FastQCFastQC Report
Wed 25 May 2016
SRR1779749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146890
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2838519.323983933555724No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG32632.221390155898972No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC19241.309823677581864No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT10260.6984818571720335RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC7220.49152426986180137No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC6860.4670161345224318No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC4380.2981823132956634No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT4090.27843964871672683No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2990.2035536796242086No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2860.19470351964054736No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC2520.17155694737558716No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2380.1620260058547212No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT2300.15657975355708353No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1850.12594458438287154No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT252.3841517E-456.00000439
CTATACA1250.056.01
TCTTTAT1150.054.7826081
TCGTTGT200.006940029652.534
TGCCCAT200.006940029652.512
GGTAAGC200.006940029652.516
GTCTACT200.006940029652.527
TATAACT200.006940029652.54
GCCAAGA200.006940029652.51
ATATTAG200.006940029652.558
TCTCGTA40500.050.72839438
AATCTCG40150.050.560436
ATCTCGT40650.050.54120637
TAATCTC40200.050.4975135
TATGCCG42300.050.47281643
CGTCTTC42100.050.4631848
TCGTATG42150.050.4033240
CTCGTAT41700.050.3597139
CGTATGC42300.050.3073341
CTGCTTG41900.050.28639654
Produced by FastQC (version 0.11.5)