##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779752_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	122432
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.65708311552535	34.0	34.0	34.0	34.0	34.0
2	33.651275810245686	34.0	34.0	34.0	34.0	34.0
3	33.666574098274964	34.0	34.0	34.0	34.0	34.0
4	33.710966087297436	34.0	34.0	34.0	34.0	34.0
5	33.72079194981704	34.0	34.0	34.0	34.0	34.0
6	37.380325405122846	38.0	38.0	38.0	37.0	38.0
7	37.35710435180345	38.0	38.0	38.0	37.0	38.0
8	37.263558546785156	38.0	38.0	38.0	36.0	38.0
9	37.26068348144276	38.0	38.0	38.0	36.0	38.0
10-11	37.181100529273394	38.0	38.0	38.0	36.0	38.0
12-13	37.1535546262415	38.0	38.0	38.0	36.0	38.0
14-15	37.17178107030841	38.0	38.0	38.0	36.0	38.0
16-17	37.13017021693675	38.0	38.0	38.0	36.0	38.0
18-19	37.11864545216937	38.0	38.0	38.0	36.0	38.0
20-21	37.14901741374804	38.0	38.0	38.0	36.0	38.0
22-23	37.102432370622054	38.0	38.0	38.0	36.0	38.0
24-25	37.10556063774177	38.0	38.0	38.0	36.0	38.0
26-27	37.016756240198646	38.0	38.0	38.0	35.5	38.0
28-29	36.92402313120753	38.0	37.5	38.0	35.5	38.0
30-31	36.762705011761625	38.0	37.0	38.0	34.5	38.0
32-33	36.81440718112911	38.0	37.0	38.0	35.0	38.0
34-35	36.81675950731835	38.0	37.0	38.0	34.5	38.0
36-37	36.8206963865656	38.0	37.0	38.0	35.0	38.0
38-39	35.98749918322007	38.0	37.0	38.0	33.0	38.0
40-41	36.650834749085206	38.0	37.0	38.0	34.0	38.0
42-43	36.9406037637219	38.0	37.0	38.0	35.0	38.0
44-45	36.945859742550965	38.0	38.0	38.0	35.0	38.0
46-47	36.964196451907995	38.0	38.0	38.0	35.5	38.0
48-49	36.934584095661265	38.0	38.0	38.0	35.0	38.0
50-51	36.868657703868266	38.0	37.5	38.0	35.0	38.0
52-53	36.851395060115	38.0	37.5	38.0	35.0	38.0
54-55	36.85667554234187	38.0	38.0	38.0	35.0	38.0
56-57	36.86711398980658	38.0	37.0	38.0	35.0	38.0
58-59	36.7685368204391	38.0	37.0	38.0	35.0	38.0
60-61	36.8136026529012	38.0	37.0	38.0	35.0	38.0
62-63	36.85638150156822	38.0	37.5	38.0	35.0	38.0
64-65	36.88235101934135	38.0	38.0	38.0	35.0	38.0
66-67	36.80710108468374	38.0	38.0	38.0	35.0	38.0
68-69	36.5686585206482	38.0	37.0	38.0	34.0	38.0
70-71	35.20987568609514	38.0	36.0	38.0	28.0	38.0
72-73	32.947534141400936	38.0	33.5	38.0	16.0	38.0
74-75	32.65266842002091	38.0	34.0	38.0	11.0	38.0
76	32.32932566649242	38.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	10.0
22	7.0
23	15.0
24	38.0
25	69.0
26	129.0
27	239.0
28	407.0
29	661.0
30	1140.0
31	1840.0
32	2877.0
33	4504.0
34	7895.0
35	17074.0
36	19655.0
37	65870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.661395713538944	16.032573183481443	11.406331677992682	39.899699424986935
2	16.870589388395192	15.382416361735492	44.71706743335076	23.029926816518557
3	16.531625718766335	18.599712493465763	40.32850888656561	24.5401529012023
4	12.56534239414532	29.235820700470466	31.437042603240982	26.761794302143233
5	12.78914009409305	29.88271040250915	43.5474385781495	13.780710925248302
6	25.183775483533715	44.68276267642447	15.569458964976477	14.564002875065343
7	23.649862780972295	25.03675509670674	18.597262153685314	32.716119968635645
8	20.541198379508625	43.5376372190277	15.797340564558285	20.123823836905384
9	19.996406168322007	15.919040773653947	16.141204913748037	47.943348144276
10-11	21.857030841610037	28.58770582854156	27.750098013591217	21.805165316257188
12-13	21.424954260324096	17.604466152639834	29.331792341871406	31.63878724516466
14-15	20.39213604286461	17.885030057501304	19.08773849973863	42.63509539989545
16-17	29.707102718243593	27.895893230527967	18.413895060115003	23.983108991113433
18-19	30.202071353894404	21.506223863042344	18.61849843178254	29.67320635128071
20-21	20.559984317825407	20.039287114479876	18.74101542080502	40.6597131468897
22-23	21.1035513591218	28.765355462624147	18.819426293779404	31.311666884474647
24-25	29.69485101934135	28.976493073706223	18.669547177208575	22.659108729743856
26-27	29.360379639309986	30.97066126502875	19.07589519079979	20.593063904861474
28-29	21.05822007318348	30.212281102979617	20.060523392577103	28.668975431259803
