##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779746_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	216706
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.97266342417838	34.0	23.0	34.0	12.0	34.0
2	27.57737672237963	34.0	23.0	34.0	12.0	34.0
3	26.541064852842098	32.0	21.0	34.0	11.0	34.0
4	28.689279484647404	33.0	23.0	34.0	12.0	34.0
5	28.330013935931632	34.0	23.0	34.0	11.0	34.0
6	30.596097939143355	36.0	26.0	38.0	11.0	38.0
7	31.55519920998957	37.0	27.0	38.0	12.0	38.0
8	30.763033787712384	36.0	26.0	38.0	11.0	38.0
9	32.19638588686977	37.0	31.0	38.0	12.0	38.0
10-11	31.87272156746929	37.0	28.0	38.0	11.0	38.0
12-13	31.028414072522217	36.0	27.0	38.0	11.0	38.0
14-15	30.91966996760588	36.5	26.5	38.0	11.0	38.0
16-17	30.635086245881517	35.5	26.5	38.0	11.0	38.0
18-19	31.199694516995375	36.5	26.5	38.0	11.5	38.0
20-21	30.09013133000471	35.0	24.0	38.0	11.0	38.0
22-23	30.09552112078115	35.5	24.5	38.0	11.0	38.0
24-25	31.21325667032754	36.5	27.0	38.0	11.0	38.0
26-27	29.822679113637832	35.0	24.0	38.0	11.0	38.0
28-29	31.820076509187565	37.0	29.0	38.0	11.0	38.0
30-31	32.25838001716612	37.0	31.0	38.0	11.0	38.0
32-33	32.37366985685676	37.0	31.0	38.0	11.0	38.0
34-35	32.42867987042352	37.0	31.0	38.0	11.0	38.0
36-37	30.45162339759859	36.0	25.0	38.0	11.0	38.0
38-39	28.79602779803051	34.0	22.0	37.5	11.0	38.0
40-41	29.8775945289932	35.0	24.5	37.5	11.0	38.0
42-43	30.85537317840761	36.0	25.5	38.0	11.0	38.0
44-45	32.45982806198259	37.0	31.0	38.0	11.0	38.0
46-47	31.11925142820226	36.5	28.0	38.0	11.0	38.0
48-49	30.54344826631473	35.5	25.0	38.0	11.0	38.0
50-51	28.470937583638662	34.0	21.0	37.5	11.0	38.0
52-53	31.632654379666462	36.5	29.0	38.0	11.0	38.0
54-55	30.735918710141853	36.5	26.0	38.0	11.0	38.0
56-57	31.164026376750066	36.5	27.0	38.0	11.0	38.0
58-59	32.202098695929045	37.0	31.0	38.0	11.0	38.0
60-61	32.65092567810767	38.0	33.0	38.0	11.0	38.0
62-63	32.56144269194208	38.0	32.0	38.0	11.0	38.0
64-65	32.29610624532777	37.5	30.5	38.0	11.0	38.0
66-67	30.303900215037885	36.0	24.0	38.0	11.0	38.0
68-69	30.377135381576885	36.0	24.5	38.0	11.0	38.0
70-71	30.01078881064668	35.0	24.0	38.0	11.0	38.0
72-73	28.826737607634307	34.0	22.0	37.5	11.0	38.0
74-75	30.78488135999926	35.5	25.5	38.0	11.0	38.0
76	31.187456738622835	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	13.0
4	3.0
5	1.0
6	0.0
7	4.0
8	1.0
9	2.0
10	5.0
11	8.0
12	83.0
13	399.0
14	1141.0
15	2079.0
16	3259.0
17	5192.0
18	6656.0
19	7045.0
20	6140.0
21	4935.0
22	3960.0
23	3318.0
24	3248.0
25	3356.0
26	3728.0
27	4158.0
28	5084.0
29	6222.0
30	7737.0
31	9599.0
32	12212.0
33	16042.0
34	21604.0
35	28875.0
36	32554.0
37	18042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.030271427648515	18.71337203399998	11.10029256227331	30.15606397607819
2	20.949678133868623	17.49475092868185	37.74163032694216	23.813940610507373
3	20.04531526875369	16.654053455404608	37.08284111045481	26.21779016538689
4	14.52508294839433	18.879008403283787	31.699899862021862	34.896008786300015
5	14.57135473867821	28.600500216883706	39.01322529140864	17.81491975302945
6	27.82492328280381	33.01815832583466	16.863939456865324	22.292978934496205
7	27.876016353954203	25.122516220132347	19.572600666340573	27.428866759572877
