FastQCFastQC Report
Wed 25 May 2016
SRR1779745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325828
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT244607.507028248032705No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG29340.900475097290595No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC17880.5487557852609353No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT9460.2903372331414121RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC7360.22588605030875183No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC6470.1985710252034816No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT4150.12736781369311417No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4130.12675399290423167RNA PCR Primer, Index 24 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATG200.00694758852.5000044
TCTCGTA33750.049.88148538
ATCTCGT34300.049.0816337
CTCGTAT35100.049.0598339
TCGTATG35200.049.0198940
CGTATGC35350.048.91089241
CCGTCTT35150.048.8904747
CTTGAAA35250.048.85106757
GTATGCC35450.048.7729242
TATGCCG35700.048.6274543
GCCGTCT35550.048.4388246
TCTTCTG35600.048.37078550
TTGAAAA35600.048.37078558
CTGCTTG35750.048.36363654
ATGCCGT35900.048.25905244
GTCTTCT35700.048.2352949
GCTTGAA35950.048.19193356
TGCTTGA36050.048.1553455
GCATCTC34800.047.9741435
TGAAAAA36050.047.8640859