30-31	20.589796785154206	30.530416884474647	28.27079521693675	20.608991113434396
32-33	37.44364218504966	20.847899242028227	21.82027574490329	19.88818282801882
34-35	23.504475953998956	28.018410219550443	28.999362911657084	19.477750914793518
36-37	29.18027966544694	29.23827104025091	20.937745033978043	20.643704260324096
38-39	20.23939819654992	21.482128855201253	29.296670805018294	28.981802143230528
40-41	19.692155645582854	18.75898457919498	31.093178254051228	30.455681521170934
42-43	28.50766139571354	18.941943282801883	30.119984971249348	22.430410350235235
44-45	19.51042211186618	28.03311225823314	31.486049398849975	20.970416231050706
46-47	28.131125849451124	19.236392446419238	23.386859644537374	29.245622059592264
48-49	27.164466806063775	18.66832200731835	22.028146236278097	32.13906495033978
50-51	19.39484775222164	18.700176424464193	31.473389309984316	30.43158651332985
52-53	17.477865263983272	18.93540904338735	40.38895060115003	23.197775091479354
54-55	17.348405645582854	19.014228306325144	33.0942890747517	30.543076973340305
56-57	26.136549268165187	18.914989545216937	24.266531625718766	30.681929560899114
58-59	18.343651986408783	19.70726607422896	38.46870099320439	23.480380946157865
60-61	26.160644276006273	28.868678123366443	23.131615917407213	21.839061683220073
62-63	17.53953214845792	40.216610036591746	20.09891204913748	22.14494576581286
64-65	16.846494380554102	41.7921785154208	19.14246275483534	22.218864349189754
66-67	17.11439819654992	41.60758625196027	18.72713016204914	22.55088538944067
68-69	17.015567825405125	42.09969615786722	18.636059200209097	22.248676816518557
70-71	17.49256730266597	40.123088734971255	19.24986931521171	23.13447464715107
72-73	18.27871798222687	34.8940636434919	20.8711774699425	25.956040904338735
74-75	18.689966675378987	32.97503920543649	22.141678646105593	26.193315473078933
76	18.833311552535285	33.54106769472033	21.863565081024568	25.762055671719814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	2.5
16	2.5
17	3.0
18	8.0
19	17.0
20	29.0
21	37.0
22	49.0
23	83.0
24	136.0
25	167.0
26	178.5
27	268.5
28	429.5
29	512.0
30	546.5
31	698.5
32	1003.5
33	1191.0
34	1425.0
35	1812.0
36	2114.5
37	2264.0
38	2783.5
39	7271.0
40	12036.0
41	11884.0
42	10935.0
43	9681.5
44	7770.5
45	6452.5
46	5792.0
47	5755.0
48	5271.5
49	4616.5
50	4408.0
51	4120.0
52	3398.0
53	2793.0
54	2622.0
55	2494.5
56	2225.5
57	1910.5
58	1737.0
59	1586.5
60	1451.0
61	1471.5
62	1477.0
63	1454.0
64	1498.0
65	1540.0
66	1476.5
67	1438.0
68	1443.0
69	1489.0
70	1484.0
71	1438.0
72	1368.5
73	1307.5
74	1245.5
75	1175.0
76	1054.5
77	842.0
78	713.5
79	677.0
80	587.0
81	416.5
82	292.0
83	248.0
84	202.0
85	113.0
86	57.0
87	44.0
88	35.5
89	21.0
90	10.0
91	3.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	122432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.686977260846838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.66593633200154	15.103894406691062
2	23.07256089702281	14.621994249869315
3	11.92421703827813	11.335271824359644
4	6.163165356360356	7.811683220073183
5	3.2169093955406627	5.0967067433350755
6	1.9151952571207629	3.6412049137480396
7	1.3558448253640933	3.007383690538421
8	1.0027065343472097	2.5418191322530057
9	0.6985436267560253	1.9921262415054888
>10	2.9282123985049617	13.676163094615786
>50	0.015465910555483955	0.3316126502875065
>100	0.028354169351720585	2.0893230527966544
>500	0.005155303518494652	1.1712624150548876
>1k	0.005155303518494652	2.693740198640878
>5k	0.0	0.0
>10k+	0.002577651759247326	14.885814166231052
