FastQCFastQC Report
Wed 25 May 2016
SRR1779744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105751
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCAT150.002219306870.0000156
GTCGTAT353.6707206E-970.0000154
CGTGTAC401.05483196E-861.2535
TACGCTT352.9500006E-760.00000414
CCGTGTA800.056.87534
GTGTAGC800.056.87536
TCGTGTA750.056.034
CTTAAAG252.3810889E-455.99999642
TTCGTGT509.677024E-1055.99999633
ATGTATA252.3810889E-455.99999628
TTTAGAT800.052.537
GAGTACT200.006934681452.536
TAGCACT200.006934681452.539
TAGATCG200.006934681452.541
GCGTCTA200.006934681452.536
CCGTTCA200.006934681452.536
GCGTACT200.006934681452.536
ACGACTT200.006934681452.524
TGTGTAG1000.052.49999635
AGTGTAG1000.052.49999635
Produced by FastQC (version 0.11.5)