##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779744_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105751
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.65262739832247	34.0	34.0	34.0	34.0	34.0
2	33.65794176887216	34.0	34.0	34.0	34.0	34.0
3	33.67060358767293	34.0	34.0	34.0	34.0	34.0
4	33.71371429111782	34.0	34.0	34.0	34.0	34.0
5	33.71086798233586	34.0	34.0	34.0	34.0	34.0
6	37.393707860918575	38.0	38.0	38.0	37.0	38.0
7	37.37118325122221	38.0	38.0	38.0	37.0	38.0
8	37.259193766489204	38.0	38.0	38.0	36.0	38.0
9	37.29172300971149	38.0	38.0	38.0	36.0	38.0
10-11	37.182958080774654	38.0	38.0	38.0	36.0	38.0
12-13	37.15527512742196	38.0	38.0	38.0	36.0	38.0
14-15	37.19444260574368	38.0	38.0	38.0	36.0	38.0
16-17	37.09575795973561	38.0	38.0	38.0	35.5	38.0
18-19	37.077167118987056	38.0	38.0	38.0	35.5	38.0
20-21	37.13674102372555	38.0	38.0	38.0	36.0	38.0
22-23	37.11266560127091	38.0	38.0	38.0	36.0	38.0
24-25	37.05096878516515	38.0	38.0	38.0	35.5	38.0
26-27	36.98252971603105	38.0	38.0	38.0	35.0	38.0
28-29	36.88814290172197	38.0	37.5	38.0	34.5	38.0
30-31	36.6328781760929	38.0	37.0	38.0	34.0	38.0
32-33	36.63256612230617	38.0	37.0	38.0	34.0	38.0
34-35	36.381395920605954	38.0	37.0	38.0	34.0	38.0
36-37	36.411934638915945	38.0	37.0	38.0	34.0	38.0
38-39	35.57745553233539	38.0	36.0	38.0	31.5	38.0
40-41	36.50690300800938	38.0	37.0	38.0	34.0	38.0
42-43	36.8690461555919	38.0	37.0	38.0	35.0	38.0
44-45	36.86808162570567	38.0	37.5	38.0	34.5	38.0
46-47	36.89714518066023	38.0	38.0	38.0	35.0	38.0
48-49	36.83947669525584	38.0	37.5	38.0	34.5	38.0
50-51	36.71431948634056	38.0	37.0	38.0	34.0	38.0
52-53	36.719789883783605	38.0	37.0	38.0	34.0	38.0
54-55	36.732409149795274	38.0	37.0	38.0	34.5	38.0
56-57	36.7184565630585	38.0	37.0	38.0	34.0	38.0
58-59	36.59288328242759	38.0	37.0	38.0	34.0	38.0
60-61	36.61005096878516	38.0	37.0	38.0	34.0	38.0
62-63	36.684830403495	38.0	37.0	38.0	34.0	38.0
64-65	36.73332167071706	38.0	37.0	38.0	34.0	38.0
66-67	36.59725203544174	38.0	37.0	38.0	34.0	38.0
68-69	36.24858866582822	38.0	37.0	38.0	34.0	38.0
70-71	33.73806394265775	37.5	32.5	38.0	21.0	38.0
72-73	29.66361547408535	37.0	22.0	38.0	11.0	38.0
74-75	29.149435939140055	37.0	21.0	38.0	11.0	38.0
76	28.89773146353226	36.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	5.0
22	10.0
23	29.0
24	31.0
25	73.0
26	157.0
27	249.0
28	443.0
29	790.0
30	1311.0
31	2068.0
32	3013.0
33	5020.0
34	8772.0
35	20025.0
36	21201.0
37	42554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.742063904833053	11.749297878979869	11.788068197936663	49.72057001825042
2	14.299628372308536	11.177199269983262	52.5706612703426	21.952511087365604
3	14.464165823491031	13.535569403599021	50.341840739094664	21.658424033815283
4	11.053323372828626	38.41192991082827	26.215354937541964	24.319391778801148
5	12.022581346748494	25.47304517214967	49.740427986496584	12.763945494605252
6	22.264564874091025	50.426000699756976	13.076944898866206	14.232489527285793
7	20.011158286919272	22.01113937456856	14.345963631549582	43.63173870696258
