##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779753_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234271
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.66321482385784	34.0	34.0	34.0	34.0	34.0
2	33.655557025837595	34.0	34.0	34.0	34.0	34.0
3	33.682098936701514	34.0	34.0	34.0	34.0	34.0
4	33.71325516175711	34.0	34.0	34.0	34.0	34.0
5	33.71015618663855	34.0	34.0	34.0	34.0	34.0
6	37.367074883361575	38.0	38.0	38.0	37.0	38.0
7	37.360996452826	38.0	38.0	38.0	37.0	38.0
8	37.24776007273628	38.0	38.0	38.0	36.0	38.0
9	37.24723503976164	38.0	38.0	38.0	36.0	38.0
10-11	37.1735447409197	38.0	38.0	38.0	36.0	38.0
12-13	37.14034600953597	38.0	38.0	38.0	36.0	38.0
14-15	37.16023536844082	38.0	38.0	38.0	36.0	38.0
16-17	37.157462938221116	38.0	38.0	38.0	36.0	38.0
18-19	37.15415266934448	38.0	38.0	38.0	36.0	38.0
20-21	37.15529877791105	38.0	38.0	38.0	36.0	38.0
22-23	37.108338206606874	38.0	38.0	38.0	36.0	38.0
24-25	37.098838524614656	38.0	38.0	38.0	36.0	38.0
26-27	37.019701542231005	38.0	38.0	38.0	35.5	38.0
28-29	36.963164454840765	38.0	38.0	38.0	35.5	38.0
30-31	36.75382569758955	38.0	37.0	38.0	34.0	38.0
32-33	36.798363860657105	38.0	37.0	38.0	34.5	38.0
34-35	36.846545240341314	38.0	38.0	38.0	35.0	38.0
36-37	36.83842857203837	38.0	37.0	38.0	35.0	38.0
38-39	36.39880096127989	38.0	37.0	38.0	34.0	38.0
40-41	36.78735524243291	38.0	37.0	38.0	34.5	38.0
42-43	36.92042549013749	38.0	37.0	38.0	35.0	38.0
44-45	36.90686213829284	38.0	38.0	38.0	35.0	38.0
46-47	36.94269884023204	38.0	38.0	38.0	35.0	38.0
48-49	36.925421840518034	38.0	38.0	38.0	35.0	38.0
50-51	36.867034332034265	38.0	37.5	38.0	35.0	38.0
52-53	36.85574185451891	38.0	38.0	38.0	35.0	38.0
54-55	36.85067507288568	38.0	38.0	38.0	35.0	38.0
56-57	36.88112485113395	38.0	38.0	38.0	35.0	38.0
58-59	36.80750711782508	38.0	37.0	38.0	35.0	38.0
60-61	36.86563210982153	38.0	37.5	38.0	35.0	38.0
62-63	36.875328145609146	38.0	38.0	38.0	35.0	38.0
64-65	36.895162013224	38.0	38.0	38.0	35.0	38.0
66-67	36.84333314836237	38.0	38.0	38.0	35.0	38.0
68-69	36.697348370050065	38.0	37.0	38.0	34.0	38.0
70-71	35.79753575986784	38.0	37.0	38.0	30.5	38.0
72-73	34.267672908725366	38.0	36.5	38.0	22.0	38.0
74-75	34.09575662373918	38.0	36.0	38.0	20.0	38.0
76	33.61850164979874	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	7.0
22	18.0
23	40.0
24	71.0
25	151.0
26	263.0
27	428.0
28	761.0
29	1309.0
30	2025.0
31	3044.0
32	4901.0
33	7912.0
34	13422.0
35	26551.0
36	35981.0
37	137379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.85274319057843	15.979357240119349	10.51517259925471	35.65272697004751
2	19.683187419697703	15.93112250342552	39.07312471454	25.31256536233678
3	18.770142271130442	17.961250005335703	35.24166456795762	28.026943155576234
4	14.14131497283061	23.89156148221504	31.089635507595904	30.877488037358443
5	14.460603318379142	30.165492101028295	39.815854288409575	15.558050292182985
6	29.202077935382526	40.05489369149404	15.289984675866839	15.453043697256597
7	27.58173226733142	25.047060882482253	17.831912614023928	29.539294236162394
8	24.224936078302477	39.09788236700232	15.73263442765003	20.944547127045173
9	22.265239829086827	15.09491144870684	16.26791194812845	46.37193677407788
10-11	24.952939117517747	26.062764917552748	26.093925411169117	22.890370553760388
12-13	24.19847100153241	18.01674129533745	26.848606955192917	30.936180747937218
14-15	22.505773228440567	18.48222784723675	19.17245412364313	39.839544800679555
16-17	29.434714497312942	26.133409598285745	19.360911081610613	25.0709648227907
18-19	29.32287820515557	21.620900580951123	19.957655877167895	29.098565336725418
20-21	22.858783204067084	20.65428499472833	19.982626957668685	36.504304843535905
22-23	23.35052140469798	26.715214431150248	19.78029717720076	30.15396698695101
24-25	29.239427842114473	26.669327402879574	19.63153783438838	24.459706920617574
