##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779747_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77962
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.13650239860445	32.0	21.0	34.0	12.0	34.0
2	25.512198250429698	27.0	21.0	34.0	12.0	34.0
3	24.948167055745106	27.0	21.0	34.0	11.0	34.0
4	25.79614427541623	31.0	21.0	34.0	12.0	34.0
5	25.687360508965906	31.0	21.0	34.0	11.0	34.0
6	27.40333752340884	31.0	21.0	37.0	11.0	38.0
7	28.353916010364024	34.0	21.0	37.0	12.0	38.0
8	27.559926630922757	33.0	21.0	37.0	11.0	38.0
9	29.094571714424976	34.0	21.0	38.0	12.0	38.0
10-11	28.45269490264488	34.0	21.0	38.0	11.0	38.0
12-13	28.20133526589877	32.5	21.0	37.5	11.0	38.0
14-15	27.567648341499705	32.5	16.0	37.0	11.0	38.0
16-17	27.53758882532516	31.0	16.0	37.5	11.0	38.0
18-19	28.006317180164693	33.0	21.0	37.0	11.0	38.0
20-21	27.057200944049665	30.5	16.0	37.0	11.0	38.0
22-23	27.09773992457864	30.5	16.0	37.5	11.0	38.0
24-25	27.667190426105023	32.5	21.0	37.0	11.0	38.0
26-27	26.50954311074626	29.0	11.0	37.0	11.0	38.0
28-29	27.956959800928658	34.0	21.0	37.5	11.0	38.0
30-31	28.430190349144453	34.0	21.0	38.0	11.0	38.0
32-33	28.57193247992612	34.0	21.0	38.0	11.0	38.0
34-35	28.61697365383135	34.0	21.0	38.0	11.0	38.0
36-37	27.197160154947284	30.5	16.0	37.0	11.0	38.0
38-39	26.261422231343474	27.0	16.0	36.5	11.0	38.0
40-41	26.542129498986686	29.0	11.0	37.0	11.0	38.0
42-43	27.353120751135165	32.5	16.0	37.0	11.0	38.0
44-45	28.734248736563966	34.0	21.0	38.0	11.0	38.0
46-47	27.703919858392553	30.5	16.0	37.5	11.0	38.0
48-49	26.95930709832996	30.5	16.0	37.0	11.0	38.0
50-51	25.412771606680177	26.5	11.0	36.5	11.0	38.0
52-53	27.618128062389367	32.5	21.0	37.0	11.0	38.0
54-55	27.032631281906568	30.5	16.0	37.0	11.0	38.0
56-57	27.416664528873042	32.0	16.0	37.5	11.0	38.0
58-59	28.175399553628694	34.0	21.0	38.0	11.0	38.0
60-61	28.73687822272389	34.0	21.0	38.0	11.0	38.0
62-63	28.688662425284114	34.0	21.0	38.0	11.0	38.0
64-65	28.477418485929043	34.0	20.5	38.0	11.0	38.0
66-67	27.089646237910777	31.0	18.0	37.5	11.0	38.0
68-69	26.97024832610759	31.0	11.0	37.0	11.0	38.0
70-71	26.697634745132245	29.0	11.0	37.0	11.0	38.0
72-73	25.87804314922655	27.0	11.0	36.5	11.0	38.0
74-75	26.802608963341115	30.5	15.0	37.0	11.0	38.0
76	26.881916831276776	31.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	1.0
8	0.0
9	0.0
10	0.0
11	8.0
12	50.0
13	295.0
14	849.0
15	1424.0
16	2497.0
17	3942.0
18	4993.0
19	5097.0
20	4330.0
21	3420.0
22	2546.0
23	1979.0
24	1693.0
25	1485.0
26	1570.0
27	1633.0
28	1908.0
29	2126.0
30	2436.0
31	2973.0
32	3514.0
33	4456.0
34	5694.0
35	6823.0
36	6887.0
37	3332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.2597932326005	15.660193427567275	12.38295579897899	38.69705754085324
2	17.275082732613324	13.35650701623868	43.446807418999	25.92160283214899
3	19.490264487827403	12.363715656345398	41.39324286190708	26.752776993920115
4	16.608091121315514	19.31838588030066	25.340550524614557	38.73297247376927
5	15.310022831635925	21.833713860598753	39.107513917036506	23.748749390728815
6	27.29919704471409	32.023293399348404	13.955516790231137	26.72199276570637
