0.0069348067	52.505142	69
CGTATAG	20	0.006945878	52.48398	35
>>END_MODULE