FastQCFastQC Report
Wed 25 May 2016
SRR1779750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112411
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2370321.08601471386252No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG26482.3556413518249992No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC16071.4295753974255188No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT8150.7250180142512743RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7470.6645257136757079No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC5960.5301972226917294RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT5270.46881532946064No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3750.3335972458211385No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2490.22150857122523598No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2210.19659997687059097No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2090.18592486500431452No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1890.1681330118938538RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT1590.14144523222816272No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT1590.14144523222816272No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG1560.13877645426159363No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT1500.1334388983284554RNA PCR Primer, Index 42 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1330.11831582318456378No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT1190.10586152600724127No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAAGC150.002219671870.031
ATAGATA150.002219671870.061
TCGATAT150.002219671870.034
ATATCGT150.002219671870.037
ACAATTG150.002219671870.042
CTCGATA150.002219671870.033
TCTTTAT1200.061.251
CTTTATA1300.056.5384642
ACCCGAT252.3817352E-456.00000432
CTATACA950.055.2631571
TCTTAAT452.6742782E-854.4444471
ATCTCGT31200.053.17307737
TCTCGTA31300.053.11501738
CGATCTC31050.052.64090335
TCGATCT30700.052.55700734
GATCTCG31500.052.55555736
TCATTGA200.006935811652.528
TGAACTA200.006935811652.532
GTAGGTA200.006935811652.513
TTTCACA200.006935811652.521
Produced by FastQC (version 0.11.5)