##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779744_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105751
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.23472118466965	32.0	21.0	34.0	12.0	34.0
2	25.89064878819113	31.0	21.0	34.0	12.0	34.0
3	25.240196310200375	27.0	21.0	34.0	11.0	34.0
4	26.193671927452222	32.0	21.0	34.0	12.0	34.0
5	26.124358162097757	32.0	21.0	34.0	11.0	34.0
6	27.757477470662216	34.0	21.0	37.0	11.0	38.0
7	28.74416317576193	34.0	21.0	38.0	12.0	38.0
8	28.051318663653298	34.0	21.0	38.0	11.0	38.0
9	29.482491891329634	34.0	22.0	38.0	12.0	38.0
10-11	28.83103233066354	34.0	21.0	38.0	11.0	38.0
12-13	28.548462898695995	33.0	21.0	38.0	11.0	38.0
14-15	27.9479626670197	33.5	21.0	37.5	11.0	38.0
16-17	27.85243165549262	32.5	16.0	37.5	11.0	38.0
18-19	28.377027167591798	34.0	21.0	38.0	11.0	38.0
20-21	27.361329916501973	31.0	16.0	37.0	11.0	38.0
22-23	27.46906885041276	31.0	16.0	37.5	11.0	38.0
24-25	28.03817457990941	33.0	21.0	37.5	11.0	38.0
26-27	26.97190097493168	30.5	16.0	37.0	11.0	38.0
28-29	28.35398246825089	34.0	21.0	38.0	11.0	38.0
30-31	28.776016302446315	34.0	21.0	38.0	11.0	38.0
32-33	28.92718744976407	34.5	21.0	38.0	11.0	38.0
34-35	28.983059261850954	35.0	21.0	38.0	11.0	38.0
36-37	27.6545895547087	32.5	16.0	37.5	11.0	38.0
38-39	26.71806413178126	27.0	16.0	37.0	11.0	38.0
40-41	26.992099365490635	31.0	15.0	37.0	11.0	38.0
42-43	27.768943083280536	32.5	16.0	37.5	11.0	38.0
44-45	29.011399419390834	34.5	21.0	38.0	11.0	38.0
46-47	28.066481640835548	33.5	16.0	38.0	11.0	38.0
48-49	27.40082836096113	32.5	16.0	37.5	11.0	38.0
50-51	25.994576883433727	27.0	11.0	37.0	11.0	38.0
52-53	28.06096396251572	34.0	21.0	37.5	11.0	38.0
54-55	27.475825287704133	32.5	16.0	37.5	11.0	38.0
56-57	27.871533129710357	32.5	16.0	38.0	11.0	38.0
58-59	28.549668561053796	34.0	21.0	38.0	11.0	38.0
60-61	29.07143667672173	36.0	21.0	38.0	11.0	38.0
62-63	29.037418085880986	35.5	21.0	38.0	11.0	38.0
64-65	28.842757988104132	35.0	21.0	38.0	11.0	38.0
66-67	27.39064406010345	32.0	18.5	37.5	11.0	38.0
68-69	27.359599436411948	32.0	19.5	37.5	11.0	38.0
70-71	27.125549640192528	31.5	15.5	37.0	11.0	38.0
72-73	26.29306105852427	27.5	11.0	37.0	11.0	38.0
74-75	27.342152792881393	32.5	16.0	37.5	11.0	38.0
76	27.484042704087905	33.0	18.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	2.0
5	0.0
6	1.0
7	0.0
8	0.0
9	0.0
10	0.0
11	13.0
12	88.0
13	466.0
14	1170.0
15	2045.0
16	3402.0
17	4949.0
18	6585.0
19	6661.0
20	5851.0
21	4473.0
22	3267.0
23	2437.0
24	2013.0
25	1930.0
26	1818.0
27	2054.0
28	2207.0
29	2445.0
30	2942.0
31	3528.0
32	4342.0
33	5829.0
34	7778.0
35	10034.0
36	11182.0
37	6237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.499929078684836	17.399362653781054	14.817826781779841	35.28288148575427
2	17.117568628192643	14.316649487948105	45.47947537139129	23.086306512467967
3	17.430568032453593	14.240054467570046	42.631275354370175	25.69810214560619
