##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779748_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35006
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.60469633777067	34.0	34.0	34.0	34.0	34.0
2	33.6126092669828	34.0	34.0	34.0	34.0	34.0
3	33.61632291607153	34.0	34.0	34.0	34.0	34.0
4	33.65037422156202	34.0	34.0	34.0	34.0	34.0
5	33.699794320973545	34.0	34.0	34.0	34.0	34.0
6	37.37862080786151	38.0	38.0	38.0	37.0	38.0
7	37.3360566759984	38.0	38.0	38.0	37.0	38.0
8	37.22904644918014	38.0	38.0	38.0	36.0	38.0
9	37.25961263783351	38.0	38.0	38.0	36.0	38.0
10-11	37.14461806547449	38.0	38.0	38.0	36.0	38.0
12-13	37.1362337884934	38.0	38.0	38.0	36.0	38.0
14-15	37.13846197794663	38.0	38.0	38.0	36.0	38.0
16-17	36.90051705421928	38.0	37.5	38.0	34.5	38.0
18-19	36.83311432325887	38.0	37.5	38.0	34.0	38.0
20-21	36.994229560646744	38.0	38.0	38.0	34.5	38.0
22-23	36.98927326743987	38.0	37.5	38.0	35.0	38.0
24-25	36.82805804719191	38.0	37.5	38.0	34.5	38.0
26-27	36.68038336285208	38.0	37.0	38.0	34.0	38.0
28-29	36.768225447066214	38.0	37.0	38.0	34.0	38.0
30-31	36.524195852139634	38.0	37.0	38.0	34.5	38.0
32-33	36.38287722104782	38.0	37.0	38.0	34.0	38.0
34-35	35.97299034451237	38.0	37.0	38.0	32.5	38.0
36-37	35.952193909615495	38.0	37.0	38.0	32.0	38.0
38-39	33.16527166771411	37.0	33.0	38.0	22.5	38.0
40-41	35.52502428155174	37.5	36.0	38.0	30.5	38.0
42-43	36.59856881677427	38.0	37.0	38.0	34.0	38.0
44-45	36.6700137119351	38.0	37.0	38.0	34.0	38.0
46-47	36.66338627663829	38.0	37.0	38.0	34.0	38.0
48-49	36.57852939496087	38.0	37.0	38.0	34.0	38.0
50-51	36.31420327943781	38.0	37.0	38.0	33.5	38.0
52-53	36.34935439638919	38.0	37.0	38.0	34.0	38.0
54-55	36.36285208250014	38.0	37.0	38.0	34.0	38.0
56-57	36.31390333085757	38.0	37.0	38.0	34.0	38.0
58-59	36.0444352396732	38.0	37.0	38.0	32.5	38.0
60-61	36.05030566188653	38.0	37.0	38.0	33.0	38.0
62-63	36.27165343084043	38.0	37.0	38.0	34.0	38.0
64-65	36.39781751699709	38.0	37.0	38.0	34.0	38.0
66-67	36.1387190767297	38.0	37.0	38.0	32.5	38.0
68-69	35.291278637947784	38.0	36.0	38.0	28.0	38.0
70-71	30.625349940010288	34.5	27.5	38.0	16.5	38.0
72-73	23.022553276581156	22.5	11.0	35.5	10.0	38.0
74-75	22.018653945037993	21.5	11.0	34.0	10.5	38.0
76	22.141832828657943	22.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	15.0
24	16.0
25	36.0
26	82.0
27	128.0
28	299.0
29	453.0
30	686.0
31	1080.0
32	1622.0
33	2601.0
34	4535.0
35	10730.0
36	7558.0
37	5162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.866537165057418	6.898817345597898	13.317716962806376	67.91692852653831
2	6.610295377935212	4.536365194538079	70.65074558647089	18.20259384105582
3	6.781694566645719	9.73833057190196	70.41078672227619	13.069188139176141
4	5.593326858252871	56.695994972290464	23.81877392447009	13.891904244986575
5	6.08467119922299	22.68468262583557	64.52608124321544	6.70456493172599
6	10.335371079243558	65.07455864708908	11.817974061589442	12.77209621207793
7	9.306975946980518	20.927840941552876	10.312517854082158	59.45266525738445
8	8.087185053990744	65.7715820145118	7.153059475518482	18.988173455978973
9	6.990230246243501	15.780151973947323	4.076444038164886	73.1531737416443