7	26.038326364126114	21.42069213206434	16.419537723506323	36.121443780303224
8	21.476122676722976	31.661984838573133	16.966175395390003	29.89571708931389
9	21.337865572088248	14.18419702411493	16.50590046177527	47.97203694202155
10-11	24.34469179509874	20.656238174949813	25.84162289393852	29.15744713601293
12-13	22.68613831540736	14.67813415940328	30.240701902911127	32.39502562227823
14-15	25.56534677595208	19.278228858018753	21.508189992432115	33.64823437359705
16-17	29.433156314938813	16.591626260294003	22.34023448523564	31.634982939531543
18-19	23.1836839404823	16.0287326834274	27.103001539250897	33.684581836839406
20-21	28.838321970741593	16.104309233522105	23.399028995452824	31.658339800283475
22-23	30.660595177013857	15.053232426885582	22.392252437147256	31.89391995895331
24-25	24.275425060448054	21.179586836754982	18.81297340285661	35.73201469994035
26-27	27.761031298101592	20.930605438686506	22.58786557208825	28.720497691123654
28-29	25.29598132399533	20.449968574030606	22.52151716884083	31.732532933133236
30-31	24.925922600341206	19.673161533626647	23.89653535832938	31.504380507702766
32-33	24.631221139045664	19.4465110312981	24.62480759363776	31.29746023601847
34-35	24.162877354557757	20.3500490633077	23.931349850244676	31.555723731889866
36-37	28.016188100078885	15.693606213562344	29.155897047788248	27.13430863857052
38-39	27.564312239047194	13.971356922504633	34.2010915924294	24.263239246018767
40-41	25.013628871401178	21.311065360860447	23.63983863416261	30.035467133575764
42-43	20.36800687527658	21.6186402088237	26.202371713880744	31.810981202018972
44-45	20.01564925152965	16.805628599648532	30.614810349029618	32.5639117997922
46-47	26.28206772703951	16.63160595177014	27.59427911749615	29.4920472036942
48-49	25.82126502392283	17.26035480188304	29.178799112353932	27.739581061840195
50-51	32.795874781136355	15.715211103201021	26.830597938673286	24.658316176989334
52-53	19.01371866161276	20.349027379600948	28.55457000109031	32.082683957695984
54-55	25.04056593487644	17.787440914833986	25.75119132370013	31.420801826589447
56-57	25.195613134941	18.421626475115442	24.760133401744486	31.622626988199077
58-59	20.116214188136148	20.780661753859377	27.165387156151592	31.937736901852887
60-61	19.890328373524884	18.613391482811696	26.949717804002056	34.546562339661364
62-63	20.000128271730837	20.442665743110204	26.427824703852643	33.12938128130632
64-65	20.226780056695016	21.40878131373379	25.360757301727787	33.00368132784341
66-67	18.611303522409504	28.325508607198746	19.12311757613074	33.94007029426101
68-69	19.19889148341074	27.89253547509687	19.25534371712299	33.653229324369406
70-71	18.455855000705498	30.2710399055914	18.640567477776777	32.632537615926324
72-73	16.921715260585692	31.699354789055782	18.517425826395925	32.8615041239626
74-75	18.960087738989333	25.954834944233152	24.631053701648955	30.454023615128563
76	20.24782257340397	23.327646583460538	26.53959132364448	29.884939519491017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.5
16	4.0
17	5.0
18	4.0
19	5.5
20	14.5
21	21.0
22	24.0
23	38.0
24	56.5
25	64.0
26	81.0
27	134.0
