FastQCFastQC Report
Wed 25 May 2016
SRR1779744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105751
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2847526.92645932426171No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG34053.219827708485026No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC20251.9148755094514474No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT11911.1262304848181104TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC8060.7621677336384526No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC7610.7196149445395315TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC4810.45484203459068945RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT3690.34893287061115263No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2700.2553167345935263No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2630.24869741184480523No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2600.2458605592382105TruSeq Adapter, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2160.20425338767482104No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1970.18628665449972104RNA PCR Primer, Index 12 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG1450.13711454265207895No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT1220.11536533933485262No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1210.11441972179932106No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT1210.11441972179932106No Hit
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT1140.1078003990506No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAAGC150.002219306870.0000125
AAAGCCG150.002219306870.0000169
TTTACAC600.064.166672
TCTTTAT1150.057.826091
CTCGAAA252.3810889E-455.99999657
TGAACGA200.006934681452.55
TATGATA200.006934681452.554
GTTAAAA200.006934681452.519
ATATAAC200.006934681452.553
CTTTACA750.051.3333321
GCTTGAA44050.051.1691356
CTTTATA1300.051.153852
TATGCCG44450.051.10236443
TCTCGTA44000.051.06818438
CGTATGC44500.051.04494541
GTATGCC44500.051.04494542
TCGTATG44500.051.04494540
CTCGTAT44450.051.02362439
CCGTCTT44400.051.00225447
ATGCCGT44550.050.9876544
Produced by FastQC (version 0.11.5)