FastQCFastQC Report
Wed 25 May 2016
SRR1779747_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779747_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77962
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCTT150.00221823969.9910338
GTCGTAT150.00221823969.9910354
CAGTCGA150.00221823969.9910318
GTTGGTC301.01270416E-769.9910347
CGTTGGT207.887426E-569.9910246
GATATCG207.887426E-569.9910241
CGTGTAT207.887426E-569.9910235
TATCGGT207.887426E-569.9910243
CCGTCTC601.8189894E-1258.32585555
ATATCGG308.313662E-658.32585542
GTAGATA308.313662E-658.32585538
ATCGGTG308.313662E-658.32585544
CGACTAG308.313662E-658.32585522
TGTCGTA308.313662E-658.32585553
ATGTATA252.3786958E-455.99281328
CCGACTA509.640644E-1055.99281321
TTCGATC252.3786958E-455.99281338
TATGTAT252.3786958E-455.99281327
AGTGTAC252.3786958E-455.99281335
TGTGTAG452.6628186E-854.4374635
Produced by FastQC (version 0.11.5)