28	192.0
29	214.0
30	270.5
31	404.5
32	555.5
33	629.0
34	704.0
35	910.0
36	1155.0
37	1269.0
38	1447.0
39	1774.5
40	2107.0
41	2386.5
42	2483.0
43	2617.0
44	2786.5
45	2938.0
46	3054.0
47	3093.5
48	3016.0
49	2881.5
50	2864.0
51	2870.5
52	2829.5
53	2701.5
54	2621.0
55	2636.0
56	2688.0
57	2658.0
58	2591.0
59	2607.5
60	2612.5
61	2561.5
62	2522.0
63	2465.0
64	2396.5
65	2374.0
66	2324.0
67	2285.0
68	2215.0
69	2065.5
70	1892.5
71	1799.0
72	1738.5
73	1559.0
74	1339.5
75	1239.0
76	1105.0
77	860.5
78	638.5
79	527.0
80	454.5
81	320.0
82	212.5
83	167.0
84	120.0
85	59.0
86	30.0
87	15.0
88	13.0
89	6.5
90	2.5
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.001282676175572715
9	0.00256535235114543
10-11	0.003206690438931787
12-13	0.003206690438931787
14-15	0.001282676175572715
16-17	0.00513070470229086
18-19	0.00256535235114543
20-21	0.0019240142633590725
22-23	0.00256535235114543
24-25	0.003206690438931787
26-27	0.00256535235114543
28-29	0.001282676175572715
30-31	0.003848028526718145
32-33	0.00256535235114543
34-35	0.0019240142633590725
36-37	0.004489366614504502
38-39	0.003206690438931787
40-41	0.003206690438931787
42-43	0.0019240142633590725
44-45	0.003848028526718145
46-47	0.00256535235114543
48-49	0.003848028526718145
50-51	0.003206690438931787
52-53	0.003206690438931787
54-55	0.003206690438931787
56-57	0.00256535235114543
58-59	0.003206690438931787
60-61	0.00256535235114543
62-63	0.003206690438931787
64-65	0.001282676175572715
66-67	0.00513070470229086
68-69	0.025653523511454297
70-71	0.003848028526718145
72-73	0.003848028526718145
74-75	0.004489366614504502
76	0.003848028526718145
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	77962.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.74169467176317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.87764513321468	97.63346245606834
2	0.9911536612930464	1.957363843923963
3	0.11691196529013653	0.346322567404633
4	0.01039217469245658	0.04104563761832688
5	0.0012990218365570726	0.006413380877863574
6	0.002598043673114145	0.01539211410687258
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001282676175572715	0.0
2	0.0	0.0	0.0	0.00256535235114543	0.0
3	0.0	0.0	0.0	0.00256535235114543	0.0
4	0.0	0.0	0.0	0.011544085580154434	0.0
5	0.0	0.0	0.0	0.014109437931299864	0.0
6	0.0	0.0	0.0	0.015392114106872579	0.0
7	0.0	0.0	0.0	0.015392114106872579	0.0
8	0.0	0.0	0.0	0.015392114106872579	0.0
9	0.0	0.0	0.0	0.015392114106872579	0.0
10	0.0	0.0	0.0	0.016674790282445295	0.0
11	0.0	0.0	0.0	0.019240142633590723	0.0
12	0.0	0.0	0.0	0.02052281880916344	0.0
13	0.0	0.0	0.0	0.02052281880916344	0.0
14	0.0	0.0	0.0	0.021805494984736152	0.0
15	0.0	0.0	0.0	0.023088171160308868	0.0
16	0.0	0.0	0.0	0.023088171160308868	0.0
17	0.0	0.0	0.0	0.023088171160308868	0.0
18	0.0	0.0	0.0	0.026936199687027013	0.0
19	0.0	0.0	0.0	0.03334958056489059	0.0
20	0.0	0.0	0.0	0.035914932916036015	0.0
21	0.0	0.0	0.0	0.037197609091608734	0.0
22	0.0	0.0	0.0	0.046176342320617736	0.0
23	0.0	0.0	0.0	0.046176342320617736	0.0
24	0.0	0.0	0.0	0.0602857802519176	0.0
25	0.0	0.0	0.0	0.07311254200764475	0.0
26	0.0	0.0	0.0	0.07439521818321747	0.0