10-11	8.962749242986916	38.130606181797404	33.98274581500314	18.923898760212534
12-13	9.789750328515112	7.195909272696109	39.97457578700794	43.03976461178084
14-15	11.480888990458778	5.890418785351083	11.669428098040335	70.9592641261498
16-17	40.84442666971376	32.24875735588185	4.1235788150602755	22.783237159344115
18-19	41.985659601211225	13.69479517796949	3.9064731760269664	40.41307204479232
20-21	13.106324630063417	6.667428440838713	4.0750157115922985	76.15123121750557
22-23	12.037936353767925	36.756556018968176	3.912186482317317	47.29332114494658
24-25	40.99440095983546	37.38930469062446	3.8293435411072387	17.786950808432838
26-27	41.884248414557504	39.948865908701364	7.58155744729475	10.58532822944638
28-29	13.463406273210307	12.122207621550592	34.22556133234303	40.188824772896076
30-31	35.913843341141515	5.8875621322059075	44.31954522081929	13.879049305833286
32-33	17.328457978632233	8.645660743872478	35.513911900817	38.51196937667829
34-35	10.502485288236302	64.16899960006856	13.733359995429357	11.595155116265783
36-37	38.389133291435755	43.33828486545164	8.215734445523625	10.056847397588985
38-39	12.839227560989544	12.51499742901217	35.913843341141515	38.73193166885677
40-41	10.108267154202137	5.653316574301549	40.4387819230989	43.79963434839741
42-43	39.580357652973774	5.953265154544935	38.163457692966915	16.302919499514367
44-45	12.679254984859739	34.37696394903731	41.10295377935211	11.840827286750843
46-47	39.56178940753014	7.5986973661658	14.780323373136033	38.05918985316803
48-49	38.48483117179912	4.264983145746443	9.038450551334057	48.21173513112038
50-51	14.5546477746672	4.404959149860024	39.54607781523167	41.4943152602411
52-53	8.447123350282808	4.463520539336114	68.97246186368051	18.116894246700564
54-55	8.8727646689139	4.207850082842941	46.01925384219848	40.90013140604468
56-57	38.61480888990459	4.399245843569674	17.141347197623265	39.84459806890248
58-59	12.612123635948121	7.968633948465978	63.25344226703994	16.165800148545966
60-61	37.43072616122951	38.18773924470091	15.737302176769695	8.644232417299891
62-63	10.021139233274296	76.5154544935154	4.712049362966349	8.751356910243958
64-65	6.566017254184997	80.1762554990573	4.480660458207165	8.777066788550535
66-67	6.877392447009084	80.27766668571103	4.29640633034337	8.548534536936526
68-69	6.864537507855796	79.28783637090785	4.809175569902303	9.038450551334057
70-71	7.575844141004399	72.50471347768953	7.181626006970234	12.737816374335829
72-73	9.984002742387018	55.51905387647832	13.083471404902017	21.413471976232646
74-75	11.188082043078328	48.088899045877845	16.000114266125806	24.722904644918014
76	10.752442438439125	50.05141975661315	15.514483231446038	23.681654573501685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	3.5
21	6.0
22	4.5
23	4.0
24	6.5
25	8.0
26	8.5
27	11.5
28	13.5
29	13.0
30	15.5
31	23.5
32	32.5
33	36.0
34	33.5
35	44.0
36	69.0
37	81.0
38	244.5
39	3616.0
40	7336.5
41	6931.0
42	6013.0
43	4735.5
44	2534.5
45	1157.0
46	703.0
47	490.5
48	206.0
49	133.0
50	132.0
51	120.0
52	107.5
53	110.0
54	113.0
55	143.5
56	209.5
57	238.5
58	232.0
59	252.5
60	306.0
61	368.5
62	398.0
63	378.5
64	373.5
