##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779749_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146890
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.774184764109197	33.0	23.0	34.0	12.0	34.0
2	26.81878957042685	32.0	21.0	34.0	12.0	34.0
3	25.934297773844374	31.0	21.0	34.0	11.0	34.0
4	27.617074000953092	32.0	23.0	34.0	12.0	34.0
5	27.42018517257812	33.0	23.0	34.0	11.0	34.0
6	29.32320784260331	34.0	21.0	38.0	11.0	38.0
7	30.397957655388385	36.0	26.0	38.0	12.0	38.0
8	29.582919191231532	35.0	22.0	38.0	11.0	38.0
9	31.0168289195997	37.0	26.0	38.0	12.0	38.0
10-11	30.572921914357682	37.0	26.0	38.0	11.0	38.0
12-13	29.96319354619103	35.5	24.0	38.0	11.0	38.0
14-15	29.62592756484444	35.0	23.0	38.0	11.0	38.0
16-17	29.424079242970933	35.5	23.5	38.0	11.0	38.0
18-19	29.97369460140241	35.5	24.0	38.0	11.0	38.0
20-21	28.949002655047995	34.0	22.5	37.5	11.0	38.0
22-23	28.981043638096537	33.5	23.5	37.5	11.0	38.0
24-25	29.831482742188033	35.0	24.0	38.0	11.0	38.0
26-27	28.60367962420859	34.0	21.5	38.0	11.0	38.0
28-29	30.31624004357002	36.5	24.0	38.0	11.0	38.0
30-31	30.749060521478658	37.0	25.5	38.0	11.0	38.0
32-33	30.855078630267545	37.0	26.0	38.0	11.0	38.0
34-35	30.92730614745728	37.0	26.0	38.0	11.0	38.0
36-37	29.23422969569065	35.0	22.5	38.0	11.0	38.0
38-39	27.95812512764654	33.5	21.0	37.0	11.0	38.0
40-41	28.63911089931241	34.0	21.5	37.5	11.0	38.0
42-43	29.516498740554155	35.0	23.5	38.0	11.0	38.0
44-45	30.90708012798693	37.0	26.0	38.0	11.0	38.0
46-47	29.753873646946694	35.5	23.5	38.0	11.0	38.0
48-49	29.171461637960377	35.0	22.5	38.0	11.0	38.0
50-51	27.38892368438968	31.5	21.0	37.0	11.0	38.0
52-53	30.07542378650691	35.5	24.0	38.0	11.0	38.0
54-55	29.33943767445027	35.0	22.5	38.0	11.0	38.0
56-57	29.732874940431614	35.5	23.0	38.0	11.0	38.0
58-59	30.627986928994485	37.0	25.0	38.0	11.0	38.0
60-61	31.095605555177343	37.0	26.0	38.0	11.0	38.0
62-63	31.008805909183742	37.0	25.5	38.0	11.0	38.0
64-65	30.754111920484718	37.0	25.0	38.0	11.0	38.0
66-67	29.071379944175916	34.5	21.5	38.0	11.0	38.0
68-69	29.081561032064812	34.5	22.0	38.0	11.0	38.0
70-71	28.769337599564302	34.0	21.5	38.0	11.0	38.0
72-73	27.779375723330382	32.5	21.0	37.0	11.0	38.0
74-75	29.30604193614269	35.0	22.5	37.5	11.0	38.0
76	29.59464905711757	36.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	0.0
5	0.0
6	0.0
7	2.0
8	1.0
9	0.0
10	0.0
11	15.0
12	83.0
13	427.0
14	1153.0
15	2010.0
16	3303.0
17	5080.0
18	6795.0
19	6817.0
20	5968.0
21	4520.0
22	3503.0
23	2740.0
24	2482.0
25	2432.0
26	2416.0
27	2717.0
28	3098.0
29	3636.0
30	4481.0
31	5633.0
32	7096.0
33	9423.0
34	13208.0
35	17205.0
36	19387.0
37	11256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.80767921573967	18.17074000953094	14.185444890734564	32.83613588399483
2	18.169378446456534	16.305398597590035	42.39090475866295	23.13431819729049
3	18.507046088910066	17.366056232554975	39.46422493021989	24.662672748315064
4	14.28289388585939	20.232965028014352	31.206557332407463	34.277583753718794
5	14.06630812172374	27.5580366260467	40.37851453468582	17.99714071754374
6	26.189665736265233	33.10504459119068	17.400776090952412	23.304513581591667
7	24.74436653277963	24.560555517734358	20.513309279052354	30.181768670433655
