FastQCFastQC Report
Wed 25 May 2016
SRR1779748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35006
Sequences flagged as poor quality0
Sequence length76
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAAAC207.7710196E-570.0916966
TTACAAC150.002195395570.0916963
GTACCCC150.002207922769.9914238
CGGTTTT150.002207922769.9914246
TAGTTCT150.002207922769.9914239
TCGTGTA401.2914825E-1069.9914234
ACGATAG150.002207922769.9914224
CTCGGTT353.5779522E-969.9914244
TTCCAAT150.002207922769.991424
CTACGAA150.002207922769.9914222
AGGCAAC150.002207922769.9914227
GTGTCCG150.002207922769.9914248
TTGGTCT150.002207922769.9914248
GTGTACA207.826475E-569.9914236
AGATTAT150.002207922769.9914256
TCCCGTG150.002207922769.9914232
CATGTGA150.002207922769.991429
CATCGGT207.826475E-569.9914243
ATCGGTG309.9635145E-869.9914244
AAGGCGA150.002207922769.9914245
Produced by FastQC (version 0.11.5)