8	17.252791935773658	50.96216584240339	11.842914014997493	19.94212820682547
9	16.292990137209102	14.288280961882158	11.161123771879227	58.257605129029514
10-11	19.07594254427854	31.118854668040964	28.725969494378305	21.07923329330219
12-13	18.532212461347882	12.995148982042723	31.863528477272084	36.609110079337306
14-15	18.23528855519097	12.395627464515702	15.351155071819653	54.01792890847368
16-17	34.994940946184904	28.449376365235317	12.494444496978751	24.061238191601024
18-19	35.826138759917164	17.003621715161085	12.742196291288025	34.42804323363372
20-21	19.615417348299307	13.84667757278891	12.49349887944322	54.04440619946856
22-23	18.930317443806675	30.231865419712346	12.53841571238097	38.29940142410001
24-25	35.25025768077843	30.328791217104328	12.276479655038724	22.144471447078516
26-27	35.56372989380715	32.74484402038752	13.4391164149748	18.252309670830535
28-29	19.06837760399429	17.708579587899877	28.965210730867792	34.25783207723804
30-31	17.984699908275097	29.24322228631408	18.253728097133834	34.51834970827699
32-33	17.7137804843453	16.034836550008983	44.96647785836541	21.28490510728031
34-35	33.575569025352	29.643691312611704	19.308091649251544	17.47264801278475
36-37	18.936936766555398	48.323892918270275	14.742650187705081	17.996520127469243
38-39	16.775255080330208	18.3066826791236	31.37748106400885	33.54058117653734
40-41	17.336006278900438	12.867963423513725	33.23230985995404	36.563720437631794
42-43	33.302285557583374	12.916189917825834	32.288110750725764	21.493413773865022
44-45	18.180915546897904	29.149606150296453	34.18029143932445	18.489186863481198
46-47	32.64129890024681	14.337453073729801	19.561989957541773	33.459258068481624
48-49	31.929721704759295	13.098694102183432	16.477385556637763	38.49419863641951
50-51	18.28966156348403	13.28639918298645	33.31930667322295	35.10463258030657
52-53	14.749269510453802	13.397982052179175	49.576836152849616	22.275912284517403
54-55	15.093001484619531	13.214532250286048	36.18831027602576	35.50415598906866
56-57	31.305614131308452	13.34360904388611	20.314228707057143	35.036548117748296
58-59	17.089200102126696	14.515229170409736	46.30216262730376	22.09340810015981
60-61	31.179374190314984	30.765666518519918	19.299581091431758	18.755378199733336
62-63	15.760607464704826	51.47705459050032	13.887339126816766	18.87499881797808
64-65	14.405537536288074	53.2160452383429	13.535096594831256	18.84332063053777
66-67	14.46652986732986	53.59523787009106	13.00555077493357	18.932681487645507
68-69	14.486387835576023	53.65102930468743	13.168196991045003	18.694385868691548
70-71	14.800805666140274	50.335221416345945	14.285916918043329	20.578055999470454
72-73	15.54784351921022	41.39157076528827	17.578084367996517	25.48250134750499
74-75	16.27218655142741	37.80011536533934	19.456080793562236	26.471617289671016
76	15.994174995981126	38.66062732267307	19.204546529110836	26.140651152234966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	5.0
16	6.5
17	7.0
18	9.0
19	10.0
20	31.5
21	54.0
22	50.0
23	60.0
24	110.0
25	146.0
