##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779746_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	216706
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.690474652293894	34.0	34.0	34.0	34.0	34.0
2	33.68657074561849	34.0	34.0	34.0	34.0	34.0
3	33.705296576929115	34.0	34.0	34.0	34.0	34.0
4	33.74413260361965	34.0	34.0	34.0	34.0	34.0
5	33.74758428469909	34.0	34.0	34.0	34.0	34.0
6	37.42499054017886	38.0	38.0	38.0	37.0	38.0
7	37.4112207322363	38.0	38.0	38.0	37.0	38.0
8	37.315242771312285	38.0	38.0	38.0	36.0	38.0
9	37.301182246915175	38.0	38.0	38.0	36.0	38.0
10-11	37.2343659151108	38.0	38.0	38.0	36.0	38.0
12-13	37.21728055522228	38.0	38.0	38.0	36.0	38.0
14-15	37.235609535499705	38.0	38.0	38.0	36.0	38.0
16-17	37.20931123273006	38.0	38.0	38.0	36.0	38.0
18-19	37.20801223777838	38.0	38.0	38.0	36.0	38.0
20-21	37.220395374378185	38.0	38.0	38.0	36.0	38.0
22-23	37.18900953365389	38.0	38.0	38.0	36.0	38.0
24-25	37.183617435604	38.0	38.0	38.0	36.0	38.0
26-27	37.08777560381346	38.0	38.0	38.0	36.0	38.0
28-29	37.05497771173849	38.0	38.0	38.0	36.0	38.0
30-31	36.97043459802682	38.0	38.0	38.0	35.5	38.0
32-33	36.99271824499553	38.0	38.0	38.0	35.5	38.0
34-35	36.78683792788386	38.0	37.5	38.0	34.5	38.0
36-37	36.83913689514827	38.0	37.0	38.0	35.0	38.0
38-39	36.49984541267893	38.0	37.0	38.0	34.0	38.0
40-41	36.88430408018237	38.0	37.0	38.0	35.0	38.0
42-43	37.022528217954275	38.0	38.0	38.0	36.0	38.0
44-45	37.019152676898656	38.0	38.0	38.0	36.0	38.0
46-47	37.036385702287895	38.0	38.0	38.0	36.0	38.0
48-49	37.01953568429116	38.0	38.0	38.0	36.0	38.0
50-51	36.95859367068748	38.0	38.0	38.0	35.0	38.0
52-53	36.95304929258997	38.0	38.0	38.0	35.5	38.0
54-55	36.96131163881019	38.0	38.0	38.0	36.0	38.0
56-57	36.966724502321114	38.0	38.0	38.0	36.0	38.0
58-59	36.90940490803208	38.0	38.0	38.0	35.0	38.0
60-61	36.94785792733012	38.0	38.0	38.0	35.5	38.0
62-63	36.96159774071784	38.0	38.0	38.0	36.0	38.0
64-65	36.98375448764686	38.0	38.0	38.0	36.0	38.0
66-67	36.93080717654334	38.0	38.0	38.0	35.0	38.0
68-69	36.75964209574262	38.0	38.0	38.0	35.0	38.0
70-71	35.619666737423046	38.0	37.0	38.0	28.5	38.0
72-73	33.691919005472855	38.0	35.5	38.0	20.0	38.0
74-75	33.44346026413666	38.0	35.5	38.0	15.0	38.0
76	33.05354258765332	38.0	34.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	7.0
22	17.0
23	33.0
24	52.0
25	98.0
26	184.0
27	369.0
28	630.0
29	1064.0
30	1656.0
31	2664.0
32	4179.0
33	6858.0
34	11812.0
35	26312.0
36	35412.0
37	125356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.27956770924663	15.44673428516054	10.718208079148708	37.555489926444125
2	19.21497328177346	15.746679833507148	40.9896357276679	24.04871115705149
3	17.562042583038775	17.74939318708296	37.938958773638014	26.74960545624025
4	13.139922291030242	26.544258119295268	31.184185024872406	29.131634564802084
5	13.436637656548504	29.42142810997388	42.11050916910469	15.03142506437293
6	27.36241728424686	42.46306055208439	15.507646304209391	14.66687585945936
7	25.94898156949969	25.109595488818954	17.850913218831042	31.090509722850314
8	22.650503447066534	41.538766808487075	15.53071903869759	20.2800107057488
9	20.89466835251447	15.32352588299355	16.241820715623934	47.53998504886805
10-11	23.2997701955645	27.358494919383862	27.0516275506908	22.29010733436084
12-13	22.720644559910664	18.175777320424906	28.268252840253616	30.835325279410814
14-15	21.043948944653124	18.129401124103627	19.274962391442784	41.55168753980047
16-17	29.24215296300056	27.258128524360192	18.904875730252048	24.594842782387197
18-19	29.426273384216405	21.5520105580833	19.675505062157946	29.34621099554235
20-21	21.57139165505339	20.450287486271723	19.68150397312488	38.29681688555001
22-23	22.01415742988196	27.915240002584145	19.370022057534168	30.700580509999725
24-25	29.148246933633587	28.252332653456758	19.302880400173507	23.29654001273615
26-27	29.191162219781642	29.437348296770736	19.88039094441317	21.491098539034454
