FastQCFastQC Report
Wed 25 May 2016
SRR1779748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35006
Sequences flagged as poor quality0
Sequence length76
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1643846.9576644003885No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG20405.82757241615723No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC11303.228018054047878No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT10162.9023595954979147Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC6231.7796949094440955No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC5421.548306004684911No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC4211.2026509741187226No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT3971.1340912986345197No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2110.6027538136319488No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1990.5684739758898475No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1900.5427640975832714No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1890.5399074444380964No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT1640.4684911158087185No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG1450.4142147060503914No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT1420.405644746614866No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT1310.3742215620179398No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG820.23424555790435925No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT800.228532251614009No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT740.21139233274295838No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCTAAGATCTCGTATGCCGT600.17139918871050677No Hit
CTTATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC480.1371193509684054No Hit
CTCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG480.1371193509684054No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCC380.1085528195166543No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCG370.10569616637147919No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG370.10569616637147919No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGTCTT360.10283951322630407No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGG150.002206876470.070
TTTCTAA150.002206876470.029
ACTTTCT150.002206876470.027
TCTAAGA150.002206876470.031
TTCTAAG150.002206876470.030
CAGATCT150.002206876470.034
TCTTTAT750.070.01
GACTTTC150.002206876470.026
TCTTAAT150.002206876470.01
CTTTCTA150.002206876470.028
TTTACAC352.8928298E-760.02
AAACCAC252.3590405E-455.99999669
TCTGCTC252.3590405E-455.99999653
CTGCTCG252.3590405E-455.99999654
TTTATAC1000.055.9999963
CTTTATA950.055.2631572
CTTATAC23600.053.093221
AAACTCA200.006896135452.50000469
AAATCTC200.006896135452.50000468
CTTTACA407.276103E-752.5000041
Produced by FastQC (version 0.11.5)