26	164.0
27	204.5
28	284.5
29	342.0
30	360.0
31	450.5
32	639.0
33	755.0
34	770.0
35	910.0
36	1321.5
37	1608.0
38	7295.0
39	13724.0
40	12760.5
41	9108.5
42	7162.0
43	5665.5
44	3556.0
45	2754.0
46	2565.0
47	2415.0
48	2131.0
49	1950.0
50	1903.0
51	1781.0
52	1576.5
53	1481.5
54	1469.0
55	1535.0
56	1543.5
57	1624.5
58	1763.0
59	1750.0
60	1811.5
61	1934.5
62	1983.0
63	1888.5
64	1828.0
65	1872.5
66	1893.0
67	1903.0
68	1908.0
69	1853.5
70	1814.0
71	1834.0
72	1773.0
73	1653.5
74	1515.0
75	1435.0
76	1357.5
77	1197.0
78	1013.5
79	913.0
80	825.5
81	624.0
82	400.0
83	290.0
84	240.5
85	149.5
86	82.0
87	56.0
88	41.0
89	22.5
90	10.5
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	105751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.034221898610888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.318992047289676	15.305765430114135
2	25.63758798257107	15.912851887925411
3	13.525701575306986	12.592788720674037
4	6.066607757701332	7.530898052973495
5	2.6356683628385995	4.0897958411740785
6	1.2188061793473293	2.269482085275789
7	0.5819799506383497	1.264290645005721
8	0.34431274566562053	0.8548382521205473
9	0.1553977878667845	0.4340384488089947
>10	0.43877022456503856	2.125748219874989
>50	0.021329108138578263	0.41701733316942635
>100	0.036564185380419875	2.5333093776890996
>500	0.006094030896736646	1.4817826781779841
>1k	0.009141046345104969	6.260933702754584
>5k	0.0	0.0
>10k+	0.003047015448368323	26.92645932426171
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	28475	26.92645932426171	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	3405	3.219827708485026	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	2025	1.9148755094514474	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	1191	1.1262304848181104	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	806	0.7621677336384526	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	761	0.7196149445395315	TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	481	0.45484203459068945	RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	369	0.34893287061115263	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	270	0.2553167345935263	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	263	0.24869741184480523	No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	260	0.2458605592382105	TruSeq Adapter, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	216	0.20425338767482104	No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT	197	0.18628665449972104	RNA PCR Primer, Index 12 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG	145	0.13711454265207895	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	122	0.11536533933485262	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	121	0.11441972179932106	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT	121	0.11441972179932106	No Hit
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	114	0.1078003990506	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006619322748721053	0.0
2	0.0	0.0	0.0	0.006619322748721053	0.0
