FastQCFastQC Report
Wed 25 May 2016
SRR1779753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234271
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2382510.16984603301305No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG26681.1388520132666868No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC16460.7026051026375437No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT10270.4383811910138259TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC6870.2932501248554025No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC6520.2783101621626236No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT4390.18738981777514074No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC4310.18397496915964845No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2760.11781227723448484No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2480.10586030708026174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGT150.002222687570.08
GGTAACC200.006945164452.519
CCCTTTA200.006945164452.515
TGTAGCG352.0823405E-550.00000416
CGTCTTC34950.049.87124348
CCGTCTT34900.049.8424147
TCTTCTG35000.049.750
CTCGTAT35150.049.6870639
TATGCCG35300.049.6742243
TCTCGTA33750.049.67407238
CGTATGC35250.049.6453941
TCGTATG35250.049.6453940
GCCGTCT35200.049.61647446
TCTTTAT1200.049.5833321
CTTTATA1200.049.5833322
ATCTCGT33800.049.49704437
GTCTTCT35150.049.48791549
ATGCCGT35450.049.36530344
CTTGAAA35150.049.2887757
TGCCGTC35550.049.22643745
Produced by FastQC (version 0.11.5)