##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779745_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325828
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.675021790638006	34.0	34.0	34.0	34.0	34.0
2	33.67905152411702	34.0	34.0	34.0	34.0	34.0
3	33.698761309648035	34.0	34.0	34.0	34.0	34.0
4	33.73507494751832	34.0	34.0	34.0	34.0	34.0
5	33.73194446149502	34.0	34.0	34.0	34.0	34.0
6	37.403485888260064	38.0	38.0	38.0	37.0	38.0
7	37.38510195563303	38.0	38.0	38.0	37.0	38.0
8	37.287065568336665	38.0	38.0	38.0	36.0	38.0
9	37.2768607977215	38.0	38.0	38.0	36.0	38.0
10-11	37.21134003216421	38.0	38.0	38.0	36.0	38.0
12-13	37.18420148053574	38.0	38.0	38.0	36.0	38.0
14-15	37.20622537044085	38.0	38.0	38.0	36.0	38.0
16-17	37.20347391875468	38.0	38.0	38.0	36.0	38.0
18-19	37.201862639183865	38.0	38.0	38.0	36.0	38.0
20-21	37.19749683882294	38.0	38.0	38.0	36.0	38.0
22-23	37.15285058374357	38.0	38.0	38.0	36.0	38.0
24-25	37.15686650625483	38.0	38.0	38.0	36.0	38.0
26-27	37.07708514921984	38.0	38.0	38.0	36.0	38.0
28-29	37.0204893379329	38.0	38.0	38.0	35.5	38.0
30-31	36.94038265587979	38.0	38.0	38.0	35.0	38.0
32-33	36.972528450593565	38.0	38.0	38.0	35.0	38.0
34-35	36.970106927581426	38.0	38.0	38.0	35.5	38.0
36-37	37.00522668401733	38.0	38.0	38.0	36.0	38.0
38-39	36.636062585167636	38.0	37.0	38.0	34.0	38.0
40-41	36.87515498974919	38.0	37.5	38.0	35.0	38.0
42-43	36.9902387149048	38.0	38.0	38.0	36.0	38.0
44-45	36.98988269884724	38.0	38.0	38.0	35.5	38.0
46-47	37.01837319076323	38.0	38.0	38.0	36.0	38.0
48-49	37.01157512552635	38.0	38.0	38.0	36.0	38.0
50-51	36.966336533385714	38.0	38.0	38.0	35.0	38.0
52-53	36.94533925875001	38.0	38.0	38.0	35.0	38.0
54-55	36.93718158046577	38.0	38.0	38.0	35.0	38.0
56-57	36.97465380507507	38.0	38.0	38.0	36.0	38.0
58-59	36.92697220619468	38.0	38.0	38.0	35.0	38.0
60-61	36.9774620351842	38.0	38.0	38.0	36.0	38.0
62-63	36.9748180021361	38.0	38.0	38.0	36.0	38.0
64-65	36.98579158328934	38.0	38.0	38.0	36.0	38.0
66-67	36.94797715359024	38.0	38.0	38.0	35.5	38.0
68-69	36.85040113188553	38.0	38.0	38.0	35.0	38.0
70-71	36.19082921050371	38.0	37.0	38.0	32.5	38.0
72-73	35.08564948377672	38.0	37.0	38.0	24.0	38.0
74-75	34.97120413224155	38.0	37.0	38.0	23.5	38.0
76	34.503980627815906	38.0	36.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	1.0
18	0.0
19	2.0
20	4.0
21	16.0
22	27.0
23	54.0
24	67.0
25	146.0
26	265.0
27	490.0
28	830.0
29	1392.0
30	2299.0
31	3779.0
32	5936.0
33	9706.0
34	16099.0
35	32366.0
36	47347.0
37	205000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.39309083320034	16.53326294854954	10.658077267760904	32.41556895048922
2	20.883103968965223	17.219514590520152	36.25440416416023	25.642977276354394
3	19.7189928428496	18.739641774187607	32.98366009059995	28.55770529236284
4	14.630725413408301	21.562603582258124	31.6823600181691	32.12431098616448
5	15.108891807947753	30.567968375952958	37.79662889622746	16.526510919871836
6	30.335944117755382	38.160317713640325	15.866346661428729	15.637391507175566
7	28.366500116625947	25.967688473673228	18.344341186147293	27.32147022355353
8	25.03161177062745	37.05789557680739	16.712191708508783	21.198300944056374
