FastQCFastQC Report
Wed 25 May 2016
SRR1779750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112411
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG150.002220063269.9968835
GTAGATC1500.062.997238
TAGATCT2050.061.46068239
AGATCTC1950.059.22813440
TGTAGAT1950.057.43334237
ATCTCGG1800.056.38638342
GATCTCG1800.056.38638341
TGGTCGC1150.054.7801749
TCGGTGG1550.054.1911445
CTCGGTG1700.053.5270344
TCTCGGT1700.053.5270343
CCGGATA200.006937032652.4976654
CATAGTT200.006937032652.49766521
ACTCGGT200.006937032652.49766543
CGTGTCA200.006937032652.49766556
CGACTAT200.006937032652.49766522
CTAATTC200.006937032652.49766527
CGCCGTA1150.051.73682853
GTGTCGA552.2555469E-950.9068336
ATCGTGT352.0759082E-549.99777633
Produced by FastQC (version 0.11.5)