26-27	28.962184820144195	27.61673446564022	20.376828544719576	23.04425216949601
28-29	28.9325183227971	21.685782704645476	20.617575372111784	28.76412360044564
30-31	29.616982042164842	26.26744240644382	20.261577403946713	23.853998147444628
32-33	23.542393211280952	26.807628771807007	20.365730286719227	29.28424773019281
34-35	28.211131552774347	21.549829044141187	21.102270447473227	29.136768955611238
36-37	23.752192973095262	25.535384234497656	27.04922931135309	23.663193481053995
38-39	21.75173196853217	21.5651958629109	28.0884104306551	28.594661737901834
40-41	21.953848320961622	19.269990737223132	28.80680920813929	29.969351733675957
42-43	27.760158107490895	19.22196942856777	28.276013676468704	24.74185878747263
44-45	21.562634726449286	25.050689159136212	29.93285553909788	23.45382057531662
46-47	26.93846015938806	19.34063541795613	24.638132760777047	29.082771661878766
48-49	26.256984432558873	18.773770547784405	23.971170140563704	30.998074879093018
50-51	20.50253765937739	19.456953698921335	30.117043936296	29.92346470540528
52-53	18.996589419945277	19.76343636216177	36.07830247875324	25.161671739139713
54-55	18.483508415467558	19.780083749162294	31.06402414297971	30.672383692390436
56-57	24.250974298995608	19.858411839280148	24.959341958671793	30.931271903052448
58-59	19.329110303878842	19.924147675128374	34.62186954424576	26.12487247674701
60-61	24.64304160566182	26.220274809942335	23.746857272133553	25.389826312262294
62-63	18.81859043586274	34.042839275881356	21.429455630445084	25.70911465781083
64-65	18.737274353206328	35.02311425656611	20.159558801558877	26.08005258866868
66-67	18.78508223382322	35.04893904922077	19.324201458994068	26.841777257961937
68-69	18.76309914586099	35.399387888385675	19.40573096968895	26.431781996064384
70-71	19.631964690465313	34.2005625963094	19.3192926141093	26.848180099115982
72-73	20.425916993567277	31.093477212288334	19.57348540792501	28.907120386219376
74-75	21.06961595758758	29.88013881359622	20.01848286813135	29.031762360684848
76	21.567330143295585	29.915354439943485	19.217060583683	29.300254833077933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.0
17	6.0
18	8.0
19	21.0
20	40.5
21	49.0
22	75.5
23	126.5
24	231.5
25	312.0
26	388.5
27	520.0
28	685.0
29	795.0
30	993.0
31	1331.5
32	1790.0
33	2108.0
34	2414.5
35	3135.5
36	4040.0
37	4530.0
38	5079.0
39	6356.5
40	7676.5
41	13396.5
42	18525.0
43	19077.5
44	18748.0
45	15771.5
46	13677.0
47	12676.0
48	10876.0
49	9477.0
50	8877.0
51	8549.0
52	7580.5
53	6425.0
54	5910.0
55	5482.0
56	4754.0
57	4146.5
58	3839.0
59	3711.5
60	3667.0
61	3679.0
62	3608.0
63	3696.5
64	3865.5
65	4074.5
66	4084.5
67	3966.0
68	4015.5
69	3982.5
70	3965.5
71	4031.0
72	3886.0
73	3551.5
74	3248.5
75	3135.0
76	2957.0
77	2602.0
78	2147.0
79	1869.0
80	1691.0
81	1287.0
82	928.0
83	795.0
84	594.0
85	341.0
86	204.0
87	119.0
88	92.5
89	54.5
90	34.0
91	19.0
92	13.0
93	10.0
94	4.5
95	1.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	234271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.42966052136202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.224475203023644	15.118388532938349
2	24.088308902395426	16.10527978281563
3	13.548955513560449	13.588109497120856
4	6.818530057714899	9.11764580336448
5	3.303284130956637	5.521383355174136
6	1.7927371162980745	3.595835592113407
7	1.0278870218090812	2.405333993537399
8	0.7418662853056847	1.9840270456010347
9	0.5158588283364829	1.552048695741257
>10	2.8768067827774657	16.074973001353136
>50	0.03958322692680934	0.8302350696415689
>100	0.014045661167577507	1.0854950036496196
>500	0.002553756575923183	0.5715602870180261
>1k	0.0038306348638847748	2.2798383069180566
>5k	0.0	0.0
>10k+	0.0012768782879615916	10.16984603301305
