##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779752_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	122432
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.440072856769472	33.0	23.0	34.0	12.0	34.0
2	27.271865198640878	32.0	23.0	34.0	12.0	34.0
3	26.2458180867747	32.0	21.0	34.0	11.0	34.0
4	28.206473797699946	33.0	23.0	34.0	12.0	34.0
5	27.834316191845268	33.0	23.0	34.0	11.0	34.0
6	30.028456612650288	35.0	25.0	38.0	11.0	38.0
7	30.97225398588604	36.0	26.0	38.0	12.0	38.0
8	30.2252924072138	36.0	25.0	38.0	11.0	38.0
9	31.66537343178254	37.0	27.0	38.0	12.0	38.0
10-11	31.258175967067434	37.0	27.0	38.0	11.0	38.0
12-13	30.512676424464193	35.5	25.5	38.0	11.0	38.0
14-15	30.337027901202298	35.0	25.0	38.0	11.0	38.0
16-17	30.112944328280186	35.5	24.0	38.0	11.0	38.0
18-19	30.707829652378464	36.0	26.0	38.0	11.5	38.0
20-21	29.595885879508625	35.0	23.5	38.0	11.0	38.0
22-23	29.591226150026138	35.5	24.0	38.0	11.0	38.0
24-25	30.607819034239416	36.0	25.0	38.0	11.0	38.0
26-27	29.28626094485102	34.5	22.0	38.0	11.0	38.0
28-29	31.137521236278097	37.0	26.5	38.0	11.0	38.0
30-31	31.61939688970204	37.0	27.0	38.0	11.0	38.0
32-33	31.740402835859904	37.0	27.0	38.0	11.0	38.0
34-35	31.754034892838476	37.0	27.0	38.0	11.0	38.0
36-37	29.930692139309983	35.0	24.0	38.0	11.0	38.0
38-39	28.420792766596968	34.0	21.0	37.5	11.0	38.0
40-41	29.304850855985364	34.0	22.5	37.5	11.0	38.0
42-43	30.280833442237324	35.5	25.0	38.0	11.0	38.0
44-45	31.830481410088865	37.0	27.0	38.0	11.0	38.0
46-47	30.562414238107685	35.5	26.5	38.0	11.0	38.0
48-49	29.952030514898066	35.5	23.5	38.0	11.0	38.0
50-51	27.989100071876635	33.5	21.0	37.5	11.0	38.0
52-53	30.938815015682174	36.5	26.0	38.0	11.0	38.0
54-55	30.108476542080503	35.5	24.0	38.0	11.0	38.0
56-57	30.538837885520124	36.0	25.0	38.0	11.0	38.0
58-59	31.525054724255096	37.0	27.0	38.0	11.0	38.0
60-61	32.0001919432828	37.0	30.5	38.0	11.0	38.0
62-63	31.932934200209097	37.0	29.0	38.0	11.0	38.0
64-65	31.682799431521172	37.0	29.0	38.0	11.0	38.0
66-67	29.772865754051228	35.5	23.5	38.0	11.0	38.0
68-69	29.828468047569263	35.0	23.5	38.0	11.0	38.0
70-71	29.469436095138526	35.0	23.0	38.0	11.0	38.0
72-73	28.385769243335076	34.0	21.5	37.5	11.0	38.0
74-75	30.192498693152118	35.5	24.0	38.0	11.0	38.0
76	30.55866930214323	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	0.0
5	2.0
6	1.0
7	0.0
8	1.0
9	1.0
10	0.0
11	11.0
12	46.0
13	262.0
14	691.0
15	1278.0
16	2055.0
17	3328.0
18	4339.0
19	4800.0
20	4086.0
21	3379.0
22	2646.0
23	2172.0
24	2064.0
25	2108.0
26	2202.0
27	2472.0
28	2837.0
29	3417.0
30	4185.0
31	5095.0
32	6659.0
33	8768.0
34	11841.0
35	15238.0
36	17124.0
37	9320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.05674986931521	18.54988891792995	12.599647151071617	32.79371406168322
2	19.066093831677993	15.879835337166753	42.26264375326712	22.791427077888134
3	19.02867737746159	18.15798286381717	37.99282861366811	24.820511145053132
4	14.204735728696164	19.604511929168268	31.99679819653519	34.19395414560038
