FastQCFastQC Report
Wed 25 May 2016
SRR1779754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124069
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2526120.360444591316124No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG29322.3632011219563305No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC16961.3669812765477276No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT10610.8551693009535016RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC8030.6472204982711233No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC6580.5303500471511821No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC4220.3401333129145878RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT3500.2821010889102032No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2620.21117281512706637No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2550.20553079334886235No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2060.16603664090143389No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2060.16603664090143389No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT1930.15555860045619777RNA PCR Primer, Index 48 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC1350.10881042000822125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCT150.002220215770.0000134
AAGACAA150.002220215770.0000170
TTACATT150.002220215770.0000126
GCCAATC207.9008125E-570.033
CTAGCCT252.382703E-455.9999964
CGAGACA452.6773705E-854.44444323
ACAAATA200.006937552.570
CTCAACG200.006937552.546
TTTCGCC200.006937552.529
TGGCGAT200.006937552.519
ACTTCGA200.006937552.564
CCGCTCT200.006937552.54
TTGACTA200.006937552.533
GTTTTGG200.006937552.523
AGCCTTA200.006937552.56
TTTGACT200.006937552.532
AGAGCGG200.006937552.510
AATCTCG35500.052.15493436
GTCAATC35250.051.92907333
CTTGAAA37100.051.69811657
Produced by FastQC (version 0.11.5)