FastQCFastQC Report
Wed 25 May 2016
SRR1779749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146890
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA150.00222128769.9979641
CCCGTGT850.061.76290533
CAGTGTA401.0588337E-861.2482134
ACGTGTA308.345331E-658.33163534
GCCGTAT800.056.87333754
AGAACTC200.00694082552.49846640
GCGCTCA200.00694082552.49846611
CCGACTA750.051.33183321
CCGTGTA900.050.5540834
GTAGATC1600.050.3110338
CAAGTGT305.8634114E-446.66530633
CTAAAAT305.8634114E-446.66530625
GCGTAGA305.8634114E-446.66530636
CGTATCA1050.046.66530256
AGTGTAG1500.046.66530235
ACGCTTC1400.044.99868415
TTCGTGT551.3024692E-744.5441533
GTGTCGC551.3024692E-744.5441536
TAGATCT2050.044.38894739
ATCTCGG1600.043.74872242
Produced by FastQC (version 0.11.5)