FastQCFastQC Report
Wed 25 May 2016
SRR1779752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122432
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1822514.885814166231052No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG19921.627025614218505No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC13061.066714584422373No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT8980.7334683742812337RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC5360.4377940407736539No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC4960.4051228437009932No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT4090.3340629900679561No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC3850.31446027182435965No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG2060.16825666492420283No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1820.14865394668060636No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT1730.1413029273392577No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1710.13966936748562467No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1600.13068478829064298No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1420.11598274960794563No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG1250.10209749085206482No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACT150.002220145470.08
TGCGCCA150.002220145470.056
CGATGGG150.002220145470.058
TTGCCGG150.002220145470.013
TCTTTAT850.065.8823551
CTTTATA950.058.9473652
ATCGTAT308.335632E-658.33333239
CTATACA700.055.01
TATATCG200.006937280852.517
TCTAGCA200.006937280852.53
GACCATA200.006937280852.557
ACGATGT200.006937280852.522
ACCTATG200.006937280852.563
TACGATG200.006937280852.521
CACCGTG200.006937280852.521
GATTATC200.006937280852.566
ATTTGGC200.006937280852.58
GCACTGG200.006937280852.51
GTACGAT200.006937280852.520
TCTCGTA25300.049.11067238
Produced by FastQC (version 0.11.5)