8	21.57606290635531	32.61190727439834	18.71940633829186	27.092623480954487
9	21.483619727131614	14.830069987200783	20.239781051714278	43.44652923395332
10-11	24.019798070573177	22.90836924627084	27.548797342102215	25.523035341053763
12-13	22.877917268048257	18.19190653849296	30.048882111053622	28.881294082405162
14-15	24.249077515896683	22.115790476968535	23.05155018177362	30.58358182536117
16-17	26.42660430219535	20.59729640485163	24.907661774868345	28.06843751808468
18-19	21.76608151302554	20.51373698382062	28.04005868556587	29.680122817587968
20-21	25.959764441569938	20.395207134833374	25.788201654355447	27.856826769241245
22-23	27.127125423382637	19.191871053393015	25.55954589552874	28.121457627695605
24-25	22.191510365251727	23.704939238179527	22.925758246247064	31.177792150321682
26-27	25.03940117029135	22.836680021649368	25.547616697245836	26.576302110813447
28-29	23.13018259058045	23.348719415056575	24.968342796454394	28.55275519790858
30-31	22.60967838566488	23.051523651316685	25.803014623784755	28.535783339233685
32-33	22.98483177430116	22.72068134471631	25.75160328417957	28.542883596802966
34-35	23.444792031888785	22.510731147730713	25.49673044650425	28.547746373876247
36-37	25.56252255196313	19.670417951703058	29.684510801113813	25.08254869522
38-39	25.40431977941552	18.070907017514	33.72219290929825	22.80258029377223
40-41	23.72909050060252	22.69187040025054	25.040338228385856	28.53870087076108
42-43	20.237803466633988	23.250343804038508	26.997807823754478	29.514044905573027
44-45	20.459953228557133	20.48922793078916	29.808454942488826	29.242363898164886
46-47	24.608707492255846	19.624536201790516	28.479763079960513	27.286993225993122
48-49	24.154681463074226	20.184501216959134	29.844603679812092	25.816213640154544
50-51	28.49619939476255	18.513525933641738	29.288658776113202	23.701615895482504
52-53	19.222913631350337	22.14022140221402	29.57680314810528	29.06006181833036
54-55	23.283917416982963	20.759450581246917	27.546508263407826	28.410123738362298
56-57	23.463514097715567	21.200186539855874	26.623118006882912	28.713181355545647
58-59	19.770497606938857	22.906326804327254	28.296331093455336	29.026844495278553
60-61	19.72113859330215	21.51065814287562	28.000857825616304	30.76734543820592
62-63	19.475497337999208	22.961629062785093	27.626938631009928	29.935934968205768
64-65	20.05221807684976	23.678889463794558	26.86337518041448	29.405517278941208
66-67	18.53792211328976	27.023760893246184	22.139161220043572	32.29915577342048
68-69	18.942006829935956	26.888702005740416	22.636164613032715	31.533126551290913
70-71	18.62304903579392	27.744966214491175	22.062192568890097	31.569792180824805
72-73	17.727193815548954	28.729716205590105	21.36134173449207	32.181748244368876
74-75	19.28787827211764	26.13881608060728	25.49205160499711	29.08125404227797
76	20.29846884935629	24.90621787409026	27.13656447648809	27.658748800065357
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.0
4	2.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	2.0
12	1.0
13	0.5
14	0.5
15	1.5
16	5.0
17	8.0
18	9.5
19	18.5
20	34.0
21	42.0
22	59.5
23	90.0
24	140.5
25	178.0
26	224.5
27	361.5
28	564.5
29	677.0
30	777.0
31	1051.5
32	1442.0
33	1658.0
34	1932.0