65	383.0
66	401.5
67	425.0
68	477.0
69	490.0
70	449.0
71	447.0
72	419.5
73	408.0
74	376.5
75	329.0
76	328.0
77	283.5
78	208.5
79	177.0
80	171.5
81	137.5
82	97.5
83	86.0
84	57.5
85	25.5
86	17.5
87	13.0
88	7.0
89	1.5
90	1.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	35006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.990858709935438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.87767933695342	14.368965320230817
2	18.705344384109747	7.478717934068444
3	5.587310660188625	3.350854139290407
4	1.557587882252072	1.2455007712963493
5	0.685910260074307	0.6855967548420271
6	0.2857959416976279	0.34279837742101354
7	0.17147756501857675	0.23995886419470946
8	0.15718776793369535	0.2513854767754099
9	0.08573878250928837	0.1542592698394561
>10	0.6001714775650185	2.539564646060675
>50	0.05715918833952558	0.8455693309718335
>100	0.14289797084881395	6.253213734788322
>500	0.02857959416976279	3.328000914129006
>1k	0.04286939125464419	11.957950065703022
>5k	0.0	0.0
>10k+	0.014289797084881395	46.9576644003885
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	16438	46.9576644003885	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	2040	5.82757241615723	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1130	3.228018054047878	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	1016	2.9023595954979147	Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	623	1.7796949094440955	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	542	1.548306004684911	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	421	1.2026509741187226	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	397	1.1340912986345197	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	211	0.6027538136319488	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	199	0.5684739758898475	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	190	0.5427640975832714	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	189	0.5399074444380964	No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT	164	0.4684911158087185	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG	145	0.4142147060503914	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT	142	0.405644746614866	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT	131	0.3742215620179398	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	82	0.23424555790435925	No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	80	0.228532251614009	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT	74	0.21139233274295838	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCTAAGATCTCGTATGCCGT	60	0.17139918871050677	No Hit
CTTATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	48	0.1371193509684054	No Hit
CTCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	48	0.1371193509684054	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCC	38	0.1085528195166543	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCG	37	0.10569616637147919	No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	37	0.10569616637147919	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGTCTT	36	0.10283951322630407	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005713306290350226	0.0
