##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779750_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	112411
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.67541432777931	34.0	34.0	34.0	34.0	34.0
2	33.67248756794264	34.0	34.0	34.0	34.0	34.0
3	33.68664098709201	34.0	34.0	34.0	34.0	34.0
4	33.72793587816139	34.0	34.0	34.0	34.0	34.0
5	33.734652302710586	34.0	34.0	34.0	34.0	34.0
6	37.403412477426585	38.0	38.0	38.0	37.0	38.0
7	37.3963757995214	38.0	38.0	38.0	37.0	38.0
8	37.299552534894275	38.0	38.0	38.0	36.0	38.0
9	37.29301402887618	38.0	38.0	38.0	36.0	38.0
10-11	37.21287062654011	38.0	38.0	38.0	36.0	38.0
12-13	37.18725480602432	38.0	38.0	38.0	36.0	38.0
14-15	37.218652978801	38.0	38.0	38.0	36.0	38.0
16-17	37.15619912642001	38.0	38.0	38.0	36.0	38.0
18-19	37.13348337796123	38.0	38.0	38.0	36.0	38.0
20-21	37.16038465986425	38.0	38.0	38.0	36.0	38.0
22-23	37.149936394125135	38.0	38.0	38.0	36.0	38.0
24-25	37.1049363496455	38.0	38.0	38.0	35.5	38.0
26-27	37.01144905747658	38.0	37.5	38.0	35.0	38.0
28-29	36.899573885118	38.0	37.0	38.0	35.0	38.0
30-31	36.742987785892836	38.0	37.0	38.0	34.5	38.0
32-33	36.68091201039044	38.0	37.0	38.0	34.0	38.0
34-35	36.52761740399072	38.0	37.0	38.0	34.0	38.0
36-37	36.72504914999422	38.0	37.0	38.0	34.0	38.0
38-39	35.4710882386955	38.0	36.0	38.0	31.0	38.0
40-41	36.47188887208547	38.0	37.0	38.0	34.0	38.0
42-43	36.92967325261763	38.0	37.0	38.0	35.0	38.0
44-45	36.9238553166505	38.0	38.0	38.0	35.0	38.0
46-47	36.96263710846803	38.0	38.0	38.0	35.5	38.0
48-49	36.94158489827508	38.0	38.0	38.0	35.5	38.0
50-51	36.83508731351914	38.0	37.5	38.0	34.0	38.0
52-53	36.81757568209517	38.0	37.5	38.0	35.0	38.0
54-55	36.81949275426782	38.0	37.5	38.0	35.0	38.0
56-57	36.83757372499133	38.0	37.0	38.0	35.0	38.0
58-59	36.72012970260918	38.0	37.0	38.0	34.5	38.0
60-61	36.751990463566734	38.0	37.0	38.0	34.5	38.0
62-63	36.795002268461275	38.0	37.0	38.0	35.0	38.0
64-65	36.830230137619985	38.0	38.0	38.0	35.0	38.0
66-67	36.733851669320615	38.0	37.5	38.0	34.0	38.0
68-69	36.45959470158614	38.0	37.0	38.0	34.0	38.0
70-71	34.524663956374376	38.0	34.0	38.0	27.5	38.0
72-73	31.23720098566866	38.0	23.5	38.0	11.0	38.0
74-75	30.831600110309488	38.0	23.0	38.0	11.0	38.0
76	30.485477399898585	37.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	2.0
21	4.0
22	8.0
23	18.0
24	27.0
25	76.0
26	119.0
27	261.0
28	405.0
29	767.0
30	1197.0
31	1815.0
32	2925.0
33	4505.0
34	8307.0
35	19028.0
36	19897.0
37	53046.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.556618124560764	12.625098967182927	11.239113609878038	45.579169298378275
2	16.251078631094824	13.028974032790385	47.654588963713515	23.065358372401278
3	16.170125699442224	15.099056142192492	44.952896068890055	23.77792208947523
4	12.780777681899458	33.88369465621692	27.514211242671983	25.821316419211644
5	13.041428329967708	26.30258604584961	46.59330492567453	14.062680698508153
6	24.668404337653786	46.43228865502486	14.086699700207275	14.812607307114073
7	22.67304801131562	22.682833530526374	15.526950209499072	39.11716824865894
