FastQCFastQC Report
Wed 25 May 2016
SRR1779752_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779752_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122432
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC1350.057.0300540
GTAGATC950.055.25638638
CTCGGTG1300.053.8395544
TAGATCT1300.053.8395539
ACTCTGC608.367351E-1152.4935715
GCGCACA200.006940644252.49356536
ACGCTAA200.006940644252.49356523
TATCGTA200.006940644252.49356550
GTGAAAC200.006940644252.49356548
ATCTCGT552.2610038E-950.9028542
GATCTCG1450.050.6834441
TCTCGGT1500.048.99399643
TTAGATC506.146547E-848.9939938
GATCTCT506.146547E-848.9939941
CGGTGGT1100.047.72142446
TCGGTGG1400.047.4941845
ATCTCGG1350.046.6609542
TAGATCC305.8622647E-446.6609539
TGTCGAT451.6747981E-646.6609537
TGTCCGC305.8622647E-446.6609546
Produced by FastQC (version 0.11.5)