5	13.961219289074753	28.019635389440666	39.49457658128594	18.524568740198642
6	26.147575797177208	33.95599189754313	16.6288225300575	23.267609775222166
7	25.846184004181914	24.48869576581286	21.411069001568215	28.25405122843701
8	21.815449206465896	32.38828036299182	18.662549927712025	27.133720502830254
9	22.07800694302634	15.92648560343067	20.211558096793954	41.78394935674903
10-11	24.319368092596978	23.28402342697042	27.305326613463155	25.09128186696944
12-13	23.19193948898909	18.738564333790762	29.95654446840489	28.112951708815263
14-15	23.93414770615599	22.065664424486727	23.60578474255772	30.394403126799563
16-17	25.319898873972303	21.784572154400983	25.742210314364716	27.153318657262
18-19	21.935649872574007	21.452492975233614	28.250588119976477	28.36126903221591
20-21	25.05319561692621	21.20309902757187	26.853065742022697	26.890639613479216
22-23	26.28539455251647	20.25509808165916	26.70524858380948	26.75425878201489
24-25	22.315929622792545	24.09741394801758	23.72535245781125	29.86130397137863
26-27	24.134579256040382	23.477039191021515	26.59320732524137	25.795174227696734
28-29	23.26598625286399	24.12691800319378	25.81610857215204	26.790987171790192
30-31	22.75185218463851	23.868880847553157	26.09885397351805	27.280412994290288
32-33	22.86819330940041	23.689100718396087	26.30865301754945	27.134052954654052
34-35	23.045632205971845	23.633749780476947	26.167964745618725	27.15265326793248
36-37	25.239744490369375	20.445263106304424	30.084053519792192	24.230938883534005
38-39	24.40809491719251	19.576058322612262	33.80710245257204	22.208744307623192
40-41	23.626279375607524	22.994453656584138	25.706362366548767	27.672904601259567
42-43	20.344534657670756	23.691862839592897	27.3124172969794	28.651185205756946
44-45	20.581099956707483	21.525775385344257	29.855909428783807	28.037215229164453
46-47	23.716034911597855	20.581015899725134	29.442468153581626	26.260481035095385
48-49	23.735521393213638	21.20493048635049	30.10284099263204	24.956707127803828
50-51	26.99530324688585	19.753318358178475	30.44639575250153	22.804982642434144
52-53	19.53620205188525	23.284241651963665	29.56568973403908	27.613866562112005
54-55	22.72712420970087	21.862900459067813	28.142817467448662	27.267157863782653
56-57	22.833163161119053	22.006534613028382	27.642638350010213	27.517663875842352
58-59	20.010373782918382	23.482323727373718	28.490737110370517	28.016565379337383
60-61	19.892176683207744	22.388449835610285	28.388980783761152	29.330392697420816
62-63	19.988074136395937	23.488641840177092	28.101337167035606	28.421946856391365
64-65	20.12497447416786	24.257300387992647	27.21748008985093	28.400245047988566
66-67	18.844983561028407	26.339520921399252	23.110539320794786	31.70495619677755
68-69	19.341383902674174	26.411232668534968	23.451096059415164	30.7962873693757
70-71	18.915386594674956	27.265635529706795	22.818704230874477	31.000273644743775