35	2544.0
36	3237.5
37	3593.0
38	3971.5
39	4809.0
40	5631.0
41	6368.5
42	6743.0
43	7087.5
44	7620.5
45	7821.0
46	7833.0
47	7826.5
48	7599.0
49	7152.0
50	6926.0
51	6593.5
52	5833.5
53	5256.0
54	5106.0
55	4895.5
56	4379.0
57	3952.0
58	3831.0
59	3678.0
60	3375.0
61	3153.5
62	3082.0
63	2985.5
64	2802.0
65	2629.0
66	2455.0
67	2367.0
68	2324.5
69	2213.5
70	2063.5
71	1982.0
72	1952.5
73	1784.5
74	1518.0
75	1390.0
76	1250.5
77	1005.0
78	785.0
79	671.0
80	579.0
81	398.5
82	272.0
83	234.0
84	177.0
85	94.0
86	45.0
87	22.0
88	20.5
89	13.0
90	7.0
91	4.5
92	2.0
93	2.5
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	6.80781537204711E-4
5	0.0
6	0.0
7	0.0
8	0.0034039076860235555
9	0.004084689223228266
10-11	0.004765470760432977
12-13	0.004084689223228266
14-15	0.002723126148818844
16-17	0.007828987677854176
18-19	0.0037442984546259108
20-21	0.0034039076860235555
22-23	0.005105861529035333
24-25	0.0034039076860235555
26-27	0.0023827353802164885
28-29	0.002723126148818844
30-31	0.004084689223228266
32-33	0.002723126148818844
34-35	0.0030635169174211994
36-37	0.004765470760432977
38-39	0.005105861529035333
40-41	0.004765470760432977
42-43	0.002723126148818844
44-45	0.0037442984546259108
46-47	0.0034039076860235555
48-49	0.005105861529035333
50-51	0.0037442984546259108
52-53	0.005446252297637688
54-55	0.005105861529035333
56-57	0.0030635169174211994
58-59	0.004765470760432977
60-61	0.004765470760432977
62-63	0.005446252297637688
64-65	0.004084689223228266
66-67	0.006807815372047111
68-69	0.024848526107971954
70-71	0.005105861529035333
72-73	0.0037442984546259108
74-75	0.0034039076860235555
76	0.004765470760432977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	146890.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.37939073718353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.84471385628585	90.4576569913595
2	3.101116264068793	5.853629272159668
3	0.6814543182295324	1.9294573010917815
4	0.1960085186697223	0.7399665828538502
5	0.08095467805335733	0.38202265960003545
6	0.04689341890451214	0.2655463383474828
7	0.02094163223284757	0.13835209438248225
8	0.01794720669867225	0.13550771469239886
9	0.0039880837917119615	0.033875362662355045
>10	0.005982023065002283	0.06398568285044835
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.80781537204711E-4	0.0
4	0.0	0.0	0.0	6.80781537204711E-4	0.0
5	0.0	0.0	0.0	6.80781537204711E-4	0.0
6	0.0	0.0	0.0	0.001361563074409422	0.0
7	0.0	0.0	0.0	0.001361563074409422	0.0
8	0.0	0.0	0.0	0.001361563074409422	0.0
9	0.0	0.0	0.0	0.003403907686023555	0.0
10	0.0	0.0	0.0	0.010892504595275377	0.0
11	0.0	0.0	0.0	0.010892504595275377	0.0
12	0.0	0.0	6.80781537204711E-4	0.010892504595275377	0.0
13	0.0	0.0	6.80781537204711E-4	0.012934849206889509	0.0
14	0.0	0.0	6.80781537204711E-4	0.017700319967322485	0.0
15	0.0	0.0	6.80781537204711E-4	0.019061883041731908	0.0
16	0.0	0.0	6.80781537204711E-4	0.019061883041731908	0.0
17	0.0	0.0	6.80781537204711E-4	0.02110422765334604	0.0
18	0.0	0.0	6.80781537204711E-4	0.022465790727755463	0.0
19	0.0	0.0	6.80781537204711E-4	0.03267751378582613	0.0
20	0.0	0.0	6.80781537204711E-4	0.03540063993464497	0.0
21	0.0	0.0	6.80781537204711E-4	0.038804547620668525	0.0
22	0.0	0.0	6.80781537204711E-4	0.059908775274014565	0.0
