FastQCFastQC Report
Wed 25 May 2016
SRR1779745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325828
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT2500.043.39839
GTAGATC1950.043.07493638
TTAATAC459.189882E-538.8870963
CCGTGTA852.568413E-937.05711734
CTTAAAG400.002417443834.9983942
CGTGTAT400.002417443834.9983935
AGATCTC3100.034.99838640
CGGTGGT1900.033.1563746
TCGGTGG2150.032.5566445
CTCGGTG2750.031.81671544
TCTCGGT2750.031.81671543
TGTAGAT2650.031.69665337
ATGGAAT703.4843448E-529.99862122
CGTATCA952.81525E-729.47232656
CTGTAGA604.977891E-429.16532536
CGTAACA604.977891E-429.16532556
ACATTTG1451.8189894E-1128.96418470
GACGCAA2100.028.3320369
CCCGTGT755.5777164E-527.9987133
CAGTGTA755.5777164E-527.9987134