72-73	18.11327844213553	27.348804156047834	22.111269032215905	32.42664836960073
74-75	19.708881800619974	26.62416428084248	25.724017659863836	27.94293625867371
76	20.00833169966673	25.247500490099977	27.3157224073711	27.428445402862184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	2.0
5	1.5
6	0.5
7	0.5
8	1.0
9	0.5
10	0.0
11	1.0
12	2.0
13	1.0
14	0.5
15	2.0
16	3.0
17	3.0
18	7.0
19	12.5
20	18.5
21	23.0
22	38.5
23	64.0
24	108.5
25	143.0
26	188.5
27	293.5
28	433.0
29	513.0
30	623.0
31	862.0
32	1190.5
33	1390.0
34	1624.0
35	2157.0
36	2739.0
37	3022.0
38	3434.0
39	4307.0
40	5198.5
41	5957.5
42	6286.0
43	6518.0
44	7002.5
45	7297.0
46	7339.0
47	7291.0
48	7002.5
49	6386.5
50	6011.0
51	5748.0
52	5107.0
53	4433.0
54	4137.0
55	3784.5
56	3322.0
57	3018.0
58	2824.0
59	2666.5
60	2396.5
61	2168.0
62	2052.0
63	2008.0
64	1930.5
65	1866.5
66	1759.5
67	1683.0
68	1647.5
69	1559.0
70	1466.0
71	1426.0
72	1345.5
73	1215.0
74	1054.0
75	943.0
76	872.0
77	713.0
78	536.0
79	447.0
80	390.0
81	274.0
82	165.5
83	116.0
84	97.0
85	60.5
86	32.5
87	22.0
88	16.5
89	6.5
90	3.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	8.167799268165185E-4
4	8.167799268165185E-4
5	0.0
6	0.0
7	0.0
8	0.004083899634082593
9	0.00571745948771563
10-11	0.0073510193413486675
12-13	0.006534239414532148
14-15	0.0053090695243073706
16-17	0.008576189231573446
18-19	0.006534239414532148
20-21	0.0053090695243073706
22-23	0.006942629377940408
24-25	0.004900679560899112
26-27	0.004900679560899112
28-29	0.0053090695243073706
30-31	0.0073510193413486675
32-33	0.0053090695243073706
34-35	0.006125849451123888
36-37	0.008167799268165186
38-39	0.0077594093047569255
40-41	0.0073510193413486675
42-43	0.004900679560899112
44-45	0.0073510193413486675
46-47	0.006942629377940408
48-49	0.008167799268165186
50-51	0.00571745948771563
52-53	0.006534239414532148
54-55	0.008167799268165186
56-57	0.00571745948771563
58-59	0.006534239414532148
60-61	0.0077594093047569255
62-63	0.0073510193413486675
64-65	0.00571745948771563
66-67	0.0077594093047569255
68-69	0.03185441714584423
70-71	0.008576189231573446
72-73	0.006534239414532148
74-75	0.006125849451123888
76	0.006534239414532148
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	122432.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.1418332179058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.96729897235497	90.34537454228344
2	2.8731618640690066	5.409694500304636
3	0.6527394131618665	1.8435025490591443
4	0.2539576018577543	0.9563213679404813
5	0.12492634039887977	0.5880397351177333
6	0.0589615151645668	0.3330447076138603
7	0.03597617920868153	0.23708044240168305
8	0.014990053619943496	0.11289529022529485
9	0.005996020191396664	0.05080286995466942
>10	0.011992039972921371	0.12324399509904961
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.167799268165185E-4	0.0
4	0.0	0.0	0.0	8.167799268165185E-4	0.0
5	0.0	0.0	0.0	0.003267119707266074	0.0
6	0.0	0.0	0.0	0.003267119707266074	0.0