28-29	21.96824268825044	28.989275793009885	20.712393750057682	28.330087768681995
30-31	29.07187618247764	22.016233975985898	27.134689394848323	21.777200446688138
32-33	28.243795741696122	28.341854863270978	22.451847203123126	20.962502191909778
34-35	22.485302668131016	28.54351056269785	28.172962446817344	20.79822432235379
36-37	20.605566989377312	27.447094219818556	30.391636595202716	21.555702195601416
38-39	20.289701254233847	21.5520105580833	29.590781981117274	28.56750620656558
40-41	20.100735558775483	19.197668730907314	30.285040561867234	30.416555148449977
42-43	27.75003922364863	18.901645547423698	29.95279318523714	23.39552204369053
44-45	20.003368619235275	26.49303664873146	31.4061447306489	22.097450001384363
46-47	26.68269452622447	19.2465829280223	24.906324697977904	29.164397847775327
48-49	25.970208485228834	18.718217308242505	23.853054368591547	31.458519837937114
50-51	19.308417856450674	19.421935710132622	31.2778603268945	29.9917861065222
52-53	17.403532897104835	19.51468810277519	38.872020156340845	24.20975884377913
54-55	17.137734995800763	19.418936254649154	32.74459405830941	30.698734691240663
56-57	24.534622945372995	19.482617001836587	24.89294251197475	31.089817540815666
58-59	18.018651998560262	19.86100984744308	37.03819921921866	25.08213893477799
60-61	24.597611510525784	27.505698965418585	24.11193044954916	23.78475907450647
62-63	17.52420329847812	37.39836460457948	20.856598340608937	24.220833756333466
64-65	17.464214188808803	38.7534263010715	19.639280869011472	24.14307864110823
66-67	17.28263176838666	38.56399915092337	18.947560289055218	25.205808791634748
68-69	17.209952654748832	38.98530728267791	19.07561396546473	24.729126097108526
70-71	17.9014425073602	37.664854687918194	19.250043838195527	25.183658966526078
72-73	18.68545402526926	34.084427750039225	20.34507581700553	26.885042407685987
74-75	19.231124195915204	32.77781879597242	20.913357267449907	27.077699740662464
76	19.33818168394045	32.99770195564498	21.223224091626445	26.44089226878813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	8.5
16	9.5
17	8.0
18	14.5
19	33.0
20	59.5
21	74.0
22	93.5
23	138.0
24	213.5
25	264.0
26	357.5
27	481.0
28	626.5
29	742.0
30	984.0
31	1341.0
32	1676.5
33	1897.0
34	2351.5
35	3164.5
36	4107.5
37	4692.0
38	11007.5
39	18349.5
40	17732.5
41	14539.0
42	12989.0
43	11775.0
44	10277.5
45	9890.5
46	9787.0
47	9762.0
48	9449.5
49	8691.5
50	8221.0
51	7933.0
52	6878.0
53	5585.5
54	5060.0
55	4848.5
56	4342.5
57	3809.5
58	3571.0
59	3426.5
60	3211.0
61	3154.5
62	3169.0
63	3127.5
64	3102.0
65	3076.0
66	3008.0
67	2982.0
68	2872.5
69	2768.0
70	2758.0
71	2743.0
72	2641.0
73	2448.5
74	2200.5
75	2043.0
76	1946.0
77	1693.5
78	1386.5
79	1235.0
80	1112.0
81	802.0
82	527.5
83	440.0
84	367.0
85	237.0
86	145.5
87	111.0
88	83.5
89	45.0
90	21.5
91	6.5
92	4.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	216706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.889287790831816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.12198651347206	14.389080136221425
2	24.528984458657714	15.644236892379537
3	13.440222267241628	12.857973475584433
4	6.338089312071311	8.084686164665492
5	3.2746794779035104	5.221359814679796
6	1.6930512545943914	3.239411922143365
7	1.137383150522386	2.5389237030815948
8	0.7510201719098197	1.9159598719001782
9	0.5990796747026308	1.719380174060709
>10	3.060515729459092	15.989866455012782
>50	0.031835151795791966	0.6451136562900889
>100	0.014470523543541806	1.073343608391092
>500	0.0028941047087083613	0.7207922254113869
>1k	0.004341157063062541	2.9749983849085857
>5k	0.0	0.0
>10k+	0.0014470523543541807	12.984873515269534