8	19.95979041197036	46.94469402460613	13.133945966142106	19.961569597281407
9	18.362082002650986	14.95850050261985	13.08323918477729	53.59617830995187
10-11	21.290176228305057	29.383690208253643	27.74239175881364	21.58374180462766
12-13	20.538025638060333	14.726316819528337	30.255046214338456	34.480611328072875
14-15	19.606622127727714	14.215690635258117	16.74346816592682	49.43421907108735
16-17	33.176913291403864	27.703249681970625	14.594212310183167	24.52562471644234
18-19	34.10342404213111	18.469722713969272	15.001200950084955	32.42565229381466
20-21	20.43216411205309	15.964629796016405	15.191573778366884	48.411632313563615
22-23	20.48509487505671	29.050982555088023	14.92603037069326	35.537892199162
24-25	33.10352189732322	29.11325404097464	14.785919527448382	22.997304534253765
26-27	33.34015354369234	31.050786844703808	15.673732997660373	19.935326613943474
28-29	20.260917525864908	30.64646698276859	16.47036322068125	32.62225227068525
30-31	18.880714520820916	18.87715615019882	29.034969887288607	33.207159441691644
32-33	18.970563379028743	28.46740977306491	17.661527786426596	34.90049906147975
34-35	19.639537055982064	16.973872663707287	29.189314213021856	34.19727606728879
36-37	32.193913407050914	27.11789771463647	17.746928681356806	22.941260196955817
38-39	20.050084066505946	18.04894538790688	29.821814591098732	32.07915595448844
40-41	18.86203307505493	15.123075143891612	31.60633745807795	34.40855432297551
42-43	31.50447909902056	15.021661581161986	30.881764240154435	22.59209507966302
44-45	19.537233900596917	27.878054638780903	32.47902785314604	20.105683607476138
46-47	31.061461956570085	16.183024792947307	21.05354458193593	31.70196866854667
48-49	30.42451361521559	14.953162946686712	18.676108210050618	35.94621522804708
50-51	19.371324870341873	15.25740363487559	31.815836528453623	33.55543496632892
52-53	16.84799530295078	15.261406801825444	44.76163364795261	23.128964247271174
54-55	16.647836955458096	15.2191511506881	34.54999955520367	33.58301233865013
56-57	29.197320546921564	15.425536646769444	21.75810196511018	33.61904084119882
58-59	18.191725009118326	16.316018894948	42.380638905445196	23.111617190488477
60-61	29.38680378254797	29.041641832205034	20.908096182757916	20.66345820248908
62-63	17.075731022764675	45.45151275231072	16.37917997349014	21.093576251434467
64-65	16.183024792947307	47.10259672096147	15.682628924215603	21.031749561875618
66-67	16.3854071220788	47.229808470701265	15.243170152387222	21.14161425483271
68-69	15.940165997989519	47.61633647952602	15.270747524708437	21.17274999777602
70-71	16.30890215370382	44.991593349405306	16.020674133314355	22.67883036357652
72-73	16.87468308261647	38.220903648219476	18.39588652356086	26.508526745603188
74-75	17.16958304792236	35.07130085134017	19.73694745176184	28.022168648975633
76	17.32303778100008	35.36753520562934	19.36732170339202	27.942105309978558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	1.5
17	2.0
18	3.5
19	13.0
20	21.0
21	21.0
22	27.0
23	44.5
24	79.5
25	103.0
26	124.5
27	201.5
