##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779753_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234271
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.36637910795617	34.0	27.0	34.0	12.0	34.0
2	27.83891732224646	34.0	23.0	34.0	12.0	34.0
3	26.78738298807791	32.0	21.0	34.0	11.0	34.0
4	29.095329767662236	34.0	23.0	34.0	12.0	34.0
5	28.66665101527718	34.0	23.0	34.0	11.0	34.0
6	31.007901105984097	36.0	27.0	38.0	11.0	38.0
7	31.961348182233397	37.0	28.0	38.0	12.0	38.0
8	31.197288610199298	37.0	27.0	38.0	11.0	38.0
9	32.65828463616922	37.0	31.0	38.0	12.0	38.0
10-11	32.340780549022284	37.0	31.0	38.0	11.0	38.0
12-13	31.366773949827337	36.0	28.5	38.0	11.0	38.0
14-15	31.380766292029314	36.5	27.0	38.0	11.0	38.0
16-17	31.051506588523544	36.0	27.0	38.0	11.0	38.0
18-19	31.617857097122563	37.0	29.0	38.0	11.5	38.0
20-21	30.5117321392746	35.5	26.5	38.0	11.0	38.0
22-23	30.511211374860736	35.5	26.0	38.0	11.0	38.0
24-25	31.65809041665422	36.5	29.0	38.0	11.0	38.0
26-27	30.245124236461194	35.5	24.5	38.0	11.0	38.0
28-29	32.34453688249933	37.0	31.0	38.0	11.0	38.0
30-31	32.8220799842917	37.0	33.0	38.0	11.0	38.0
32-33	32.91113496762297	38.0	34.0	38.0	11.0	38.0
34-35	32.95682777637864	38.0	34.0	38.0	11.0	38.0
36-37	30.860356168710595	36.5	27.0	38.0	11.0	38.0
38-39	29.118655317986434	34.0	23.0	37.5	11.0	38.0
40-41	30.293770889269265	35.0	25.0	37.5	11.0	38.0
42-43	31.362420017842584	36.5	28.0	38.0	11.0	38.0
44-45	33.00829594785526	37.5	33.5	38.0	11.0	38.0
46-47	31.550838558763143	37.0	28.5	38.0	11.0	38.0
48-49	30.9674372841709	36.0	27.0	38.0	11.0	38.0
50-51	28.77661767781757	34.0	21.5	37.5	11.0	38.0
52-53	32.146447917155776	36.5	29.5	38.0	11.0	38.0
54-55	31.18588515010394	36.5	27.0	38.0	11.0	38.0
56-57	31.615270349296324	36.5	28.5	38.0	11.0	38.0
58-59	32.73145203631691	37.0	32.5	38.0	11.0	38.0
60-61	33.223905220876674	38.0	34.0	38.0	11.0	38.0
62-63	33.101009087765874	38.0	34.0	38.0	11.0	38.0
64-65	32.787852529762546	37.5	32.5	38.0	11.0	38.0
66-67	30.702278557738687	36.5	25.0	38.0	11.0	38.0
68-69	30.8140956413726	36.5	26.0	38.0	11.0	38.0
70-71	30.419475735366305	35.5	25.0	38.0	11.0	38.0
72-73	29.18569946771047	34.0	22.5	37.5	11.0	38.0
74-75	31.28400655650934	36.5	28.0	38.0	11.0	38.0
76	31.696680340289664	37.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	2.0
5	0.0
6	1.0
7	5.0
8	1.0
9	1.0
10	5.0
11	13.0
12	66.0
13	365.0
14	990.0
15	1709.0
16	2835.0
17	4422.0
18	5927.0
19	6203.0
20	5587.0
21	4727.0
22	3890.0
23	3566.0
24	3538.0
25	3882.0
26	4254.0
27	5045.0
28	5968.0
29	6993.0
30	8602.0
31	10997.0
32	13868.0
33	18495.0
34	24496.0
35	31964.0
36	35893.0
37	19955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80616038690235	18.556714232662173	10.819094126033525	29.818031254401955
2	21.330425020595804	16.44420350790324	37.52619829172198	24.699173179778974
3	21.425278524779102	16.870704742391258	34.450420455030525	27.25359627779912
4	15.41702416473511	17.80788587381065	31.77698950342985	34.99810045802439
5	15.9729543989653	28.58228291167067	37.47540241856653	17.969360270797495
