FastQCFastQC Report
Wed 25 May 2016
SRR1779747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77962
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1995525.595803083553527No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG24393.1284471922218517No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC15531.9919961006644262No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT10001.2826761755727147RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6190.7939765526795105No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC5690.7298427439008748No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT5250.6734049921756753No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC4050.5194838511069495No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG2460.31553833919088786No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT2460.31553833919088786No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC2300.2950155203817244No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT2110.27064467304584283RNA PCR Primer, Index 30 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2100.2693619968702701No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG2080.2667966445191247No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG1870.2398604448320977No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT1740.2231856545496524No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT1170.15007311254200764No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG840.10774479874810805No Hit
TCTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT780.10004874169467176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATT150.002217110770.000014
TATTACA150.002217110770.000013
TCTTAAT252.8162103E-670.01
TCTTTAT700.064.999991
TTTACAC650.064.615392
CTATACA900.054.4444471
CGGCCTT200.00692787152.50000439
ATTACAC200.00692787152.5000044
CTTATTA200.00692787152.5000041
CTTTACA800.052.5000041
ATTGCTA200.00692787152.50000434
TGGCTTC200.00692787152.50000427
TGCACCG200.00692787152.5000045
TCTGCTT31550.049.2551553
TCTCGTA30200.049.13907238
TGAAAAA31800.049.0880559
TGCTTGA31450.049.07790455
CTGCTTG31550.049.0332854
GCTTGAA31700.049.0220856
CTTCTGC31850.049.01099451
Produced by FastQC (version 0.11.5)