3	0.0	0.0	0.0	0.006619322748721053	0.0
4	0.0	0.0	0.0	0.015129880568505262	0.0
5	0.0	0.0	0.0	0.015129880568505262	0.0
6	0.0	0.0	0.0	0.015129880568505262	0.0
7	0.0	0.0	0.0	0.016075498104036842	0.0
8	0.0	0.0	0.0	0.01891235071063158	0.0
9	0.0	0.0	0.0	0.02458605592382105	0.0
10	0.0	0.0	0.0	0.02647729099488421	0.0
11	0.0	0.0	0.0	0.02647729099488421	0.0
12	0.0	0.0	0.0	0.033096613743605265	0.0
13	0.0	0.0	0.0	0.033096613743605265	0.0
14	0.0	0.0	0.0	0.03404223127913684	0.0
15	0.0	0.0	0.0	0.03404223127913684	0.0
16	0.0	0.0	0.0	0.03687908388573158	0.0
17	0.0	0.0	0.0	0.04066155402785789	0.0
18	0.0	0.0	0.0	0.045389641705515786	0.0
19	0.0	0.0	0.0	0.05295458198976842	0.0
20	0.0	0.0	0.0	0.06146513980955263	0.0
21	0.0	0.0	0.0	0.06903008009380526	0.0
22	0.0	0.0	0.0	0.07375816777146316	0.0
23	0.0	0.0	0.0	0.08037749052018421	0.0
24	0.0	0.0	0.0	0.08699681326890527	0.0
25	0.0	0.0	0.0	0.09645298862422105	0.0
26	0.0	0.0	0.0	0.1125284867282579	0.0
27	0.0	0.0	0.0	0.179667331751	0.0
28	0.0	0.0	0.0	0.22883944359864208	0.0
29	0.0	0.0	0.0	0.32150996208073684	0.0
30	0.0	0.0	0.0	0.3905400421745421	0.0
31	0.0	0.0	0.0	0.46902629762366316	0.0
32	0.0	0.0	0.0	0.5607511985702263	0.0
33	0.0	0.0	0.0	0.7035394464354947	0.0
34	0.0	0.0	0.0	0.9853334720239052	0.0
35	0.0	0.0	0.0	1.360743633629942	0.0
36	0.0	0.0	0.0	1.793836464903405	0.0
37	0.0	0.0	0.0	2.376336866790858	0.0
38	0.0	0.0	0.0	2.9966619700995736	0.0
39	0.0	0.0	0.0	3.5952378700910628	0.0
40	0.0	0.0	0.0	4.265680702782952	0.0
41	0.0	0.0	0.0	4.866147837845505	0.0
42	0.0	0.0	0.0	5.566850431674405	0.0
43	0.0	0.0	0.0	6.1833930648409945	0.0
44	0.0	0.0	0.0	6.862346455352668	0.0
45	0.0	0.0	0.0	7.442010004633526	0.0
46	0.0	0.0	0.0	8.136093275713705	0.0
47	0.0	0.0	0.0	8.74507096859604	0.0
48	0.0	0.0	0.0	9.293529139204358	0.0
49	0.0	0.0	0.0	9.809836313604599	0.0
50	0.0	0.0	0.0	10.272243288479542	0.0
51	0.0	0.0	0.0	10.726139705534699	0.0
52	0.0	0.0	0.0	11.18760106287411	0.0
53	0.0	0.0	0.0	11.655681742962242	0.0
54	0.0	0.0	0.0	12.142674773761005	0.0
55	0.0	0.0	0.0	12.60035366095829	0.0
56	0.0	0.0	0.0	13.054250078013446	0.0
57	0.0	0.0	0.0	13.475049881324999	0.0
58	0.0	0.0	0.0	13.9062514775274	0.0
59	0.0	0.0	0.0	14.3374530737298	0.0
60	0.0	0.0	0.0	14.734612438653063	0.0
61	0.0	0.0	0.0	15.126098098363135	0.0
62	0.0	0.0	0.0	15.489215232007263	0.0
63	0.0	0.0	0.0	15.844767425367136	0.0
64	0.0	0.0	0.0	16.243818025361463	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAAGC	15	0.0022193068	70.00001	25
AAAGCCG	15	0.0022193068	70.00001	69
TTTACAC	60	0.0	64.16667	2
TCTTTAT	115	0.0	57.82609	1
CTCGAAA	25	2.3810889E-4	55.999996	57
TGAACGA	20	0.0069346814	52.5	5
TATGATA	20	0.0069346814	52.5	54
GTTAAAA	20	0.0069346814	52.5	19
ATATAAC	20	0.0069346814	52.5	53
CTTTACA	75	0.0	51.333332	1
GCTTGAA	4405	0.0	51.16913	56
CTTTATA	130	0.0	51.15385	2
TATGCCG	4445	0.0	51.102364	43
TCTCGTA	4400	0.0	51.068184	38
CGTATGC	4450	0.0	51.044945	41
GTATGCC	4450	0.0	51.044945	42
TCGTATG	4450	0.0	51.044945	40
CTCGTAT	4445	0.0	51.023624	39
CCGTCTT	4440	0.0	51.002254	47
ATGCCGT	4455	0.0	50.98765	44
>>END_MODULE