8	23.905097698974554	31.52903279399684	18.15807204895563	26.407797458072974
9	22.910070979592216	16.14254991185239	19.87059377336373	41.076785335191666
10-11	24.976232231862657	23.0348901606055	26.552750599963083	25.436127007568764
12-13	24.546165685579986	18.94347233150961	28.927019611734273	27.58334237117613
14-15	24.24083697147024	21.988037990456235	23.304458986736567	30.46666605133696
16-17	25.64538647036426	21.96887006888276	25.470468102781847	26.91527535797113
18-19	22.44997796284374	22.07061581737082	27.597673060903315	27.881733158882128
20-21	25.117798812089546	21.62560053165223	26.789826613070705	26.466774043187513
22-23	25.933925452107836	20.807229110275777	26.764183967564996	26.49466147005139
24-25	23.141443649134466	23.98251856364249	23.43887727459423	29.437160512628815
26-27	24.071189749340643	23.197354728100475	26.359959111812582	26.371496410746303
28-29	23.4699740409576	24.050995096625325	25.490395154312083	26.98863570810499
30-31	23.189335499338892	23.748681821013793	25.867459843133815	27.194522836513503
32-33	23.304566536677665	23.496088792486788	25.950573413018894	27.248771257816646
34-35	23.536999305448195	23.310173914849333	25.951086015972386	27.201740763730086
36-37	24.806679173964515	20.760268329998823	29.894057187427165	24.538995308609497
38-39	24.286205472213183	19.835561309140584	33.04488488599364	22.833348332652594
40-41	23.647519799147112	22.537567612472078	25.661586885488006	28.1533257028928
42-43	21.129240304496612	23.086685541949045	27.103105653124	28.68096850043035
44-45	20.79615284325467	21.492803887769725	29.674242371797977	28.036800897177628
46-47	23.24755518430149	20.379079115574363	29.78198566569597	26.591380034428173
48-49	23.00608046520751	21.289243230146184	30.300331137289287	25.404345167357018
50-51	25.453601467585063	19.876547483068546	31.142339598260126	23.527511451086266
52-53	19.296367148108576	22.984832156842835	29.70455819878113	28.01424249626746
54-55	21.69455386255547	22.141533576553062	28.62770074281244	27.536211818079025
56-57	21.82851103793765	22.38738811497823	27.972236131333805	27.811864715750318
58-59	19.40972863208418	23.433634853239802	28.83330256599594	28.32333394868008
60-61	19.657646306821462	22.651971948965393	28.696236093900353	28.99414565031279
62-63	19.57882203084762	23.346394188611672	28.01553457203777	29.05924920850294
64-65	20.217231281858396	23.656380447800114	26.993075089474644	29.13331318086685
66-67	19.232207058562903	25.18195621974127	23.0234039312697	32.56243279042613
68-69	19.545212195071286	25.256602893894385	23.271868659940868	31.92631625109346
70-71	19.271995550930082	25.973522495725156	22.675718647095067	32.0787633062497
72-73	18.86389281816147	26.384712834914403	21.905957443862683	32.84543690306145
74-75	19.770814632604225	26.49638523078911	25.348381603328402	28.38441853327826
76	20.379087863096395	25.68004135168314	26.547688274767168	27.3931825104533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	2.0
3	3.0
4	2.0
5	4.0
6	4.0
7	1.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	2.0
14	3.5
15	4.5
16	6.5
17	9.0
18	14.0
19	29.0
20	43.5
21	48.0
22	69.5
23	125.0
24	195.5
25	232.0
26	311.0
27	494.0
28	724.5
29	851.0
30	1066.0
31	1497.0
32	2017.0
33	2321.0