6	30.01609247410051	31.443926051453232	15.577258815645129	22.962722658801134
7	29.070606263686074	24.438364116770746	19.92009254239748	26.5709370771457
8	24.99957312387945	30.491761290873388	17.223597711943995	27.285067873303166
9	23.941978502335033	15.005250621110058	18.827532037326367	42.22523883922854
10-11	26.397314015188705	22.20611902515656	25.71621281264274	25.68035414701199
12-13	25.649826044267996	18.643572175620584	27.575291882777314	28.1313098973341
14-15	24.68005361609849	21.12371829350545	22.834012072159755	31.362216018236307
16-17	25.93918359331643	21.692579595809534	25.224123357495966	27.144113453378072
18-19	23.848828497697976	21.47295309935304	26.395888162458565	28.282330240490417
20-21	25.131052087868934	21.217845281697954	26.63601669953641	27.015085930896703
22-23	25.966275346851653	20.669797225186766	26.637566702241195	26.726360725720383
24-25	24.23060751004762	23.290624286314962	22.939944804799804	29.538823398837614
26-27	24.36128933113066	22.608543462206683	26.074976201757867	26.955191004904787
28-29	24.677331568893205	23.548872501414042	24.732826056796476	27.04096987289628
30-31	24.32978160271844	23.018586821030343	24.97118487782388	27.680446698427335
32-33	24.207756061223748	23.126884961078822	25.298018616425228	27.367340361272202
34-35	24.773270097266007	22.77971180916482	25.169420438027874	27.277597655541303
36-37	25.050587411632115	20.581605819473378	28.98936170212766	25.378445066766847
38-39	24.66851371196339	19.790350397868924	32.0649482599638	23.47618763020389
40-41	24.39492933816294	21.844870533341588	24.935378730797716	28.824821397697754
42-43	22.43331156274412	21.856177511408216	26.302073328466967	29.408437597380697
44-45	22.085046254199593	20.783012947760714	28.671627200116117	28.460313597923577
46-47	23.627193385000567	20.154489435680272	29.359182601902624	26.859134577416537
48-49	23.64729587241145	20.8353788425335	29.362270707312177	26.15505457774287
50-51	24.53128201625572	19.83599139403046	31.238474147940714	24.394252441773105
52-53	20.51744526077175	22.523660922018212	28.65970535375053	28.29918846345951
54-55	22.19663222333452	21.66173246901274	28.02289866146993	28.118736646182807
56-57	21.970908081918402	22.215724149707054	27.594847550238523	28.21852021813602
58-59	20.697670447511427	22.867565133126433	27.852602550255924	28.582161869106216
60-61	20.84166668445397	22.015628635279903	27.47369791611081	29.669006764155313
62-63	20.719272744348466	22.79141346549954	27.10325058004145	29.386063210110546
64-65	21.467493964948677	22.96222303329897	26.153596591770135	29.41668640998222
66-67	20.34024205425943	23.78023010096267	22.724497854810135	33.15502998996777
68-69	20.62346945529155	24.237209610166126	22.859265680143643	32.28005525439868
70-71	20.550907150480256	24.804695837780148	22.37545357524013	32.26894343649946
72-73	19.844569760045765	24.993276500194234	21.314134717591664	33.848019022168344
74-75	21.09043330892978	25.566955738499203	24.686079942626748	28.65653100994427
76	21.526390383006337	25.027321004729945	25.55837303416833	27.887915578095384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	1.0