9	22.826153676172705	15.300403894079084	17.540849773500128	44.33259265624808
10-11	26.088457713885855	24.951661612875505	25.420313785187275	23.539566888051365
12-13	24.985421756264042	18.580355279472606	26.256491154842433	30.177731809420923
14-15	23.440281375449622	19.03980013995114	19.957615674527666	37.56230281007157
16-17	28.41575923493377	25.835563548866276	20.2280037320304	25.52067348416956
18-19	28.358366991173256	22.367783002074717	20.933130363259142	28.340719643492886
20-21	23.549541475870704	21.683526277667973	21.11021766085174	33.656714585609585
22-23	24.115023877628687	25.843543219121745	20.857476951029376	29.18395595222019
24-25	28.38875112022294	25.815460918030368	20.753894692905458	25.041893268841232
26-27	27.901530869047477	26.601611893391606	21.543268227408326	23.953589010152594
28-29	28.296984912285012	22.021588077144997	22.11212664350516	27.56930036706483
30-31	28.459340510944426	21.248634248744736	26.14400849527972	24.14801674503112
32-33	24.101366365076053	26.280890531200512	25.965079735320472	23.65266336840296
34-35	27.51344267527653	26.300993162036413	22.806511410928465	23.379052751758596
36-37	24.492523662791413	25.326399204488258	21.61401107332703	28.567066059393298
38-39	22.641853984310742	22.33218139631953	26.6640681586604	28.36189646070933
40-41	22.86451747547786	20.50683182538026	27.771707772198827	28.856942926943052
42-43	27.13272033097217	20.088666412954073	27.57896804448973	25.199645211584027
44-45	22.385737260149526	24.711964594816894	28.655149342597934	24.247148802435643
46-47	26.26907448101452	20.584633610371117	24.902555949764906	28.243735958849452
48-49	25.690088021901126	20.218489509802716	24.282290042599165	29.809132425696994
50-51	21.61615944608812	20.67962237745068	28.73555986594154	28.96865831051966
52-53	20.41920890776729	20.817271689357575	33.146015689259364	25.617503713615775
54-55	20.061044477454363	20.828473918754682	29.473679364572718	29.636802239218234
56-57	24.21246792786378	20.71906036313638	25.201947039542333	29.866524669457505
58-59	20.496857237560924	21.097020513890765	32.069067115165055	26.33705513338326
60-61	24.61881729010398	25.324557742121613	24.355488171673397	25.70113679610101
62-63	19.968664448727548	31.358262641639143	22.4086020845354	26.264470825097902
64-65	20.15158304381453	32.10666363848411	21.480965417336755	26.26078790036461
66-67	20.193015947064094	32.032698233423766	20.87757958186528	26.896706237646857
68-69	20.121505825159286	32.35341959561487	20.932823452864703	26.59225112636115
70-71	20.82878082914912	31.142658089544177	21.188326356237035	26.840234725069667
72-73	21.3637256466602	28.75044502007194	21.607105589452104	28.278723743815753
74-75	21.81427010569994	28.09334986557325	22.068698822691726	28.023681206035082
76	22.213560528867994	28.212124188222003	22.04905655744749	27.525258725462514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	3.5
14	5.5
15	7.0
16	8.5
17	8.0
18	10.0
19	30.0
20	55.5
21	63.0
22	98.5
23	188.0
24	302.5
25	363.0
26	471.5
27	657.0
28	979.5
29	1225.0
30	1382.5
31	1722.5
32	2448.5
33	2992.0
34	3403.5