34	2790.0
35	3740.5
36	4727.0
37	5232.0
38	5938.0
39	7343.5
40	8668.5
41	9977.5
42	10661.0
43	11002.0
44	11749.5
45	12456.0
46	12756.0
47	12611.5
48	12106.0
49	11343.0
50	10941.0
51	10361.5
52	9129.0
53	7932.0
54	7388.0
55	7011.0
56	6175.5
57	5510.0
58	5303.0
59	5117.0
60	4692.0
61	4201.0
62	3949.0
63	3939.5
64	3759.0
65	3455.0
66	3209.5
67	3097.0
68	3017.5
69	2874.5
70	2694.0
71	2577.0
72	2481.5
73	2218.0
74	1931.0
75	1812.0
76	1639.5
77	1293.5
78	969.0
79	818.0
80	708.0
81	514.0
82	358.5
83	287.0
84	206.0
85	108.5
86	64.5
87	37.0
88	31.0
89	15.0
90	4.5
91	2.5
92	1.0
93	2.0
94	2.5
95	1.5
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.6145468976401205E-4
3	9.229093795280241E-4
4	0.00230727344882006
5	0.0
6	4.6145468976401205E-4
7	0.0
8	0.00922909379528024
9	0.01107491255433629
10-11	0.011997821933864315
12-13	0.011305639899218296
14-15	0.00922909379528024
16-17	0.015458732107094404
18-19	0.012228549278746321
20-21	0.010613457864572278
22-23	0.012228549278746321
24-25	0.00876763910551623
26-27	0.008075457070870212
28-29	0.008536911760634223
30-31	0.011305639899218296
32-33	0.009690548485044253
34-35	0.008998366450398235
36-37	0.014997277417330392
38-39	0.013612913348038356
40-41	0.013843640692920363
42-43	0.009459821140162248
44-45	0.012228549278746321
46-47	0.010613457864572278
48-49	0.013151458658274346
50-51	0.010844185209454283
52-53	0.014074368037802367
54-55	0.013612913348038356
56-57	0.00992127582992626
58-59	0.011997821933864315
60-61	0.013612913348038356
62-63	0.012920731313392338
64-65	0.011305639899218296
66-67	0.015228004762212399
68-69	0.035762738456710935
70-71	0.01453582272756638
72-73	0.012690003968510331
74-75	0.011767094588982308
76	0.012920731313392338
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	216706.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.88444441903884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.67801519801715	91.73239759599251
2	2.311355755620649	4.386234134536259
3	0.5604941446921212	1.5954652655770885
4	0.21184500150804794	0.804031810841663
5	0.09660053270454262	0.4582943938126859
6	0.05375964064304947	0.30605721807497577
7	0.03563358541675759	0.23667510684491863
8	0.019419824892351317	0.14741114365006203
9	0.013555272298235941	0.11575660328702146
>10	0.019321044207116982	0.21767672738282903
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0023072734488200604	0.0
2	0.0	0.0	0.0	0.0032301828283480844	0.0
3	0.0	0.0	0.0	0.0032301828283480844	0.0
4	0.0	0.0	0.0	0.005998910966932157	0.0
5	0.0	0.0	0.0	0.006921820346460181	0.0
6	0.0	0.0	0.0	0.006921820346460181	0.0
7	0.0	0.0	0.0	0.007383275036224193	0.0
8	0.0	0.0	0.0	0.007844729725988205	0.0
9	0.0	0.0	0.0	0.00876763910551623	0.0
10	0.0	0.0	0.0	0.009229093795280242	0.0
11	0.0	0.0	0.0	0.010152003174808265	0.0
12	0.0	0.0	0.0	0.010152003174808265	0.0
13	0.0	0.0	0.0	0.011536367244100301	0.0
14	0.0	0.0	0.0	0.013843640692920363	0.0
15	0.0	0.0	0.0	0.014766550072448386	0.0
16	0.0	0.0	0.0	0.014766550072448386	4.6145468976401205E-4
17	0.0	0.0	0.0	0.017073823521268447	4.6145468976401205E-4
18	0.0	0.0	0.0	0.018458187590560483	4.6145468976401205E-4
19	0.0	0.0	0.0	0.020765461039380543	4.6145468976401205E-4