4	14.268557919621749	22.250591016548462	28.364066193853425	35.11678486997636
5	13.963934147194825	25.93261529441802	41.18542614254238	18.918024415844766
6	26.016775255080333	32.746735255458574	15.624438539588278	25.612050949872817
7	24.217265084963735	21.844710688315004	19.957258087393974	33.98076613932729
8	20.14827984037221	34.10720027235073	17.097573430673503	28.646946456603562
9	20.765600915392397	14.740843711878352	18.26245661815465	46.231098754574596
10-11	23.143047628954697	22.429062504432853	27.29929216176728	27.12859770484517
12-13	21.51743116663278	16.796618263834056	31.15575750984685	30.53019305968632
14-15	24.550453683608286	21.488384848527833	22.363126563305293	31.598034904558585
16-17	28.051369587728797	18.584593852104383	23.40285691319088	29.96117964697594
18-19	20.761934483280374	18.48000832198517	28.58554622484491	32.172510969889544
20-21	27.576668841669267	17.922967081808466	24.72788826160553	29.77247581491674
22-23	29.23670510801878	17.078740939330753	23.53669896118511	30.147854991465355
24-25	22.037240179297562	22.83537911599493	21.21167703742931	33.915703667278194
26-27	26.47346695604068	21.975403208647176	23.92915064943662	27.621979185875524
28-29	23.33279745805988	22.408885442475366	23.732339757532202	30.525977341932553
30-31	22.47114507137487	21.83565102676735	25.33417813692438	30.3590257649334
32-33	23.0983824370777	21.00609482200188	25.043618877399037	30.85190386352138
34-35	23.142938200387725	21.0728639651993	25.10567875549671	30.67851907891626
36-37	26.997966806941225	17.02775544943023	29.951770769303515	26.022506974325026
38-39	26.411650669062364	15.32081895125065	34.757198921934844	23.51033145775214
40-41	24.08043993247812	22.494054008047776	24.045922444405566	29.37958361506854
42-43	19.355159011603167	23.066848232101147	27.27973370402943	30.298259052266257
44-45	19.463515660463003	18.732032077470116	30.514733696474504	31.289718565592374
46-47	25.81127150821083	17.826931897167228	27.299764999598086	29.062031595023857
48-49	25.13263038441534	18.192822355666934	29.744195943070594	26.930351316847133
50-51	32.40516144895055	16.491009073672167	27.692904189776396	23.410925287600893
52-53	18.526644285781835	20.897441959430708	29.608492127287345	30.96742162750012
54-55	24.545252516655555	18.905296206457958	26.48055945643077	30.06889182045572
56-57	24.4373309628733	19.266449794791292	25.481814914984962	30.81440432735044
58-59	19.096411177833467	21.733888127098208	28.549340394344885	30.620360300723437
60-61	19.142930095324555	20.095229989408384	27.884797246179456	32.87704266908761
62-63	19.157296464068544	22.0842041553578	27.37438884843441	31.384110532139243
64-65	19.288764060882023	23.079469102704135	26.751492971332112	30.88027386508173
66-67	17.56389342033714	29.07676667375937	20.816606378702982	32.54273352720051
68-69	18.161524054560246	28.52588963090486	21.023382962220246	32.28920335231465
70-71	17.77603351506239	30.070879061124323	20.38612302411023	31.766964399703056
