##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779749_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146890
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.67737762951869	34.0	34.0	34.0	34.0	34.0
2	33.66855470079651	34.0	34.0	34.0	34.0	34.0
3	33.69293348764381	34.0	34.0	34.0	34.0	34.0
4	33.73080536455851	34.0	34.0	34.0	34.0	34.0
5	33.732241813602016	34.0	34.0	34.0	34.0	34.0
6	37.41017768398121	38.0	38.0	38.0	37.0	38.0
7	37.40291374497924	38.0	38.0	38.0	37.0	38.0
8	37.295418340254614	38.0	38.0	38.0	36.0	38.0
9	37.310375110627	38.0	38.0	38.0	36.0	38.0
10-11	37.22149908094492	38.0	38.0	38.0	36.0	38.0
12-13	37.19861460957179	38.0	38.0	38.0	36.0	38.0
14-15	37.216396623323575	38.0	38.0	38.0	36.0	38.0
16-17	37.166549799169445	38.0	38.0	38.0	36.0	38.0
18-19	37.14238545850637	38.0	38.0	38.0	36.0	38.0
20-21	37.193747021580776	38.0	38.0	38.0	36.0	38.0
22-23	37.15288310981006	38.0	38.0	38.0	36.0	38.0
24-25	37.132330315201855	38.0	38.0	38.0	36.0	38.0
26-27	37.01192388862414	38.0	38.0	38.0	35.5	38.0
28-29	36.953512832731974	38.0	38.0	38.0	35.5	38.0
30-31	36.821815644359724	38.0	37.5	38.0	34.5	38.0
32-33	36.72508339573831	38.0	37.0	38.0	34.0	38.0
34-35	36.708155762815714	38.0	37.0	38.0	34.0	38.0
36-37	36.72484852610798	38.0	37.0	38.0	34.5	38.0
38-39	35.979263394376744	38.0	37.0	38.0	33.0	38.0
40-41	36.664190891143036	38.0	37.0	38.0	34.5	38.0
42-43	36.946963714344065	38.0	37.5	38.0	35.0	38.0
44-45	36.96297229219144	38.0	38.0	38.0	35.0	38.0
46-47	36.9761079719518	38.0	38.0	38.0	35.5	38.0
48-49	36.94947920212404	38.0	38.0	38.0	35.5	38.0
50-51	36.85741711484785	38.0	37.5	38.0	35.0	38.0
52-53	36.86271699911498	38.0	38.0	38.0	35.0	38.0
54-55	36.85590918374294	38.0	38.0	38.0	35.0	38.0
56-57	36.85875825447614	38.0	38.0	38.0	35.0	38.0
58-59	36.76488528831098	38.0	37.0	38.0	34.5	38.0
60-61	36.80691333651031	38.0	37.0	38.0	35.0	38.0
62-63	36.85744094220165	38.0	37.5	38.0	35.0	38.0
64-65	36.88731363605419	38.0	38.0	38.0	35.0	38.0
66-67	36.78935938457349	38.0	38.0	38.0	34.5	38.0
68-69	36.54035332561781	38.0	37.0	38.0	34.0	38.0
70-71	34.75985771665872	38.0	35.0	38.0	27.5	38.0
72-73	31.8624446865001	38.0	25.5	38.0	11.0	38.0
74-75	31.49351215195044	38.0	25.0	38.0	11.0	38.0
76	31.1808087684662	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	7.0
22	17.0
23	22.0
24	48.0
25	98.0
26	153.0
27	310.0
28	533.0
29	859.0
30	1457.0
31	2246.0
32	3478.0
33	5481.0
34	9881.0
35	22646.0
36	26426.0
37	73225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.582748995847233	14.395125604193614	11.535843147933827	43.486282252025326
2	16.050105521138267	14.489754237865068	47.20403022670025	22.25611001429641
3	15.633467220368983	16.378923003608143	44.67969228674518	23.307917489277692
4	12.249302198924365	32.81230853019267	29.31649533664647	25.621893934236507
5	12.529784192252706	28.186397984886653	45.926884062904215	13.35693375995643
6	24.107835795493227	47.00183811015045	14.78180951732589	14.10851657703043
7	22.16488528831098	23.875008509769216	16.999114984001633	36.96099121791817
8	19.43903601334332	46.80236911974947	14.12417455238614	19.63442031452107
9	18.711280550071482	15.047995098372931	14.446184219483968	51.79454013207162
10-11	21.223024031588263	29.420995302607395	28.18469603104364	21.171284634760706
12-13	20.571516100483358	16.03002246579073	30.145346858193207	33.25311457553271
14-15	19.671182517530124	15.663081217237387	17.701341139628294	46.96439512560419
16-17	31.871468445775754	27.90183130233508	16.27714616379604	23.94955408809313
18-19	32.062427666961675	19.67867111443938	16.74109878140105	31.5178024371979
20-21	20.284226291782968	17.801075634828784	16.477295935734222	45.437402137654026
22-23	20.327796310164068	29.209272244536727	16.431002791204303	34.0319286540949
24-25	31.994689904009803	29.454693988699027	16.32514126216897	22.2254748451222
26-27	31.837429368915515	30.941520865954114	17.119272925318267	20.101776839812104
