FastQCFastQC Report
Wed 25 May 2016
SRR1779753_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779753_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234271
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG200.006946923752.49663524
ATGGAAT407.4618583E-752.49663522
CGTTAGT200.006946923752.49663537
GCCGTAT1150.051.7358254
GAGTGAC305.8716215E-446.663687
TAGATCT1900.044.20769539
TTCGTGT404.58529E-543.74719633
GTAGATC1450.043.44549638
ACGCTTC1150.042.6059715
CTCTCGG757.530616E-1041.9973142
ATCTCGT1000.041.99730742
GACGCTT1000.041.99730714
TGGTCGC1700.041.17383649
GTAATGG350.001254118339.99743758
GCCGAAA801.4151738E-939.3724870
CTTCCGA1250.039.1974918
CGCCGTA1650.038.17937553
AGATCTC2250.037.3309440
TCGGTGG2400.036.45645
CGACACT501.7005613E-435.02018468
Produced by FastQC (version 0.11.5)