72-73	16.613551468154522	31.726795593172252	19.822213816256088	31.837439122417138
74-75	18.662348101565087	26.157737954513216	24.901886614024306	30.278027329897395
76	19.42390255896204	24.669491044578518	26.88612335218353	29.020483044275906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	1.0
7	0.5
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.5
14	0.5
15	2.0
16	8.0
17	12.0
18	17.0
19	21.0
20	23.5
21	27.0
22	39.0
23	74.5
24	131.0
25	164.0
26	188.0
27	280.0
28	425.5
29	503.0
30	599.5
31	810.5
32	1065.5
33	1206.0
34	1352.0
35	1708.0
36	2124.5
37	2331.0
38	2539.0
39	2971.0
40	3466.0
41	3994.5
42	4252.0
43	4385.5
44	4714.5
45	4870.5
46	4831.0
47	4792.0
48	4690.5
49	4498.0
50	4368.0
51	4243.0
52	3972.5
53	3714.0
54	3601.0
55	3484.5
56	3354.0
57	3269.5
58	3199.0
59	3122.5
60	2952.0
61	2799.5
62	2741.0
63	2696.5
64	2574.0
65	2453.5
66	2340.0
67	2269.0
68	2233.5
69	2078.0
70	1911.0
71	1864.0
72	1783.5
73	1555.5
74	1293.0
75	1178.0
76	1080.0
77	936.5
78	739.5
79	588.0
80	484.0
81	333.0
82	241.5
83	197.0
84	159.5
85	85.5
86	33.5
87	18.0
88	12.5
89	7.0
90	5.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	9.456175355315789E-4
5	0.0
6	0.0
7	0.0
8	0.004728087677657895
9	0.0037824701421263155
10-11	0.0061465139809552625
12-13	0.0061465139809552625
14-15	0.005200896445423684
16-17	0.007092131516486841
18-19	0.006619322748721053
20-21	0.0037824701421263155
22-23	0.005200896445423684
24-25	0.004728087677657895
26-27	0.005200896445423684
28-29	0.004728087677657895
30-31	0.0061465139809552625
32-33	0.005200896445423684
34-35	0.005673705213189474
36-37	0.005673705213189474
38-39	0.005673705213189474
40-41	0.007092131516486841
42-43	0.0037824701421263155
44-45	0.006619322748721053
46-47	0.0061465139809552625
48-49	0.005673705213189474
50-51	0.005200896445423684
52-53	0.005673705213189474
54-55	0.005200896445423684
56-57	0.004728087677657895
58-59	0.005673705213189474
60-61	0.006619322748721053
62-63	0.007564940284252631
64-65	0.005200896445423684
66-67	0.008037749052018421
68-69	0.031205378672542104
70-71	0.007092131516486841
72-73	0.005673705213189474
74-75	0.005673705213189474
76	0.004728087677657895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	105751.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.61162060326632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.99218229070793	95.65175719846695
2	1.6798147246854804	3.2793887517955875
3	0.24700441182685307	0.7233150280372719
4	0.06199892472844572	0.2420726207361404
5	0.011999777889029251	0.05856588833136935
6	0.004999907260663616	0.029282943034764808
7	0.0	0.0
8	0.001999962901623566	0.015617569597910972
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0037824701421263155	0.0
2	0.0	0.0	0.0	0.0037824701421263155	0.0
3	0.0	0.0	0.0	0.0037824701421263155	0.0
4	0.0	0.0	0.0	0.011347410426378947	0.0
5	0.0	0.0	0.0	0.011347410426378947	0.0
