FastQCFastQC Report
Wed 25 May 2016
SRR1779746_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779746_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216706
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG150.002222665669.9983835
AAACGCG150.002222665669.9983832
CGCGTAT308.358404E-658.3319935
ACGCGTA252.3869668E-455.99870734
TCTCGGT2150.055.34756543
CTCTCGG452.6904672E-854.4431942
GCCGTAT1200.052.4987954
GGTCCGT200.00694509952.49878715
GGACCGT200.00694509952.4987876
GACTAGC200.00694509952.49878723
TAGATCC352.0821033E-549.99884439
AGATCTC1700.049.41062540
ATCTCGG2000.048.99886742
GTAGATC2000.048.99886738
TAGATCT1950.048.4604239
TCTCTCG451.6770173E-646.67636564
CTAAATT305.8694574E-446.66559220
AGTCGAG305.8694574E-446.66559224
GCCGACT1450.045.86100820
GATCTCG1950.044.87075841
Produced by FastQC (version 0.11.5)