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	18225	14.885814166231052	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	1992	1.627025614218505	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	1306	1.066714584422373	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	898	0.7334683742812337	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	536	0.4377940407736539	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	496	0.4051228437009932	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	409	0.3340629900679561	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	385	0.31446027182435965	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	206	0.16825666492420283	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	182	0.14865394668060636	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	173	0.1413029273392577	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	171	0.13966936748562467	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	160	0.13068478829064298	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT	142	0.11598274960794563	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG	125	0.10209749085206482	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.167799268165185E-4	0.0
2	0.0	0.0	0.0	8.167799268165185E-4	0.0
3	0.0	0.0	0.0	0.001633559853633037	0.0
4	0.0	0.0	0.0	0.003267119707266074	0.0
5	0.0	0.0	0.0	0.007351019341348667	0.0
6	0.0	0.0	0.0	0.007351019341348667	0.0
7	0.0	0.0	0.0	0.008167799268165186	0.0
8	0.0	0.0	0.0	0.008984579194981704	0.0
9	0.0	0.0	0.0	0.012251698902247778	0.0
10	0.0	0.0	0.0	0.01715237846314689	0.0
11	0.0	0.0	0.0	0.01715237846314689	0.0
12	0.0	0.0	0.0	0.020419498170412963	0.0
13	0.0	0.0	0.0	0.022053058024046	0.0
14	0.0	0.0	0.0	0.022053058024046	0.0
15	0.0	0.0	0.0	0.02286983795086252	0.0
16	0.0	0.0	0.0	0.02286983795086252	0.0
17	0.0	0.0	0.0	0.023686617877679037	0.0
18	0.0	0.0	0.0	0.029404077365394667	0.0
19	0.0	0.0	0.0	0.03348797699947726	0.0
20	0.0	0.0	0.0	0.03430475692629378	0.0
21	0.0	0.0	0.0	0.03920543648719289	0.0
22	0.0	0.0	0.0	0.040838996340825925	0.0
23	0.0	0.0	0.0	0.044106116048092	0.0
24	0.0	0.0	0.0	0.04573967590172504	0.0
25	0.0	0.0	0.0	0.047373235755358074	0.0
26	0.0	0.0	0.0	0.05145713538944067	0.0
27	0.0	0.0	0.0	0.07677731312075274	0.0
28	0.0	0.0	0.0	0.12006664924202823	0.0
29	0.0	0.0	0.0	0.1674398849973863	0.0
30	0.0	0.0	0.0	0.21726346053319393	0.0
31	0.0	0.0	0.0	0.2687205959226346	0.0
32	0.0	0.0	0.0	0.3552992681651856	0.0
33	0.0	0.0	0.0	0.4622974385781495	0.0
34	0.0	0.0	0.0	0.6477064819654992	0.0
35	0.0	0.0	0.0	0.8715041819132253	0.0
36	0.0	0.0	0.0	1.2031168322007317	0.0
37	0.0	0.0	0.0	1.6670478306325143	0.0
38	0.0	0.0	0.0	2.2298092002090955	0.0
39	0.0	0.0	0.0	2.8023719289074753	0.0
40	0.0	0.0	0.0	3.379835337166754	0.0
41	0.0	0.0	0.0	4.001404861474124	0.0
42	0.0	0.0	0.0	4.737323575535807	0.0
43	0.0	0.0	0.0	5.497745687401986	0.0
44	0.0	0.0	0.0	6.229580501829587	0.0
45	0.0	0.0	0.0	6.95161395713539	0.0
46	0.0	0.0	0.0	7.692433350757971	0.0
47	0.0	0.0	0.0	8.410382906429692	0.0
48	0.0	0.0	0.0	9.107096184004181	0.0
49	0.0	0.0	0.0	9.80871014113957	0.0
50	0.0	0.0	0.0	10.435997124934657	0.0
51	0.0	0.0	0.0	11.064917668583377	0.0
52	0.0	0.0	0.0	11.724875849451124	0.0
53	0.0	0.0	0.0	12.421589127025614	0.0
54	0.0	0.0	0.0	13.083180867746995	0.0
55	0.0	0.0	0.0	13.736604809200209	0.0
56	0.0	0.0	0.0	14.359807893361213	0.0
57	0.0	0.0	0.0	14.908684004181913	0.0
58	0.0	0.0	0.0	15.422438578149503	0.0
59	0.0	0.0	0.0	15.949261630946157	0.0
60	0.0	0.0	0.0	16.49568740198641	0.0
61	0.0	0.0	0.0	17.0143426555149	0.0
62	0.0	0.0	0.0	17.55995164662833	0.0
63	0.0	0.0	0.0	18.023882645060116	0.0
64	0.0	0.0	0.0	18.600529273392578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATACT	15	0.0022201454	70.0	8
TGCGCCA	15	0.0022201454	70.0	56
CGATGGG	15	0.0022201454	70.0	58
TTGCCGG	15	0.0022201454	70.0	13
TCTTTAT	85	0.0	65.882355	1
CTTTATA	95	0.0	58.947365	2
ATCGTAT	30	8.335632E-6	58.333332	39
CTATACA	70	0.0	55.0	1
TATATCG	20	0.0069372808	52.5	17
TCTAGCA	20	0.0069372808	52.5	3
GACCATA	20	0.0069372808	52.5	57
ACGATGT	20	0.0069372808	52.5	22
ACCTATG	20	0.0069372808	52.5	63
TACGATG	20	0.0069372808	52.5	21
CACCGTG	20	0.0069372808	52.5	21
GATTATC	20	0.0069372808	52.5	66
ATTTGGC	20	0.0069372808	52.5	8
GCACTGG	20	0.0069372808	52.5	1
GTACGAT	20	0.0069372808	52.5	20
TCTCGTA	2530	0.0	49.110672	38
>>END_MODULE