23	0.0	0.0	6.80781537204711E-4	0.0619511198856287	0.0
24	0.0	0.0	6.80781537204711E-4	0.06943971679488052	0.0
25	0.0	0.0	6.80781537204711E-4	0.07352440601810879	0.0
26	0.0	0.0	6.80781537204711E-4	0.07828987677854177	0.0
27	0.0	0.0	6.80781537204711E-4	0.10960582748995847	0.0
28	0.0	0.0	6.80781537204711E-4	0.14568724896180815	0.0
29	0.0	0.0	6.80781537204711E-4	0.16883382122676832	0.0
30	0.0	0.0	6.80781537204711E-4	0.2042344611614133	0.0
31	0.0	0.0	6.80781537204711E-4	0.21785009190550753	0.0
32	0.0	0.0	6.80781537204711E-4	0.26958948873306554	0.0
33	0.0	0.0	6.80781537204711E-4	0.3281367009326707	0.0
34	0.0	0.0	6.80781537204711E-4	0.4043842330995983	0.0
35	0.0	0.0	6.80781537204711E-4	0.55347538974743	0.0
36	0.0	0.0	6.80781537204711E-4	0.6978010756348287	0.0
37	0.0	0.0	6.80781537204711E-4	1.0606576349649397	0.0
38	0.0	0.0	6.80781537204711E-4	1.4493838927088298	0.0
39	0.0	0.0	6.80781537204711E-4	1.8449179658247667	0.0
40	0.0	0.0	6.80781537204711E-4	2.3166995711076317	0.0
41	0.0	0.0	6.80781537204711E-4	2.819116345564708	0.0
42	0.0	0.0	6.80781537204711E-4	3.3092790523521	0.0
43	0.0	0.0	6.80781537204711E-4	3.8811355436040573	0.0
44	0.0	0.0	6.80781537204711E-4	4.407379671863299	0.0
45	0.0	0.0	6.80781537204711E-4	4.823337191095377	0.0
46	0.0	0.0	6.80781537204711E-4	5.286268636394581	0.0
47	0.0	0.0	6.80781537204711E-4	5.845190278439649	0.0
48	0.0	0.0	6.80781537204711E-4	6.210769963918579	0.0
49	0.0	0.0	6.80781537204711E-4	6.566818707876642	0.0
50	0.0	0.0	6.80781537204711E-4	7.0535775069780104	0.0
51	0.0	0.0	6.80781537204711E-4	7.630880250527606	0.0
52	0.0	0.0	6.80781537204711E-4	8.109469671182518	6.80781537204711E-4
53	0.0	0.0	6.80781537204711E-4	8.613928790251208	6.80781537204711E-4
54	0.0	0.0	6.80781537204711E-4	9.170808087684662	6.80781537204711E-4
55	0.0	0.0	6.80781537204711E-4	9.502348696303356	6.80781537204711E-4
56	0.0	0.0	6.80781537204711E-4	9.865205255633468	6.80781537204711E-4
57	0.0	0.0	6.80781537204711E-4	10.172918510449996	6.80781537204711E-4
58	0.0	0.0	6.80781537204711E-4	10.483354891415345	6.80781537204711E-4
59	0.0	0.0	6.80781537204711E-4	10.843488324596636	6.80781537204711E-4
60	0.0	0.0	6.80781537204711E-4	11.066784668799782	6.80781537204711E-4
61	0.0	0.0	6.80781537204711E-4	11.310504459119068	6.80781537204711E-4
62	0.0	0.0	6.80781537204711E-4	11.48410375110627	6.80781537204711E-4
63	0.0	0.0	6.80781537204711E-4	11.726461978351146	6.80781537204711E-4
64	0.0	0.0	6.80781537204711E-4	11.951119885628701	6.80781537204711E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCTA	15	0.002221287	69.99796	41
CCCGTGT	85	0.0	61.762905	33
CAGTGTA	40	1.0588337E-8	61.24821	34
ACGTGTA	30	8.345331E-6	58.331635	34
GCCGTAT	80	0.0	56.873337	54
AGAACTC	20	0.006940825	52.498466	40
GCGCTCA	20	0.006940825	52.498466	11
CCGACTA	75	0.0	51.331833	21
CCGTGTA	90	0.0	50.55408	34
GTAGATC	160	0.0	50.31103	38
CAAGTGT	30	5.8634114E-4	46.665306	33
CTAAAAT	30	5.8634114E-4	46.665306	25
GCGTAGA	30	5.8634114E-4	46.665306	36
CGTATCA	105	0.0	46.665302	56
AGTGTAG	150	0.0	46.665302	35
ACGCTTC	140	0.0	44.998684	15
TTCGTGT	55	1.3024692E-7	44.54415	33
GTGTCGC	55	1.3024692E-7	44.54415	36
TAGATCT	205	0.0	44.388947	39
ATCTCGG	160	0.0	43.748722	42
>>END_MODULE