28	257.5
29	258.0
30	322.5
31	426.0
32	625.5
33	786.0
34	894.5
35	1129.0
36	1372.0
37	1489.0
38	1691.5
39	2315.0
40	7622.5
41	13073.5
42	13638.0
43	12146.0
44	9021.5
45	6248.0
46	5107.0
47	4495.5
48	3522.5
49	3038.5
50	2916.0
51	2813.5
52	2368.5
53	1972.5
54	1919.0
55	1874.5
56	1836.5
57	1838.0
58	1833.0
59	1781.5
60	1802.5
61	1968.0
62	2061.0
63	2018.0
64	2002.5
65	2007.5
66	1958.5
67	1932.0
68	2025.5
69	2068.0
70	1893.0
71	1769.0
72	1787.0
73	1739.0
74	1625.0
75	1577.0
76	1547.5
77	1357.0
78	1001.5
79	807.0
80	772.5
81	609.5
82	405.5
83	330.0
84	269.5
85	160.0
86	93.0
87	75.0
88	55.0
89	24.5
90	9.0
91	4.5
92	5.0
93	2.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	112411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.844552579373904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.47299250887107	16.743912962254583
2	25.141280063083194	17.01790750015568
3	12.95571034301485	13.154406597219134
4	5.929819950059141	8.027684123439876
5	2.6968064134577476	4.563610322833175
6	1.2932054146405572	2.6260775191040024
7	0.7727690892364305	1.8307816850664083
8	0.48889472992508876	1.3237138714182777
9	0.2917597581811013	0.888703062867513
>10	0.8963070048626627	4.360783197373923
>50	0.013142331449599158	0.31758457802172385
>100	0.02891312918911815	1.8850468370533133
>500	0.010513865159679326	2.3885562800793516
>1k	0.005256932579839663	3.785216749250518
>5k	0.0	0.0
>10k+	0.0026284662899198315	21.08601471386252
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	23703	21.08601471386252	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	2648	2.3556413518249992	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	1607	1.4295753974255188	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	815	0.7250180142512743	RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	747	0.6645257136757079	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	596	0.5301972226917294	RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	527	0.46881532946064	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	375	0.3335972458211385	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	249	0.22150857122523598	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	221	0.19659997687059097	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	209	0.18592486500431452	No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	189	0.1681330118938538	RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT	159	0.14144523222816272	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	159	0.14144523222816272	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG	156	0.13877645426159363	No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT	150	0.1334388983284554	RNA PCR Primer, Index 42 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT	133	0.11831582318456378	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT	119	0.10586152600724127	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001779185311046072	0.0
2	0.0	0.0	0.0	0.001779185311046072	0.0
3	0.0	0.0	0.0	0.003558370622092144	0.0