28-29	20.143644904350193	31.18013479474437	17.769759684117368	30.906460616788074
30-31	19.351555585812513	30.815576281571243	29.554428483899514	20.278439648716727
32-33	29.45809789638505	32.205732180543265	19.092858601674724	19.243311321396963
34-35	21.740758390632443	18.77323166995711	40.660358091088575	18.825651848321872
36-37	18.97678534958132	39.434610933351486	21.79590169514603	19.792702021921166
38-39	18.816120906801007	20.505480291374496	30.001361563074408	30.67703723875009
40-41	18.894070392810946	16.513717747974674	31.738716046020834	32.85349581319355
42-43	30.37340867315678	16.549458778677923	30.962965484376063	22.114167063789232
44-45	19.164340663081216	28.273878412417453	32.316699571107634	20.245081353393697
46-47	29.72019878820886	17.333378718769147	22.102253386888147	30.84416910613384
48-49	29.103070324732794	16.34522431751651	20.30192661175029	34.24977874600041
50-51	18.99142215263122	16.843556402750355	31.869766491932737	32.29525495268568
52-53	16.678466879978217	16.606304037034516	43.6837088978147	23.031520185172578
54-55	16.682551569201443	16.824494519708626	34.18782762611478	32.30512628497515
56-57	27.989992511403088	16.742119953706855	22.60671250595684	32.661175028933215
58-59	17.972972972972972	17.581863979848865	41.43304513581592	23.012117911362242
60-61	28.065218871264214	29.229695690652868	21.61243107086936	21.092654367213562
62-63	16.862618285792088	44.0176322418136	17.54169786915379	21.57805160324052
64-65	16.123289536387773	45.57628157124379	16.794199741303014	21.506229151065423
66-67	16.39015589897202	45.57253727278916	16.683232350738646	21.35407447750017
68-69	16.191367690108244	45.79345088161209	16.5749880863231	21.440193341956565
70-71	16.46810538498196	43.31506569541834	17.47532166927633	22.74150725032337
72-73	17.347675131050448	38.009054394444824	19.2055279460821	25.43774252842263
74-75	17.621008918238136	35.454762066852744	20.66342160800599	26.260807406903126
76	17.31499761726462	35.75736946014024	21.03887262577439	25.888760296820752
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.5
14	0.5
15	2.5
16	5.5
17	6.0
18	6.0
19	17.0
20	41.0
21	54.0
22	62.5
23	87.0
24	123.0
25	143.0
26	216.0
27	318.5
28	422.5
29	497.0
30	587.0
31	764.5
32	1076.0
33	1300.0
34	1449.0
35	2019.0
36	8242.0
37	14044.0
38	14815.5
39	14154.0
40	10958.0
41	7939.0
42	6683.0
43	6030.0
44	5522.5
45	5453.5
46	5239.0
47	5291.0
48	5004.0
49	4443.5
50	4222.0
51	4127.5
52	3661.5
53	3099.5
54	2909.0
55	2824.0
56	2659.0
57	2427.0
58	2275.0
59	2216.0
60	2165.0
61	2133.0
62	2093.0
63	2049.0
64	2040.5
65	2130.0
66	2117.5
67	2051.0
68	2134.5
69	2128.0
70	1994.5
71	1951.0
72	1899.0
73	1802.0
74	1690.5
75	1624.0
76	1519.5
77	1270.5
78	1035.0
79	944.0
80	865.0
81	651.0
82	436.0
83	356.0
84	294.0
85	203.5
86	129.0
87	83.0
88	71.0
89	35.5
90	13.5
91	8.5
92	2.0
93	3.0
94	3.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	146890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.460276397304103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.05513719380247	14.489073456327864
2	24.46767073487406	15.395193682347335
3	13.671773565307713	12.903533256178093
4	6.753657058772614	8.498876710463612
5	3.3000086557604087	5.1909592211859215
6	1.7138405608932743	3.2350738647967865
7	1.0213797282091233	2.249302198924365
8	0.7097723535012551	1.7863707536251616
9	0.5258374448195274	1.488869221866703
>10	1.7268242015061022	8.001906188304172
>50	0.01514758071496581	0.3240520117094424
>100	0.025967281225655673	1.924569405677718
>500	0.004327880204275946	0.9585404043842332
>1k	0.006491820306413918	4.2296956906528695
>5k	0.0	0.0
>10k+	0.002163940102137973	19.323983933555724