7	0.0	0.0	0.0	0.004083899634082593	0.0
8	0.0	0.0	0.0	0.004900679560899112	0.0
9	0.0	0.0	0.0	0.006534239414532148	0.0
10	0.0	0.0	0.0	0.009801359121798223	0.0
11	0.0	0.0	0.0	0.009801359121798223	0.0
12	0.0	0.0	0.0	0.012251698902247778	0.0
13	0.0	0.0	0.0	0.013068478829064296	0.0
14	0.0	0.0	0.0	0.013068478829064296	0.0
15	0.0	0.0	0.0	0.013885258755880816	0.0
16	0.0	0.0	0.0	0.013885258755880816	0.0
17	0.0	0.0	0.0	0.014702038682697333	0.0
18	0.0	0.0	0.0	0.016335598536330372	0.0
19	0.0	0.0	0.0	0.018785938316779927	0.0
20	0.0	0.0	0.0	0.019602718243596447	0.0
21	0.0	0.0	0.0	0.025320177731312076	0.0
22	0.0	0.0	0.0	0.032671197072660745	0.0
23	0.0	0.0	0.0	0.03348797699947726	0.0
24	0.0	0.0	0.0	0.0351215368531103	0.0
25	0.0	0.0	0.0	0.037571876633559854	0.0
26	0.0	0.0	0.0	0.04002221641400941	0.0
27	0.0	0.0	0.0	0.05799137480397282	0.0
28	0.0	0.0	0.0	0.0963800313643492	0.0
29	0.0	0.0	0.0	0.11761630946157867	0.0
30	0.0	0.0	0.0	0.15273784631468898	0.0
31	0.0	0.0	0.0	0.17397412441191845	0.0
32	0.0	0.0	0.0	0.22379769994772608	0.0
33	0.0	0.0	0.0	0.27852195504443283	0.0
34	0.0	0.0	0.0	0.3634670674333508	0.0
35	0.0	0.0	0.0	0.5006860951385259	0.0
36	0.0	0.0	0.0	0.6134017250392054	0.0
37	0.0	0.0	0.0	0.9074424986931521	0.0
38	0.0	0.0	0.0	1.2268034500784109	0.0
39	0.0	0.0	0.0	1.6025222164140094	0.0
40	0.0	0.0	0.0	1.9937598013591218	0.0
41	0.0	0.0	0.0	2.433187401986409	0.0
42	0.0	0.0	0.0	2.945308416100366	0.0
43	0.0	0.0	0.0	3.4794824882383693	0.0
44	0.0	0.0	0.0	4.02835859905907	0.0
45	0.0	0.0	0.0	4.380390747516989	0.0
46	0.0	0.0	0.0	4.819818348144276	0.0
47	0.0	0.0	0.0	5.335206481965499	0.0
48	0.0	0.0	0.0	5.672536591740721	0.0
49	0.0	0.0	0.0	6.021301620491375	0.0
50	0.0	0.0	0.0	6.532605854678516	0.0
51	0.0	0.0	0.0	7.073314166231051	0.0
52	0.0	0.0	0.0	7.55929822268688	0.0
53	0.0	0.0	0.0	8.176783847360166	0.0
54	0.0	0.0	0.0	8.77221641400941	0.0
55	0.0	0.0	0.0	9.178156037637219	0.0
56	0.0	0.0	0.0	9.594713800313643	0.0
57	0.0	0.0	0.0	9.905906952430737	0.0
58	0.0	0.0	0.0	10.213016204913748	0.0
59	0.0	0.0	0.0	10.58546785154208	0.0
60	0.0	0.0	0.0	10.837852848928385	0.0
61	0.0	0.0	0.0	11.086970726607422	0.0
62	0.0	0.0	0.0	11.286265028750654	0.0
63	0.0	0.0	0.0	11.48882645060115	0.0
64	0.0	0.0	0.0	11.737944328280188	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCTC	135	0.0	57.03005	40
GTAGATC	95	0.0	55.256386	38
CTCGGTG	130	0.0	53.83955	44
TAGATCT	130	0.0	53.83955	39
ACTCTGC	60	8.367351E-11	52.49357	15
GCGCACA	20	0.0069406442	52.493565	36
ACGCTAA	20	0.0069406442	52.493565	23
TATCGTA	20	0.0069406442	52.493565	50
GTGAAAC	20	0.0069406442	52.493565	48
ATCTCGT	55	2.2610038E-9	50.90285	42
GATCTCG	145	0.0	50.68344	41
TCTCGGT	150	0.0	48.993996	43
TTAGATC	50	6.146547E-8	48.99399	38
GATCTCT	50	6.146547E-8	48.99399	41
CGGTGGT	110	0.0	47.721424	46
TCGGTGG	140	0.0	47.49418	45
ATCTCGG	135	0.0	46.66095	42
TAGATCC	30	5.8622647E-4	46.66095	39
TGTCGAT	45	1.6747981E-6	46.66095	37
TGTCCGC	30	5.8622647E-4	46.66095	46
>>END_MODULE