6	0.0	0.0	0.0	0.011347410426378947	0.0
7	0.0	0.0	0.0	0.012293027961910525	0.0
8	0.0	0.0	0.0	0.013238645497442105	0.0
9	0.0	0.0	0.0	0.016075498104036842	0.0
10	0.0	0.0	0.0	0.0179667331751	0.0
11	0.0	0.0	0.0	0.0179667331751	0.0
12	0.0	0.0	0.0	0.0179667331751	0.0
13	0.0	0.0	0.0	0.0179667331751	0.0
14	0.0	0.0	0.0	0.01891235071063158	0.0
15	0.0	0.0	0.0	0.01891235071063158	0.0
16	0.0	0.0	0.0	0.022694820852757893	0.0
17	0.0	0.0	0.0	0.02553167345935263	0.0
18	0.0	0.0	0.0	0.027422908530415787	0.0
19	0.0	0.0	0.0	0.03498784881466842	0.0
20	0.0	0.0	0.0	0.04160717156338947	0.0
21	0.0	0.0	0.0	0.044444024169984206	0.0
22	0.0	0.0	0.0	0.06335637488061578	0.0
23	0.0	0.0	0.0	0.06524760995167894	0.0
24	0.0	0.0	0.0	0.07470378530699473	0.0
25	0.0	0.0	0.0	0.08415996066231052	0.0
26	0.0	0.0	0.0	0.09739860615975263	0.0
27	0.0	0.0	0.0	0.15319004075611578	0.0
28	0.0	0.0	0.0	0.19479721231950525	0.0
29	0.0	0.0	0.0	0.24775179430927366	0.0
30	0.0	0.0	0.0	0.3016519938345737	0.0
31	0.0	0.0	0.0	0.32907490236498943	0.0
32	0.0	0.0	0.0	0.383920719425821	0.0
33	0.0	0.0	0.0	0.45767888719728417	0.0
34	0.0	0.0	0.0	0.5777723142097947	0.0
35	0.0	0.0	0.0	0.8321434312677894	0.0
36	0.0	0.0	0.0	0.976822914204121	0.0
37	0.0	0.0	0.0	1.3550699284167527	0.0
38	0.0	0.0	0.0	1.7144045919187525	0.0
39	0.0	0.0	0.0	2.1087271042354208	0.0
40	0.0	0.0	0.0	2.5522217283997315	0.0
41	0.0	0.0	0.0	2.988151412279789	0.0
42	0.0	0.0	0.0	3.4883830885759948	0.0
43	0.0	0.0	0.0	3.931877712740305	0.0
44	0.0	0.0	0.0	4.468988472922242	0.0
45	0.0	0.0	0.0	4.767803614150221	0.0
46	0.0	0.0	0.0	5.181984094713052	0.0
47	0.0	0.0	0.0	5.63588051176821	0.0
48	0.0	0.0	0.0	5.912000832143431	0.0
49	0.0	0.0	0.0	6.153133303703984	0.0
50	0.0	0.0	0.0	6.522869760096831	0.0
51	0.0	0.0	0.0	6.915301037342436	0.0
52	0.0	0.0	0.0	7.231137294209984	0.0
53	0.0	0.0	0.0	7.66044765534132	0.0
54	0.0	0.0	0.0	8.093540486614783	0.0
55	0.0	0.0	0.0	8.40275742073361	0.0
56	0.0	0.0	0.0	8.728995470492006	0.0
57	0.0	0.0	0.0	8.98998591029872	0.0
58	0.0	0.0	0.0	9.243411409821183	0.0
59	0.0	0.0	0.0	9.548845873797884	0.0
60	0.0	0.0	0.0	9.72662197047782	0.0
61	0.0	0.0	0.0	9.920473565261794	0.0
62	0.0	0.0	0.0	10.038675757203242	0.0
63	0.0	0.0	0.0	10.20605006099233	0.0
64	0.0	0.0	0.0	10.387608627814394	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACCAT	15	0.0022193068	70.00001	56
GTCGTAT	35	3.6707206E-9	70.00001	54
CGTGTAC	40	1.05483196E-8	61.25	35
TACGCTT	35	2.9500006E-7	60.000004	14
CCGTGTA	80	0.0	56.875	34
GTGTAGC	80	0.0	56.875	36
TCGTGTA	75	0.0	56.0	34
CTTAAAG	25	2.3810889E-4	55.999996	42
TTCGTGT	50	9.677024E-10	55.999996	33
ATGTATA	25	2.3810889E-4	55.999996	28
TTTAGAT	80	0.0	52.5	37
GAGTACT	20	0.0069346814	52.5	36
TAGCACT	20	0.0069346814	52.5	39
TAGATCG	20	0.0069346814	52.5	41
GCGTCTA	20	0.0069346814	52.5	36
CCGTTCA	20	0.0069346814	52.5	36
GCGTACT	20	0.0069346814	52.5	36
ACGACTT	20	0.0069346814	52.5	24
TGTGTAG	100	0.0	52.499996	35
AGTGTAG	100	0.0	52.499996	35
>>END_MODULE