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	28139	12.984873515269534	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	3048	1.4065138944007087	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	2198	1.0142774081012984	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	1201	0.5542070824065785	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	861	0.3973124878868144	No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	701	0.32347973752457243	Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	424	0.19565678845994114	Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT	350	0.16150914141740422	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	287	0.13243749596227147	No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	256	0.11813240057958709	Illumina PCR Primer Index 10 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT	251	0.11582512713076702	Illumina PCR Primer Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006460365656696169	0.0
2	0.0	0.0	0.0	0.008306184415752217	0.0
3	0.0	0.0	0.0	0.008306184415752217	0.0
4	0.0	0.0	0.0	0.012920731313392338	0.0
5	0.0	0.0	0.0	0.015228004762212399	0.0
6	0.0	0.0	0.0	0.015228004762212399	0.0
7	0.0	0.0	0.0	0.01568945945197641	0.0
8	0.0	0.0	0.0	0.016150914141740424	0.0
9	0.0	0.0	0.0	0.020765461039380543	0.0
10	0.0	0.0	0.0	0.021688370418908566	0.0
11	0.0	0.0	0.0	0.023534189177964616	0.0
12	0.0	0.0	0.0	0.023534189177964616	0.0
13	0.0	0.0	0.0	0.025380007937020665	0.0
14	0.0	0.0	0.0	0.027225826696076712	0.0
15	0.0	0.0	0.0	0.030456009524424798	0.0
16	0.0	0.0	0.0	0.030456009524424798	0.0
17	0.0	0.0	0.0	0.03322473766300887	0.0
18	0.0	0.0	0.0	0.03507055642206492	0.0
19	0.0	0.0	0.0	0.03645492049135696	0.0
20	0.0	0.0	0.0	0.03968510331970504	0.0
21	0.0	0.0	0.0	0.04060801269923306	0.0
22	0.0	0.0	0.0	0.04245383145828911	0.0
23	0.0	0.0	0.0	0.045684014286637195	0.0
24	0.0	0.0	0.0	0.04845274242522127	0.0
25	0.0	0.0	0.0	0.06045056435908558	0.0
26	0.0	0.0	0.0	0.06737238470554577	0.0
27	0.0	0.0	0.0	0.12366985685675523	0.0
28	0.0	0.0	0.0	0.19750260721899718	0.0
29	0.0	0.0	0.0	0.27318117634029515	0.0
30	0.0	0.0	0.0	0.3479368360820651	0.0
31	0.0	0.0	0.0	0.4517641412789678	0.0
32	0.0	0.0	0.0	0.5680507230994989	0.0
33	0.0	0.0	0.0	0.7078714940979945	0.0
34	0.0	0.0	0.0	0.8919919153138354	0.0
35	0.0	0.0	0.0	1.1799396417265788	0.0
36	0.0	0.0	0.0	1.6520077893551632	0.0
37	0.0	0.0	0.0	2.301735992542892	0.0
38	0.0	0.0	0.0	3.05713731968658	0.0
39	0.0	0.0	0.0	3.855453932978321	0.0
40	0.0	0.0	0.0	4.829584783070151	0.0
41	0.0	0.0	0.0	5.961071682371508	0.0
42	0.0	0.0	0.0	7.162238239827231	0.0
43	0.0	0.0	0.0	8.315413509547497	0.0
44	0.0	0.0	0.0	9.42105894622207	0.0
45	0.0	0.0	0.0	10.465792363847793	0.0
46	0.0	0.0	0.0	11.513755964301865	0.0
47	0.0	0.0	0.0	12.610633761870922	0.0
48	0.0	0.0	0.0	13.58107297444464	0.0
49	0.0	0.0	0.0	14.476756527276587	0.0
50	0.0	0.0	0.0	15.351674619069154	0.0
51	0.0	0.0	0.0	16.271815270458593	0.0
52	0.0	0.0	0.0	17.311472686496913	0.0
53	0.0	0.0	0.0	18.372357018264378	0.0
54	0.0	0.0	0.0	19.28834457744594	0.0
55	0.0	0.0	0.0	20.11527138150305	0.0
56	0.0	0.0	0.0	20.914049449484555	0.0
57	0.0	0.0	0.0	21.673603868836118	0.0
58	0.0	0.0	0.0	22.371784814449068	0.0
59	0.0	0.0	0.0	23.033972294260426	0.0
60	0.0	0.0	0.0	23.71415650697258	0.0
61	0.0	0.0	0.0	24.394802174374497	0.0
62	0.0	0.0	0.0	25.155740957795356	0.0
63	0.0	0.0	0.0	25.903297555213054	0.0
64	0.0	0.0	0.0	26.64485524166382	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	15	0.002222462	70.0	30
AATGCGT	15	0.002222462	70.0	8
GAGTATT	25	2.3866948E-4	56.000004	29
GTGGATA	20	0.0069444645	52.5	12
TATACGG	20	0.0069444645	52.5	4
CAATCGC	20	0.0069444645	52.5	53
CAGAGCG	20	0.0069444645	52.5	4
TCGAATG	20	0.0069444645	52.5	16
AATGTCC	20	0.0069444645	52.5	29
ATCGAAT	20	0.0069444645	52.5	68
TGCGTGG	20	0.0069444645	52.5	38
AAATGCG	20	0.0069444645	52.5	7
GCGCTAT	35	2.0818188E-5	50.0	19
TCTCGTA	3945	0.0	49.416985	38
CCGTCTT	4070	0.0	49.275185	47
TCTTCTG	4060	0.0	49.224136	50
CTTTATA	185	0.0	49.18919	2
TATGCCG	4080	0.0	49.15441	43
CTCGTAT	4070	0.0	49.103195	39
CGTATGC	4080	0.0	49.06863	41
>>END_MODULE