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	28385	19.323983933555724	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	3263	2.221390155898972	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	1924	1.309823677581864	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	1026	0.6984818571720335	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	722	0.49152426986180137	No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	686	0.4670161345224318	No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	438	0.2981823132956634	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT	409	0.27843964871672683	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	299	0.2035536796242086	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	286	0.19470351964054736	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC	252	0.17155694737558716	No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	238	0.1620260058547212	No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT	230	0.15657975355708353	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	185	0.12594458438287154	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0020423446116141332	0.0
3	0.0	0.0	0.0	0.003403907686023555	0.0
4	0.0	0.0	0.0	0.005446252297637688	0.0
5	0.0	0.0	0.0	0.005446252297637688	0.0
6	0.0	0.0	0.0	0.006127033834842399	0.0
7	0.0	0.0	0.0	0.006127033834842399	0.0
8	0.0	0.0	0.0	0.006127033834842399	0.0
9	0.0	0.0	0.0	0.012934849206889509	0.0
10	0.0	0.0	0.0	0.019061883041731908	0.0
11	0.0	0.0	0.0	0.019061883041731908	0.0
12	0.0	0.0	0.0	0.02042344611614133	0.0
13	0.0	0.0	0.0	0.023827353802164886	0.0
14	0.0	0.0	0.0	0.029273606099802573	0.0
15	0.0	0.0	0.0	0.031996732248621415	0.0
16	0.0	0.0	0.0	0.031996732248621415	0.0
17	0.0	0.0	0.0	0.03471985839744026	0.0
18	0.0	0.0	0.0	0.037442984546259106	0.0
19	0.0	0.0	0.0	0.052420178364762746	0.0
20	0.0	0.0	0.0	0.05650486758799101	0.0
21	0.0	0.0	0.0	0.06127033834842399	0.0
22	0.0	0.0	0.0	0.07012049833208524	0.0
23	0.0	0.0	0.0	0.07692831370413235	0.0
24	0.0	0.0	0.0	0.08305534753897474	0.0
25	0.0	0.0	0.0	0.08986316291102185	0.0
26	0.0	0.0	0.0	0.09599019674586426	0.0
27	0.0	0.0	0.0	0.13819865205255633	0.0
28	0.0	0.0	0.0	0.1851725781196814	0.0
29	0.0	0.0	0.0	0.24303900878208182	0.0
30	0.0	0.0	0.0	0.2981823132956634	0.0
31	0.0	0.0	0.0	0.34447545782558375	0.0
32	0.0	0.0	0.0	0.4350194022738103	0.0
33	0.0	0.0	0.0	0.5596024235822724	0.0
34	0.0	0.0	0.0	0.7318401524950643	0.0
35	0.0	0.0	0.0	0.9701136905167131	0.0
36	0.0	0.0	0.0	1.3738171420791068	0.0
37	0.0	0.0	0.0	1.9306964395125603	0.0
38	0.0	0.0	0.0	2.5760773367826264	0.0
39	0.0	0.0	0.0	3.158826332629859	0.0
40	0.0	0.0	0.0	3.8484580298182314	0.0
41	0.0	0.0	0.0	4.540812853155423	0.0
42	0.0	0.0	0.0	5.228402205732181	0.0
43	0.0	0.0	0.0	5.997685342773504	0.0
44	0.0	0.0	0.0	6.718632990673293	0.0
45	0.0	0.0	0.0	7.528763019946899	0.0
46	0.0	0.0	0.0	8.272857240111648	0.0
47	0.0	0.0	0.0	9.025801620260058	0.0
48	0.0	0.0	0.0	9.682755803662605	0.0
49	0.0	0.0	0.0	10.383280005446252	0.0
50	0.0	0.0	0.0	10.986452447409626	0.0
51	0.0	0.0	0.0	11.657703043093472	0.0
52	0.0	0.0	0.0	12.309891755735585	0.0
53	0.0	0.0	0.0	12.860644019334195	0.0
54	0.0	0.0	0.0	13.487643815099734	0.0
55	0.0	0.0	0.0	14.000272312614882	0.0
56	0.0	0.0	0.0	14.503369868609163	0.0
57	0.0	0.0	0.0	15.033017904554429	0.0
58	0.0	0.0	0.0	15.57355844509497	0.0
59	0.0	0.0	0.0	16.10116413642862	0.0
60	0.0	0.0	0.0	16.579072775546326	0.0
61	0.0	0.0	0.0	17.08829736537545	0.0
62	0.0	0.0	0.0	17.571652256790795	0.0
63	0.0	0.0	0.0	18.101981074273265	0.0
64	0.0	0.0	0.0	18.555381578051602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAT	25	2.3841517E-4	56.000004	39
CTATACA	125	0.0	56.0	1
TCTTTAT	115	0.0	54.782608	1
TCGTTGT	20	0.0069400296	52.5	34
TGCCCAT	20	0.0069400296	52.5	12
GGTAAGC	20	0.0069400296	52.5	16
GTCTACT	20	0.0069400296	52.5	27
TATAACT	20	0.0069400296	52.5	4
GCCAAGA	20	0.0069400296	52.5	1
ATATTAG	20	0.0069400296	52.5	58
TCTCGTA	4050	0.0	50.728394	38
AATCTCG	4015	0.0	50.5604	36
ATCTCGT	4065	0.0	50.541206	37
TAATCTC	4020	0.0	50.49751	35
TATGCCG	4230	0.0	50.472816	43
CGTCTTC	4210	0.0	50.46318	48
TCGTATG	4215	0.0	50.40332	40
CTCGTAT	4170	0.0	50.35971	39
CGTATGC	4230	0.0	50.30733	41
CTGCTTG	4190	0.0	50.286396	54
>>END_MODULE