FastQCFastQC Report
Wed 25 May 2016
SRR1779751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82992
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA150.002217614370.028
TTAGTAA150.002217614370.08
TCGCGTA207.885399E-570.034
TGCGTGC150.002217614370.017
ATTTCGG207.885399E-570.042
CGGTGGT1500.060.66666846
CTCGGTG1850.060.54054344
TCTCGGT1850.060.54054343
TCGGTGG1650.059.3939445
AAGTGTA308.312036E-658.33333234
GACTAGC308.312036E-658.33333223
ATCTCGG1750.058.042
TTTCGGT252.3780817E-456.00000443
GTAGAGT252.3780817E-456.00000438
CGTGTAT252.3780817E-456.00000435
CTAGCTA252.3780817E-456.00000425
TAGATCT1650.055.15151639
CTTCCGA700.055.018
CGTATCA900.054.44444756
CGGTGTT452.6628186E-854.44444746
Produced by FastQC (version 0.11.5)