2	0.0	0.0	0.0	0.005713306290350226	0.0
3	0.0	0.0	0.0	0.005713306290350226	0.0
4	0.0	0.0	0.0	0.014283265725875564	0.0
5	0.0	0.0	0.0	0.017139918871050677	0.0
6	0.0	0.0	0.0	0.017139918871050677	0.0
7	0.0	0.0	0.0	0.017139918871050677	0.0
8	0.0	0.0	0.0	0.017139918871050677	0.0
9	0.0	0.0	0.0	0.017139918871050677	0.0
10	0.0	0.0	0.0	0.017139918871050677	0.0
11	0.0	0.0	0.0	0.017139918871050677	0.0
12	0.0	0.0	0.0	0.017139918871050677	0.0
13	0.0	0.0	0.0	0.02856653145175113	0.0
14	0.0	0.0	0.0	0.02856653145175113	0.0
15	0.0	0.0	0.0	0.02856653145175113	0.0
16	0.0	0.0	0.0	0.02856653145175113	0.0
17	0.0	0.0	0.0	0.02856653145175113	0.0
18	0.0	0.0	0.0	0.02856653145175113	0.0
19	0.0	0.0	0.0	0.02856653145175113	0.0
20	0.0	0.0	0.0	0.02856653145175113	0.0
21	0.0	0.0	0.0	0.03427983774210135	0.0
22	0.0	0.0	0.0	0.03427983774210135	0.0
23	0.0	0.0	0.0	0.04284979717762669	0.0
24	0.0	0.0	0.0	0.04284979717762669	0.0
25	0.0	0.0	0.0	0.06284636919385249	0.0
26	0.0	0.0	0.0	0.0657030223390276	0.0
27	0.0	0.0	0.0	0.0885562475004285	0.0
28	0.0	0.0	0.0	0.1314060446780552	0.0
29	0.0	0.0	0.0	0.1599725761298063	0.0
30	0.0	0.0	0.0	0.21424898588813346	0.0
31	0.0	0.0	0.0	0.25424212992058504	0.0
32	0.0	0.0	0.0	0.3085185396789122	0.0
33	0.0	0.0	0.0	0.37993486830829	0.0
34	0.0	0.0	0.0	0.5399074444380964	0.0
35	0.0	0.0	0.0	0.6884534079872022	0.0
36	0.0	0.0	0.0	0.8969890875849854	0.0
37	0.0	0.0	0.0	1.2226475461349482	0.0
38	0.0	0.0	0.0	1.5740158829914872	0.0
39	0.0	0.0	0.0	1.8825344226703993	0.0
40	0.0	0.0	0.0	2.1339198994458095	0.0
41	0.0	0.0	0.0	2.39673198880192	0.0
42	0.0	0.0	0.0	2.6452608124321544	0.0
43	0.0	0.0	0.0	2.842369879449237	0.0
44	0.0	0.0	0.0	3.1023253156601727	0.0
45	0.0	0.0	0.0	3.3794206707421584	0.0
46	0.0	0.0	0.0	3.5793863909044163	0.0
47	0.0	0.0	0.0	3.7622121921956238	0.0
48	0.0	0.0	0.0	3.9907444438096324	0.0
49	0.0	0.0	0.0	4.150717019939439	0.0
50	0.0	0.0	0.0	4.356396046392047	0.0
51	0.0	0.0	0.0	4.55921841969948	0.0
52	0.0	0.0	0.0	4.722047648974462	0.0
53	0.0	0.0	0.0	4.9305833285722445	0.0
54	0.0	0.0	0.0	5.073415985831001	0.0
55	0.0	0.0	0.0	5.213391989944581	0.0
56	0.0	0.0	0.0	5.396217791235788	0.0
57	0.0	0.0	0.0	5.55904702051077	0.0
58	0.0	0.0	0.0	5.7304462092212765	0.0
59	0.0	0.0	0.0	5.853282294463806	0.0
60	0.0	0.0	0.0	5.996114951722562	0.0
61	0.0	0.0	0.0	6.181797406158944	0.0
62	0.0	0.0	0.0	6.316060103982174	0.0
63	0.0	0.0	0.0	6.45889276124093	0.0
64	0.0	0.0	0.0	6.573158887047935	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGG	15	0.0022068764	70.0	70
TTTCTAA	15	0.0022068764	70.0	29
ACTTTCT	15	0.0022068764	70.0	27
TCTAAGA	15	0.0022068764	70.0	31
TTCTAAG	15	0.0022068764	70.0	30
CAGATCT	15	0.0022068764	70.0	34
TCTTTAT	75	0.0	70.0	1
GACTTTC	15	0.0022068764	70.0	26
TCTTAAT	15	0.0022068764	70.0	1
CTTTCTA	15	0.0022068764	70.0	28
TTTACAC	35	2.8928298E-7	60.0	2
AAACCAC	25	2.3590405E-4	55.999996	69
TCTGCTC	25	2.3590405E-4	55.999996	53
CTGCTCG	25	2.3590405E-4	55.999996	54
TTTATAC	100	0.0	55.999996	3
CTTTATA	95	0.0	55.263157	2
CTTATAC	2360	0.0	53.09322	1
AAACTCA	20	0.0068961354	52.500004	69
AAATCTC	20	0.0068961354	52.500004	68
CTTTACA	40	7.276103E-7	52.500004	1
>>END_MODULE