27	0.0	0.0	0.0	0.13468099843513506	0.0
28	0.0	0.0	0.0	0.21035889279392525	0.0
29	0.0	0.0	0.0	0.2770580539237064	0.0
30	0.0	0.0	0.0	0.32451707241989686	0.0
31	0.0	0.0	0.0	0.3591493291603602	0.0
32	0.0	0.0	0.0	0.4361098996947231	0.0
33	0.0	0.0	0.0	0.5040917370000769	0.0
34	0.0	0.0	0.0	0.5951617454657397	0.0
35	0.0	0.0	0.0	0.8170647238398194	0.0
36	0.0	0.0	0.0	0.999204740771145	0.0
37	0.0	0.001282676175572715	0.0	1.4827736589620584	0.0
38	0.0	0.001282676175572715	0.0	1.921448911007927	0.0
39	0.0	0.001282676175572715	0.0	2.411431210076704	0.0
40	0.0	0.001282676175572715	0.0	2.878325337985172	0.001282676175572715
41	0.0	0.001282676175572715	0.0	3.3939611605654036	0.001282676175572715
42	0.0	0.001282676175572715	0.0	3.86470331700059	0.001282676175572715
43	0.0	0.001282676175572715	0.0	4.323901387855622	0.001282676175572715
44	0.0	0.001282676175572715	0.0	4.7933608681152355	0.001282676175572715
45	0.0	0.001282676175572715	0.0	5.125573997588568	0.001282676175572715
46	0.0	0.001282676175572715	0.0	5.49498473615351	0.001282676175572715
47	0.0	0.001282676175572715	0.0	5.86311279854288	0.001282676175572715
48	0.0	0.001282676175572715	0.0	6.14658423334445	0.001282676175572715
49	0.0	0.001282676175572715	0.0	6.421076934917011	0.001282676175572715
50	0.0	0.001282676175572715	0.0	6.766116826146071	0.001282676175572715
51	0.0	0.001282676175572715	0.0	7.208640106718658	0.001282676175572715
52	0.0	0.001282676175572715	0.0	7.5485492932454274	0.001282676175572715
53	0.0	0.001282676175572715	0.0	7.934634822092814	0.001282676175572715
54	0.0	0.001282676175572715	0.0	8.257869218337138	0.001282676175572715
55	0.0	0.001282676175572715	0.0	8.502860367871527	0.001282676175572715
56	0.0	0.001282676175572715	0.0	8.776070393268515	0.001282676175572715
57	0.0	0.001282676175572715	0.0	9.018496190451758	0.001282676175572715
58	0.0	0.001282676175572715	0.0	9.249377902054848	0.001282676175572715
59	0.0	0.001282676175572715	0.0	9.51104384187168	0.001282676175572715
60	0.0	0.001282676175572715	0.0	9.688053154100716	0.001282676175572715
61	0.0	0.001282676175572715	0.0	9.85993176162746	0.001282676175572715
62	0.0	0.001282676175572715	0.0	9.957415150970986	0.001282676175572715
63	0.0	0.001282676175572715	0.0	10.11518432056643	0.001282676175572715
64	0.0	0.001282676175572715	0.0	10.270388137810729	0.001282676175572715
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGCTT	15	0.002218239	69.99103	38
GTCGTAT	15	0.002218239	69.99103	54
CAGTCGA	15	0.002218239	69.99103	18
GTTGGTC	30	1.01270416E-7	69.99103	47
CGTTGGT	20	7.887426E-5	69.99102	46
GATATCG	20	7.887426E-5	69.99102	41
CGTGTAT	20	7.887426E-5	69.99102	35
TATCGGT	20	7.887426E-5	69.99102	43
CCGTCTC	60	1.8189894E-12	58.325855	55
ATATCGG	30	8.313662E-6	58.325855	42
GTAGATA	30	8.313662E-6	58.325855	38
ATCGGTG	30	8.313662E-6	58.325855	44
CGACTAG	30	8.313662E-6	58.325855	22
TGTCGTA	30	8.313662E-6	58.325855	53
ATGTATA	25	2.3786958E-4	55.992813	28
CCGACTA	50	9.640644E-10	55.992813	21
TTCGATC	25	2.3786958E-4	55.992813	38
TATGTAT	25	2.3786958E-4	55.992813	27
AGTGTAC	25	2.3786958E-4	55.992813	35
TGTGTAG	45	2.6628186E-8	54.43746	35
>>END_MODULE