4	0.0	0.0	0.0	0.006227148588661252	0.0
5	0.0	0.0	0.0	0.012454297177322503	0.0
6	0.0	0.0	0.0	0.012454297177322503	0.0
7	0.0	0.0	0.0	0.012454297177322503	0.0
8	0.0	0.0	0.0	0.01334388983284554	0.0
9	0.0	0.0	0.0	0.01779185311046072	0.0
10	0.0	0.0	0.0	0.023129409043598937	0.0
11	0.0	0.0	0.0	0.02846696497673715	0.0
12	0.0	0.0	0.0	0.029356557632260187	0.0
13	0.0	0.0	0.0	0.032914928254352334	0.0
14	0.0	0.0	0.0	0.03469411356539841	0.0
15	0.0	0.0	0.0	0.040921262154059654	0.0
16	0.0	0.0	0.0	0.040921262154059654	0.0
17	0.0	0.0	0.0	0.040921262154059654	0.0
18	0.0	0.0	0.0	0.04270044746510573	0.0
19	0.0	0.0	0.0	0.046258818087197874	0.0
20	0.0	0.0	0.0	0.04892759605376698	0.0
21	0.0	0.0	0.0	0.05159637402033609	0.0
22	0.0	0.0	0.0	0.05782352260899734	0.0
23	0.0	0.0	0.0	0.06138189323108948	0.0
24	0.0	0.0	0.0	0.06227148588661252	0.0
25	0.0	0.0	0.0	0.07116741244184288	0.0
26	0.0	0.0	0.0	0.08184252430811931	0.0
27	0.0	0.0	0.0	0.15656830737205435	0.0
28	0.0	0.0	0.0	0.21350223732552864	0.0
29	0.0	0.0	0.0	0.2802216864897563	0.0
30	0.0	0.0	0.0	0.36028502548682956	0.0
31	0.0	0.0	0.0	0.43412121589524155	0.0
32	0.0	0.0	0.0	0.5230804814475452	0.0
33	0.0	0.0	0.0	0.6565193797760006	0.0
34	0.0	0.0	0.0	0.8486713933689763	0.0
35	0.0	0.0	0.0	1.1315618578253017	0.0
36	0.0	0.0	0.0	1.5452224426435135	0.0
37	0.0	0.0	0.0	2.1118929642116875	0.0
38	0.0	0.0	0.0	2.708809636067645	0.0
39	0.0	0.0	0.0	3.2754801576358186	0.0
40	0.0	0.0	0.0	3.8910782752577595	0.0
41	0.0	0.0	0.0	4.546708062378237	0.0
42	0.0	0.0	0.0	5.231694407130974	0.0
43	0.0	0.0	0.0	5.93358301233865	0.0
44	0.0	0.0	0.0	6.667496953145155	0.0
45	0.0	0.0	0.0	7.346256149309231	0.0
46	0.0	0.0	0.0	8.15489587317967	0.0
47	0.0	0.0	0.0	8.873686738842284	0.0
48	0.0	0.0	0.0	9.526647747996192	0.0
49	0.0	0.0	0.0	10.139577087651565	0.0
50	0.0	0.0	0.0	10.760512761206643	0.0
51	0.0	0.0	0.0	11.350312691818417	0.0
52	0.0	0.0	0.0	11.962352438818264	0.0
53	0.0	0.0	0.0	12.536139701630624	0.0
54	0.0	0.0	0.0	13.175756820951687	0.0
55	0.0	0.0	0.0	13.73086263799806	0.0
56	0.0	0.0	0.0	14.300201937532803	0.0
57	0.0	0.0	0.0	14.828619974913487	0.0
58	0.0	0.0	0.0	15.324123084039819	0.0
59	0.0	0.0	0.0	15.832970082998994	0.0
60	0.0	0.0	0.0	16.246630667817207	0.0
61	0.0	0.0	0.0	16.737685813665923	0.0
62	0.0	0.0	0.0	17.258097517146897	0.0
63	0.0	0.0	0.0	17.74114632909591	0.0
64	0.0	0.0	0.0	18.240207808844332	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAAGC	15	0.0022196718	70.0	31
ATAGATA	15	0.0022196718	70.0	61
TCGATAT	15	0.0022196718	70.0	34
ATATCGT	15	0.0022196718	70.0	37
ACAATTG	15	0.0022196718	70.0	42
CTCGATA	15	0.0022196718	70.0	33
TCTTTAT	120	0.0	61.25	1
CTTTATA	130	0.0	56.538464	2
ACCCGAT	25	2.3817352E-4	56.000004	32
CTATACA	95	0.0	55.263157	1
TCTTAAT	45	2.6742782E-8	54.444447	1
ATCTCGT	3120	0.0	53.173077	37
TCTCGTA	3130	0.0	53.115017	38
CGATCTC	3105	0.0	52.640903	35
TCGATCT	3070	0.0	52.557007	34
GATCTCG	3150	0.0	52.555557	36
TCATTGA	20	0.0069358116	52.5	28
TGAACTA	20	0.0069358116	52.5	32
GTAGGTA	20	0.0069358116	52.5	13
TTTCACA	20	0.0069358116	52.5	21
>>END_MODULE