35	4270.0
36	5423.5
37	6122.0
38	6696.0
39	8653.5
40	15800.0
41	22159.5
42	22756.0
43	21375.0
44	19081.5
45	16909.5
46	15650.0
47	15319.0
48	14187.0
49	12880.5
50	12375.0
51	11978.0
52	10710.5
53	9553.5
54	9267.0
55	9028.0
56	8628.5
57	8099.5
58	7731.0
59	7728.0
60	7952.5
61	8044.0
62	7908.0
63	8064.0
64	8104.0
65	7818.0
66	7357.5
67	7067.0
68	6677.0
69	5807.5
70	5101.5
71	4875.0
72	4411.5
73	3623.5
74	3014.0
75	2729.0
76	2434.0
77	1953.0
78	1566.5
79	1366.0
80	1178.0
81	859.0
82	624.5
83	521.0
84	413.5
85	243.0
86	155.5
87	131.0
88	87.0
89	39.0
90	25.5
91	13.0
92	10.0
93	5.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	325828.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.657760484652805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.5157716197698	18.1513729625029
2	24.938127919398948	18.28351840409919
3	11.85749123049209	13.040072204287503
4	5.30311789608507	7.776017026262496
5	2.4071294493737647	4.411998740534883
6	1.259745257011531	2.770766394192366
7	0.8283624883942698	2.1256139585819693
8	0.5680798724447115	1.6659628720184283
9	0.4676689294718773	1.542932604242466
>10	2.772517830877551	18.157388771817963
>50	0.0660919696709446	1.5870765949062657
>100	0.010875893743319997	0.8234397405455696
>500	0.0025098216330738453	0.7142611380747231
>1k	0.0016732144220492303	1.4481498900767895
>5k	0.0	0.0
>10k+	8.366072110246152E-4	7.501428697856475
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	24460	7.507028248032705	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2934	0.900475097290595	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1788	0.5487557852609353	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	946	0.2903372331414121	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	736	0.22588605030875183	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	647	0.1985710252034816	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	415	0.12736781369311417	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	413	0.12675399290423167	RNA PCR Primer, Index 24 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.003989835127736106	0.0
2	0.0	0.0	0.0	0.003989835127736106	0.0
3	0.0	0.0	0.0	0.003989835127736106	0.0
4	0.0	0.0	0.0	0.011048774199884601	0.0
5	0.0	0.0	0.0	0.01135568459432584	0.0
6	0.0	0.0	0.0	0.01135568459432584	3.069103944412389E-4
7	0.0	0.0	0.0	0.012583326172090797	3.069103944412389E-4
8	0.0	0.0	0.0	0.013504057355414514	3.069103944412389E-4
9	0.0	0.0	0.0	0.015959340510944425	3.069103944412389E-4
10	0.0	0.0	0.0	0.018414623666474336	3.069103944412389E-4
11	0.0	0.0	0.0	0.02056299642756301	3.069103944412389E-4
12	0.0	0.0	0.0	0.02056299642756301	3.069103944412389E-4
13	0.0	0.0	0.0	0.021483727610886724	3.069103944412389E-4
14	0.0	0.0	0.0	0.024859741949740354	3.069103944412389E-4
15	0.0	0.0	0.0	0.027008114710829027	3.069103944412389E-4
16	0.0	0.0	0.0	0.02854266668303522	3.069103944412389E-4
17	0.0	0.0	0.0	0.02854266668303522	3.069103944412389E-4
18	0.0	0.0	0.0	0.030077218655241416	3.069103944412389E-4
19	0.0	0.0	0.0	0.03161177062744761	3.069103944412389E-4
20	0.0	0.0	0.0	0.03590851614962495	3.069103944412389E-4