3	1.5
4	3.0
5	2.0
6	0.5
7	1.0
8	2.0
9	1.5
10	1.0
11	1.5
12	2.0
13	2.5
14	2.5
15	2.5
16	3.0
17	3.0
18	10.5
19	22.0
20	29.5
21	33.0
22	49.5
23	105.5
24	197.5
25	250.0
26	336.5
27	501.5
28	713.5
29	847.0
30	1015.5
31	1405.5
32	1982.0
33	2337.0
34	2677.5
35	3586.0
36	4660.0
37	5166.0
38	5785.0
39	7131.0
40	8676.0
41	10028.5
42	10563.0
43	11090.0
44	11992.5
45	12653.5
46	12939.0
47	12847.5
48	12487.5
49	11825.0
50	11431.0
51	10830.0
52	9595.0
53	8299.5
54	7638.0
55	7199.0
56	6476.0
57	5890.5
58	5589.0
59	5347.0
60	5037.0
61	4794.5
62	4620.0
63	4518.5
64	4461.0
65	4453.0
66	4343.5
67	4286.0
68	4221.0
69	4075.0
70	3869.0
71	3744.0
72	3665.0
73	3338.0
74	2909.0
75	2728.0
76	2483.0
77	1997.5
78	1526.0
79	1295.0
80	1113.0
81	769.0
82	505.0
83	403.0
84	308.5
85	167.0
86	97.5
87	75.0
88	67.0
89	44.0
90	19.5
91	8.0
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.2685607693653937E-4
4	0.0017074243077461575
5	0.0
6	0.0
7	0.0
8	0.004695416846301932
9	0.005549129000175011
10-11	0.007683409384857708
12-13	0.00682969723098463
14-15	0.005549129000175011
16-17	0.010671401923413483
18-19	0.008750549577199056
20-21	0.005549129000175011
22-23	0.008963977615667324
24-25	0.004908844884770202
26-27	0.004268560769365393
28-29	0.005762557038643281
30-31	0.008110265461794247
32-33	0.006616269192516359
34-35	0.007043125269452899
36-37	0.009817689769540405
38-39	0.009817689769540405
40-41	0.009177405654135596
42-43	0.004268560769365393
44-45	0.009390833692603864
46-47	0.006616269192516359
48-49	0.007683409384857708
50-51	0.00640284115404809
52-53	0.010244545846476943
54-55	0.009177405654135596
56-57	0.005762557038643281
58-59	0.008537121538730786
60-61	0.009177405654135596
62-63	0.008750549577199056
64-65	0.00618941311557982
66-67	0.011098258000350022
68-69	0.03478877027032795
70-71	0.008963977615667324
72-73	0.007683409384857708
74-75	0.006616269192516359
76	0.008110265461794247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	234271.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.3796571839404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.03168717908764	92.54849059120723
2	2.097395799414771	4.000977846544349
3	0.4607193277417996	1.3182975461408497
4	0.18722700375938775	0.7143058973658689
5	0.09461197768780846	0.4512028998678894
6	0.04905736303900364	0.28074446814049653
7	0.027562444538509936	0.18402275578641134
8	0.018375167475424784	0.1402093739602806
9	0.01115316487562573	0.09574065381178325
>10	0.02221057238000586	0.26600796717485714
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0017074243077461573	0.0
2	0.0	0.0	0.0	0.0021342803846826964	0.0
3	0.0	0.0	0.0	0.0021342803846826964	0.0
4	0.0	0.0	0.0	0.0025611364616192358	0.0
5	0.0	0.0	0.0	0.0034148486154923145	0.0
6	0.0	0.0	0.0	0.0034148486154923145	0.0
7	0.0	0.0	0.0	0.004695416846301932	0.0
8	0.0	0.0	0.0	0.004695416846301932	0.0
9	0.0	0.0	0.0	0.00597598507711155	0.0
10	0.0	0.0	0.0	0.007256553307921168	0.0
11	0.0	0.0	0.0	0.007256553307921168	0.0
12	0.0	0.0	0.0	0.007683409384857708	0.0