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	23825	10.16984603301305	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	2668	1.1388520132666868	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	1646	0.7026051026375437	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	1027	0.4383811910138259	TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	687	0.2932501248554025	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	652	0.2783101621626236	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT	439	0.18738981777514074	No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	431	0.18397496915964845	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	276	0.11781227723448484	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	248	0.10586030708026174	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0017074243077461573	0.0
2	0.0	0.0	0.0	0.0021342803846826964	0.0
3	0.0	0.0	0.0	0.0021342803846826964	0.0
4	0.0	0.0	0.0	0.002987992538555775	0.0
5	0.0	0.0	0.0	0.0051222729232384715	0.0
6	0.0	0.0	0.0	0.0051222729232384715	0.0
7	0.0	0.0	0.0	0.00640284115404809	0.0
8	0.0	0.0	0.0	0.006829697230984629	0.0
9	0.0	0.0	0.0	0.008537121538730786	0.0
10	0.0	0.0	0.0	0.01280568230809618	0.0
11	0.0	0.0	0.0	0.01280568230809618	0.0
12	0.0	0.0	0.0	0.013232538385032718	0.0
13	0.0	0.0	0.0	0.014086250538905797	0.0
14	0.0	0.0	0.0	0.015366818769715416	0.0
15	0.0	0.0	0.0	0.016220530923588494	0.0
16	0.0	0.0	0.0	0.016647387000525034	0.0
17	0.0	0.0	0.0	0.01835481130827119	0.0
18	0.0	0.0	0.0	0.021769659923763503	0.0
19	0.0	0.0	0.0	0.0239039403084462	0.0
20	0.0	0.0	0.0	0.027318788923938516	0.0
21	0.0	0.0	0.0	0.033721630077986606	0.0
22	0.0	0.0	0.0	0.03841704692428854	0.0
23	0.0	0.0	0.0	0.04183189553978085	0.0
24	0.0	0.0	0.0	0.04396617592446355	0.0
25	0.0	0.0	0.0	0.04866159277076548	0.0
26	0.0	0.0	0.0	0.05122272923238472	0.0
27	0.0	0.0	0.0	0.11482428469592908	0.0
28	0.0	0.0	0.0	0.17885269623640998	0.0
29	0.0	0.0	0.0	0.2693461845469563	0.0
30	0.0	0.0	0.0	0.3470339905494065	0.0
31	0.0	0.0	0.0	0.43966175924463546	0.0
32	0.0	0.0	0.0	0.542107217709405	0.0
33	0.0	0.0	0.0	0.668883472559557	0.0
34	0.0	0.0	0.0	0.8225516602567112	0.0
35	0.0	0.0	0.0	1.0547613661101887	0.0
36	0.0	0.0	0.0	1.4474689568918049	0.0
37	0.0	0.0	0.0	2.0489091692953885	0.0
38	0.0	0.0	0.0	2.797187872165142	0.0
39	0.0	0.0	0.0	3.605226425806011	0.0
40	0.0	0.0	0.0	4.499916763064998	0.0
41	0.0	0.0	0.0	5.563642106790853	0.0
42	0.0	0.0	0.0	6.712311809827081	0.0
43	0.0	0.0	0.0	7.963426971328078	0.0
44	0.0	0.0	0.0	9.026725458976996	0.0
45	0.0	0.0	0.0	10.113501030857426	0.0
46	0.0	0.0	0.0	11.233571376738904	0.0
47	0.0	0.0	0.0	12.342116608543098	0.0
48	0.0	0.0	0.0	13.356326647344314	0.0
49	0.0	0.0	0.0	14.297544296989384	0.0
50	0.0	0.0	0.0	15.148268458323907	0.0
51	0.0	0.0	0.0	16.108267775354182	0.0
52	0.0	0.0	0.0	17.131441791771067	0.0
53	0.0	0.0	0.0	18.222059068343928	0.0
54	0.0	0.0	0.0	19.16413043014287	0.0
55	0.0	0.0	0.0	20.052417926247806	0.0
56	0.0	0.0	0.0	20.861737048119487	0.0
57	0.0	0.0	0.0	21.682154427991513	0.0
58	0.0	0.0	0.0	22.47696044324735	0.0
59	0.0	0.0	0.0	23.197493501116227	0.0
60	0.0	0.0	0.0	23.944064779678236	0.0
61	0.0	0.0	0.0	24.684233217086195	0.0
62	0.0	0.0	0.0	25.455989004187458	0.0
63	0.0	0.0	0.0	26.153044977824827	0.0
64	0.0	0.0	0.0	26.946143568772918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTCGT	15	0.0022226875	70.0	8
GGTAACC	20	0.0069451644	52.5	19
CCCTTTA	20	0.0069451644	52.5	15
TGTAGCG	35	2.0823405E-5	50.000004	16
CGTCTTC	3495	0.0	49.871243	48
CCGTCTT	3490	0.0	49.84241	47
TCTTCTG	3500	0.0	49.7	50
CTCGTAT	3515	0.0	49.68706	39
TATGCCG	3530	0.0	49.67422	43
TCTCGTA	3375	0.0	49.674072	38
CGTATGC	3525	0.0	49.64539	41
TCGTATG	3525	0.0	49.64539	40
GCCGTCT	3520	0.0	49.616474	46
TCTTTAT	120	0.0	49.583332	1
CTTTATA	120	0.0	49.583332	2
ATCTCGT	3380	0.0	49.497044	37
GTCTTCT	3515	0.0	49.487915	49
ATGCCGT	3545	0.0	49.365303	44
CTTGAAA	3515	0.0	49.28877	57
TGCCGTC	3555	0.0	49.226437	45
>>END_MODULE