21	0.0	0.0	0.0	0.03774997851627239	3.069103944412389E-4
22	0.0	0.0	0.0	0.04173981364400849	3.069103944412389E-4
23	0.0	0.0	0.0	0.04327436561621469	3.069103944412389E-4
24	0.0	0.0	0.0	0.048184931927274514	3.069103944412389E-4
25	0.0	0.0	0.0	0.0512540358716869	3.069103944412389E-4
26	0.0	0.0	0.0	0.058926795732717877	3.069103944412389E-4
27	0.0	0.0	0.0	0.10158734056005009	3.069103944412389E-4
28	0.0	0.0	0.0	0.15192064524841328	3.069103944412389E-4
29	0.0	0.0	0.0	0.20164012914789398	3.069103944412389E-4
30	0.0	0.0	0.0	0.27222951986937893	3.069103944412389E-4
31	0.0	0.0	0.0	0.332690867574303	3.069103944412389E-4
32	0.0	0.0	0.0	0.4035871686902292	3.069103944412389E-4
33	0.0	0.0	0.0	0.5199062081834588	3.069103944412389E-4
34	0.0	0.0	0.0	0.6693715702763421	3.069103944412389E-4
35	0.0	0.0	0.0	0.8792982800741496	3.069103944412389E-4
36	0.0	0.0	0.0	1.2475907534036363	3.069103944412389E-4
37	0.0	0.0	0.0	1.7914359723535116	3.069103944412389E-4
38	0.0	0.0	0.0	2.3880697791472802	3.069103944412389E-4
39	0.0	0.0	0.0	3.0393336361515892	3.069103944412389E-4
40	0.0	0.0	0.0	3.7740771204439154	3.069103944412389E-4
41	0.0	0.0	0.0	4.659206698012448	3.069103944412389E-4
42	0.0	0.0	0.0	5.5676614655585155	3.069103944412389E-4
43	0.0	0.0	0.0	6.489927200854439	3.069103944412389E-4
44	0.0	0.0	0.0	7.380274255128473	3.069103944412389E-4
45	0.0	0.0	0.0	8.27645260689689	3.069103944412389E-4
46	0.0	0.0	0.0	9.1904317615429	3.069103944412389E-4
47	0.0	0.0	0.0	10.084768650944671	3.069103944412389E-4
48	0.0	0.0	0.0	10.936444995519109	3.069103944412389E-4
49	0.0	0.0	0.0	11.730115275544152	3.069103944412389E-4
50	0.0	0.0	0.0	12.505370931902721	3.069103944412389E-4
51	0.0	0.0	0.0	13.31960420835533	3.069103944412389E-4
52	0.0	0.0	0.0	14.183250058312975	3.069103944412389E-4
53	0.0	0.0	0.0	15.087101169942423	3.069103944412389E-4
54	0.0	0.0	0.0	15.961488883705513	3.069103944412389E-4
55	0.0	0.0	0.0	16.773880697791473	3.069103944412389E-4
56	0.0	0.0	0.0	17.461973802128732	3.069103944412389E-4
57	0.0	0.0	0.0	18.14331487778828	3.069103944412389E-4
58	0.0	0.0	0.0	18.817597014375682	3.069103944412389E-4
59	0.0	0.0	0.0	19.459653559546755	3.069103944412389E-4
60	0.0	0.0	0.0	20.085443853812443	3.069103944412389E-4
61	0.0	0.0	0.0	20.713689431233657	3.069103944412389E-4
62	0.0	0.0	0.0	21.28638422726101	3.069103944412389E-4
63	0.0	0.0	0.0	22.024196815497746	3.069103944412389E-4
64	0.0	0.0	0.0	22.72364560442933	3.069103944412389E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATG	20	0.006947588	52.500004	4
TCTCGTA	3375	0.0	49.881485	38
ATCTCGT	3430	0.0	49.08163	37
CTCGTAT	3510	0.0	49.05983	39
TCGTATG	3520	0.0	49.01989	40
CGTATGC	3535	0.0	48.910892	41
CCGTCTT	3515	0.0	48.89047	47
CTTGAAA	3525	0.0	48.851067	57
GTATGCC	3545	0.0	48.77292	42
TATGCCG	3570	0.0	48.62745	43
GCCGTCT	3555	0.0	48.43882	46
TCTTCTG	3560	0.0	48.370785	50
TTGAAAA	3560	0.0	48.370785	58
CTGCTTG	3575	0.0	48.363636	54
ATGCCGT	3590	0.0	48.259052	44
GTCTTCT	3570	0.0	48.23529	49
GCTTGAA	3595	0.0	48.191933	56
TGCTTGA	3605	0.0	48.15534	55
GCATCTC	3480	0.0	47.97414	35
TGAAAAA	3605	0.0	47.86408	59
>>END_MODULE