FastQCFastQC Report
Wed 25 May 2016
SRR1779751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82992
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2235626.937536148062463No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG24842.9930595720069406No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC14571.7555909003277426No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT8431.0157605552342395Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC7270.8759880470406786No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC5870.7072970888760363No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT4690.565114709851552No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3710.44703103913630227No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2920.3518411413148255No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2240.2699055330634278No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT1860.22411798727588203Illumina PCR Primer Index 9 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1790.2156834393676499No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1650.19881434355118566No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1580.19037979564295354No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG1470.1771255060728745No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1270.15302679776363987No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT930.11205899363794102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC401.3278623E-1070.02
TTCCGCG150.002217614370.016
TCCTACC150.002217614370.042
TACCTCA150.002217614370.062
TCTTTAT950.062.6315841
TACTGGG252.3780817E-456.0000045
TCGTCAA252.3780817E-456.00000432
GGGGGAC252.3780817E-456.00000410
TCTGCGA200.006929433452.55
TTAATAC200.006929433452.52
TCTTCCG200.006929433452.514
CCGGAAG200.006929433452.538
TTCGTCA200.006929433452.531
ATCGTAT200.006929433452.539
AGTTCTT200.006929433452.511
CAGTCCC200.006929433452.547
TCCTTAT200.006929433452.51
TTCTGCG200.006929433452.54
ACTGGGA200.006929433452.56
CGGACCT200.006929433452.539
Produced by FastQC (version 0.11.5)