FastQCFastQC Report
Wed 25 May 2016
SRR1779746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216706
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2813912.984873515269534No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG30481.4065138944007087No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC21981.0142774081012984No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT12010.5542070824065785Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC8610.3973124878868144No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC7010.32347973752457243Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC4240.19565678845994114Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT3500.16150914141740422No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG2870.13243749596227147No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2560.11813240057958709Illumina PCR Primer Index 10 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT2510.11582512713076702Illumina PCR Primer Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA150.00222246270.030
AATGCGT150.00222246270.08
GAGTATT252.3866948E-456.00000429
GTGGATA200.006944464552.512
TATACGG200.006944464552.54
CAATCGC200.006944464552.553
CAGAGCG200.006944464552.54
TCGAATG200.006944464552.516
AATGTCC200.006944464552.529
ATCGAAT200.006944464552.568
TGCGTGG200.006944464552.538
AAATGCG200.006944464552.57
GCGCTAT352.0818188E-550.019
TCTCGTA39450.049.41698538
CCGTCTT40700.049.27518547
TCTTCTG40600.049.22413650
CTTTATA1850.049.189192
TATGCCG40800.049.1544143
CTCGTAT40700.049.10319539
CGTATGC40800.049.0686341
Produced by FastQC (version 0.11.5)