20	0.0	0.0	0.0	0.024918553247256652	4.6145468976401205E-4
21	0.0	0.0	0.0	0.025380007937020665	4.6145468976401205E-4
22	0.0	0.0	0.0	0.03137891890395282	4.6145468976401205E-4
23	0.0	0.0	0.0	0.03645492049135696	4.6145468976401205E-4
24	0.0	0.0	0.0	0.03922364862994102	4.6145468976401205E-4
25	0.0	0.0	0.0	0.045684014286637195	4.6145468976401205E-4
26	0.0	0.0	0.0	0.05122147056380534	4.6145468976401205E-4
27	0.0	0.0	0.0	0.09229093795280241	4.6145468976401205E-4
28	0.0	0.0	0.0	0.15089568355283195	4.6145468976401205E-4
29	0.0	0.0	0.0	0.18827351342371693	4.6145468976401205E-4
30	0.0	0.0	0.0	0.23949498398752225	4.6145468976401205E-4
31	0.0	0.0	0.0	0.27687281385840723	4.6145468976401205E-4
32	0.0	0.0	0.0	0.343783743874189	4.6145468976401205E-4
33	0.0	0.0	0.0	0.40700303637185864	4.6145468976401205E-4
34	0.0	0.0	0.0	0.48360451487268463	9.229093795280241E-4
35	0.0	0.0	0.0	0.6714165736066375	9.229093795280241E-4
36	0.0	0.0	0.0	0.8140060727437173	9.229093795280241E-4
37	0.0	0.0	0.0	1.2445432982935405	9.229093795280241E-4
38	0.0	0.0	0.0	1.6930772567441603	9.229093795280241E-4
39	0.0	0.0	0.0	2.2403625188042784	9.229093795280241E-4
40	0.0	0.0	0.0	2.894243814199884	9.229093795280241E-4
41	0.0	0.0	0.0	3.7354757136396777	9.229093795280241E-4
42	0.0	0.0	0.0	4.583167978736168	9.229093795280241E-4
43	0.0	0.0	0.0	5.420246785968086	9.229093795280241E-4
44	0.0	0.0	0.0	6.260094321338587	9.229093795280241E-4
45	0.0	0.0	0.0	6.791690123946729	9.229093795280241E-4
46	0.0	0.0	0.0	7.43126632395965	9.229093795280241E-4
47	0.0	0.0	0.0	8.212970568419887	9.229093795280241E-4
48	0.0	0.0	0.0	8.748719463235904	9.229093795280241E-4
49	0.0	0.0	0.0	9.207405424861333	9.229093795280241E-4
50	0.0	0.0	0.0	9.918507101787675	9.229093795280241E-4
51	0.0	0.0	0.0	10.685906250865228	9.229093795280241E-4
52	0.0	0.0	0.0	11.415004660692366	9.229093795280241E-4
53	0.0	0.0	0.0	12.369754413814107	9.229093795280241E-4
54	0.0	0.0	0.0	13.165302298967264	9.229093795280241E-4
55	0.0	0.0	0.0	13.663673363912398	9.229093795280241E-4
56	0.0	0.0	0.0	14.219726265078032	9.229093795280241E-4
57	0.0	0.0	0.0	14.670106042287708	9.229093795280241E-4
58	0.0	0.0	0.0	15.065111256725702	9.229093795280241E-4
59	0.0	0.0	0.0	15.514106669866086	9.229093795280241E-4
60	0.0	0.0	0.0	15.818666765110335	9.229093795280241E-4
61	0.0	0.0	0.0	16.14260795732467	9.229093795280241E-4
62	0.0	0.0	0.0	16.39410076324606	9.229093795280241E-4
63	0.0	0.0	0.0	16.742499054017888	9.229093795280241E-4
64	0.0	0.0	0.0	17.080283886925145	9.229093795280241E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	15	0.0022226656	69.99838	35
AAACGCG	15	0.0022226656	69.99838	32
CGCGTAT	30	8.358404E-6	58.33199	35
ACGCGTA	25	2.3869668E-4	55.998707	34
TCTCGGT	215	0.0	55.347565	43
CTCTCGG	45	2.6904672E-8	54.44319	42
GCCGTAT	120	0.0	52.49879	54
GGTCCGT	20	0.006945099	52.498787	15
GGACCGT	20	0.006945099	52.498787	6
GACTAGC	20	0.006945099	52.498787	23
TAGATCC	35	2.0821033E-5	49.998844	39
AGATCTC	170	0.0	49.410625	40
ATCTCGG	200	0.0	48.998867	42
GTAGATC	200	0.0	48.998867	38
TAGATCT	195	0.0	48.46042	39
TCTCTCG	45	1.6770173E-6	46.676365	64
CTAAATT	30	5.8694574E-4	46.665592	20
AGTCGAG	30	5.8694574E-4	46.665592	24
GCCGACT	145	0.0	45.861008	20
GATCTCG	195	0.0	44.870758	41
>>END_MODULE