13	0.0	0.0	0.0	0.008110265461794247	0.0
14	0.0	0.0	0.0	0.008537121538730786	0.0
15	0.0	0.0	0.0	0.008963977615667326	0.0
16	0.0	0.0	0.0	0.009390833692603864	0.0
17	0.0	0.0	0.0	0.010671401923413483	0.0
18	0.0	0.0	0.0	0.011098258000350022	0.0
19	0.0	0.0	0.0	0.013232538385032718	0.0
20	0.0	0.0	0.0	0.015366818769715416	0.0
21	0.0	0.0	0.0	0.01835481130827119	0.0
22	0.0	0.0	0.0	0.026465076770065436	0.0
23	0.0	0.0	0.0	0.029453069308621214	0.0
24	0.0	0.0	0.0	0.034575342231859686	0.0
25	0.0	0.0	0.0	0.04012447123203469	0.0
26	0.0	0.0	0.0	0.04183189553978085	0.0
27	0.0	0.0	0.0	0.08963977615667325	0.0
28	0.0	0.0	0.0	0.13958193715824835	0.0
29	0.0	0.0	0.0	0.18397496915964845	0.0
30	0.0	0.0	0.0	0.2369051226997793	0.0
31	0.0	0.0	0.0	0.26806561631614667	0.0
32	0.0	0.0	0.0	0.32483747454870643	0.0
33	0.0	0.0	0.0	0.38288990101207576	0.0
34	0.0	0.0	0.0	0.44905259293723937	0.0
35	0.0	0.0	0.0	0.6082699096345685	0.0
36	0.0	0.0	0.0	0.7508398393313727	0.0
37	0.0	0.0	0.0	1.1533651198825292	0.0
38	0.0	0.0	0.0	1.5780869164343858	0.0
39	0.0	0.0	0.0	2.1065347396818215	0.0
40	0.0	0.0	0.0	2.7271834755475495	0.0
41	0.0	0.0	0.0	3.5164403618032107	0.0
42	0.0	0.0	0.0	4.320637210751651	0.0
43	0.0	0.0	0.0	5.226852662087924	0.0
44	0.0	0.0	0.0	6.0033038660354885	4.268560769365393E-4
45	0.0	0.0	0.0	6.569741880130277	4.268560769365393E-4
46	0.0	0.0	0.0	7.245455049920818	4.268560769365393E-4
47	0.0	0.0	0.0	8.05562788394637	8.537121538730786E-4
48	0.0	0.0	0.0	8.600723094194331	8.537121538730786E-4
49	0.0	0.0	0.0	9.076667619978572	8.537121538730786E-4
50	0.0	0.0	0.0	9.755795638384607	8.537121538730786E-4
51	0.0	0.0	0.0	10.56084619948692	8.537121538730786E-4
52	0.0	0.0	0.0	11.301014636894879	8.537121538730786E-4
53	0.0	0.0	0.0	12.294735584003142	8.537121538730786E-4
54	0.0	0.0	0.0	13.12753179010633	8.537121538730786E-4
55	0.0	0.0	0.0	13.635917377737748	8.537121538730786E-4
56	0.0	0.0	0.0	14.17717088329328	8.537121538730786E-4
57	0.0	0.0	0.0	14.658664538077696	8.537121538730786E-4
58	0.0	0.0	0.0	15.116254252553667	8.537121538730786E-4
59	0.0	0.0	0.0	15.608419309261496	8.537121538730786E-4
60	0.0	0.0	0.0	15.949050458656854	8.537121538730786E-4
61	0.0	0.0	0.0	16.283705622975102	8.537121538730786E-4
62	0.0	0.0	0.0	16.544514685983327	8.537121538730786E-4
63	0.0	0.0	0.0	16.868071592301224	8.537121538730786E-4
64	0.0	0.0	0.0	17.21852043146612	8.537121538730786E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	20	0.0069469237	52.496635	24
ATGGAAT	40	7.4618583E-7	52.496635	22
CGTTAGT	20	0.0069469237	52.496635	37
GCCGTAT	115	0.0	51.73582	54
GAGTGAC	30	5.8716215E-4	46.66368	7
TAGATCT	190	0.0	44.207695	39
TTCGTGT	40	4.58529E-5	43.747196	33
GTAGATC	145	0.0	43.445496	38
ACGCTTC	115	0.0	42.60597	15
CTCTCGG	75	7.530616E-10	41.99731	42
ATCTCGT	100	0.0	41.997307	42
GACGCTT	100	0.0	41.997307	14
TGGTCGC	170	0.0	41.173836	49
GTAATGG	35	0.0012541183	39.997437	58
GCCGAAA	80	1.4151738E-9	39.37248	70
CTTCCGA	125	0.0	39.19749	18
CGCCGTA	165	0.0	38.179375	53
AGATCTC	225	0.0	37.33094	40
TCGGTGG	240	0.0	36.456	45
CGACACT	50	1.7005613E-4	35.020184	68
>>END_MODULE